In vitro biocompatibility of calcined mesoporous silica particles and fetal blood cells

Mohammed T. Al Samri, Ankush V. Biradar, Ahmed R. Alsuwaidi, Ghazala Balhaj, Suleiman Al-Hammadi, Safa Shehab, Suhail Al-Salam, Saeed Tariq, Thachillath Pramathan, Sheela Benedict, Teddy Asefa, Abdul Kader Souid

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background: The biocompatibility of two forms of calcined mesoporous silica particles, labeled as MCM41-cal and SBA15-cal, with fetal blood mononuclear cells was assessed in vitro. Methods and results: Fetal mononuclear cells were isolated from umbilical cord blood and exposed to 0.5 mg/mL of MCM41-cal or SBA15-cal for several hours. Transmission electron micrographs confirmed the presence of particles in the cytosol of macrophages, neutrophils, and lymphocytes without noticeable damage to the cellular organelles. The particles (especially MCM41-cal) were in close proximity to plasma, and nuclear and mitochondrial membranes. Biocompatibility was assessed by a functional assay that measured cellular respiration, ie, mitochondrial O2 consumption. The rate of respiration (kc, in μM O2 per minute per 107 cells) for untreated cells was 0.42 ± 0.16 (n= 10), for cells treated with MCM41-cal was 0.39 ± 0.22 (n= 5, P> 0.966) and for cells treated with SBA15-cal was 0.44 ± 0.13 (n= 5, P> 0.981). Conclusion: The results show reasonable biocompatibility of MCM41-cal and SBA15-cal in fetal blood mononuclear cells. Future studies are needed to determine the potential of collecting fetal cells from a fetus or neonate, loading the cells in vitro with therapeutic MCM41-cal or SBA15-cal, and reinfusing them into the fetus or neonate.

Original languageEnglish
Pages (from-to)3111-3121
Number of pages11
JournalInternational Journal of Nanomedicine
Volume7
DOIs
Publication statusPublished - 2012

Fingerprint

Fetal Blood
Biocompatibility
Silicon Dioxide
Blood Cells
Blood
Silica
Cells
Lymphocytes
Macrophages
Assays
Fetus
Membranes
Plasmas
Cell Respiration
Electrons
Nuclear Envelope
Mitochondrial Membranes
Respiratory Rate
In Vitro Techniques
Organelles

Keywords

  • Biocompatibility
  • Bioenergetics
  • Fetal cells
  • In vitro
  • Mesoporous silica
  • Nanomaterials

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Organic Chemistry
  • Drug Discovery

Cite this

Al Samri, M. T., Biradar, A. V., Alsuwaidi, A. R., Balhaj, G., Al-Hammadi, S., Shehab, S., ... Souid, A. K. (2012). In vitro biocompatibility of calcined mesoporous silica particles and fetal blood cells. International Journal of Nanomedicine, 7, 3111-3121. https://doi.org/10.2147/IJN.S32711

In vitro biocompatibility of calcined mesoporous silica particles and fetal blood cells. / Al Samri, Mohammed T.; Biradar, Ankush V.; Alsuwaidi, Ahmed R.; Balhaj, Ghazala; Al-Hammadi, Suleiman; Shehab, Safa; Al-Salam, Suhail; Tariq, Saeed; Pramathan, Thachillath; Benedict, Sheela; Asefa, Teddy; Souid, Abdul Kader.

In: International Journal of Nanomedicine, Vol. 7, 2012, p. 3111-3121.

Research output: Contribution to journalArticle

Al Samri, MT, Biradar, AV, Alsuwaidi, AR, Balhaj, G, Al-Hammadi, S, Shehab, S, Al-Salam, S, Tariq, S, Pramathan, T, Benedict, S, Asefa, T & Souid, AK 2012, 'In vitro biocompatibility of calcined mesoporous silica particles and fetal blood cells', International Journal of Nanomedicine, vol. 7, pp. 3111-3121. https://doi.org/10.2147/IJN.S32711
Al Samri, Mohammed T. ; Biradar, Ankush V. ; Alsuwaidi, Ahmed R. ; Balhaj, Ghazala ; Al-Hammadi, Suleiman ; Shehab, Safa ; Al-Salam, Suhail ; Tariq, Saeed ; Pramathan, Thachillath ; Benedict, Sheela ; Asefa, Teddy ; Souid, Abdul Kader. / In vitro biocompatibility of calcined mesoporous silica particles and fetal blood cells. In: International Journal of Nanomedicine. 2012 ; Vol. 7. pp. 3111-3121.
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abstract = "Background: The biocompatibility of two forms of calcined mesoporous silica particles, labeled as MCM41-cal and SBA15-cal, with fetal blood mononuclear cells was assessed in vitro. Methods and results: Fetal mononuclear cells were isolated from umbilical cord blood and exposed to 0.5 mg/mL of MCM41-cal or SBA15-cal for several hours. Transmission electron micrographs confirmed the presence of particles in the cytosol of macrophages, neutrophils, and lymphocytes without noticeable damage to the cellular organelles. The particles (especially MCM41-cal) were in close proximity to plasma, and nuclear and mitochondrial membranes. Biocompatibility was assessed by a functional assay that measured cellular respiration, ie, mitochondrial O2 consumption. The rate of respiration (kc, in μM O2 per minute per 107 cells) for untreated cells was 0.42 ± 0.16 (n= 10), for cells treated with MCM41-cal was 0.39 ± 0.22 (n= 5, P> 0.966) and for cells treated with SBA15-cal was 0.44 ± 0.13 (n= 5, P> 0.981). Conclusion: The results show reasonable biocompatibility of MCM41-cal and SBA15-cal in fetal blood mononuclear cells. Future studies are needed to determine the potential of collecting fetal cells from a fetus or neonate, loading the cells in vitro with therapeutic MCM41-cal or SBA15-cal, and reinfusing them into the fetus or neonate.",
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AU - Al-Hammadi, Suleiman

AU - Shehab, Safa

AU - Al-Salam, Suhail

AU - Tariq, Saeed

AU - Pramathan, Thachillath

AU - Benedict, Sheela

AU - Asefa, Teddy

AU - Souid, Abdul Kader

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N2 - Background: The biocompatibility of two forms of calcined mesoporous silica particles, labeled as MCM41-cal and SBA15-cal, with fetal blood mononuclear cells was assessed in vitro. Methods and results: Fetal mononuclear cells were isolated from umbilical cord blood and exposed to 0.5 mg/mL of MCM41-cal or SBA15-cal for several hours. Transmission electron micrographs confirmed the presence of particles in the cytosol of macrophages, neutrophils, and lymphocytes without noticeable damage to the cellular organelles. The particles (especially MCM41-cal) were in close proximity to plasma, and nuclear and mitochondrial membranes. Biocompatibility was assessed by a functional assay that measured cellular respiration, ie, mitochondrial O2 consumption. The rate of respiration (kc, in μM O2 per minute per 107 cells) for untreated cells was 0.42 ± 0.16 (n= 10), for cells treated with MCM41-cal was 0.39 ± 0.22 (n= 5, P> 0.966) and for cells treated with SBA15-cal was 0.44 ± 0.13 (n= 5, P> 0.981). Conclusion: The results show reasonable biocompatibility of MCM41-cal and SBA15-cal in fetal blood mononuclear cells. Future studies are needed to determine the potential of collecting fetal cells from a fetus or neonate, loading the cells in vitro with therapeutic MCM41-cal or SBA15-cal, and reinfusing them into the fetus or neonate.

AB - Background: The biocompatibility of two forms of calcined mesoporous silica particles, labeled as MCM41-cal and SBA15-cal, with fetal blood mononuclear cells was assessed in vitro. Methods and results: Fetal mononuclear cells were isolated from umbilical cord blood and exposed to 0.5 mg/mL of MCM41-cal or SBA15-cal for several hours. Transmission electron micrographs confirmed the presence of particles in the cytosol of macrophages, neutrophils, and lymphocytes without noticeable damage to the cellular organelles. The particles (especially MCM41-cal) were in close proximity to plasma, and nuclear and mitochondrial membranes. Biocompatibility was assessed by a functional assay that measured cellular respiration, ie, mitochondrial O2 consumption. The rate of respiration (kc, in μM O2 per minute per 107 cells) for untreated cells was 0.42 ± 0.16 (n= 10), for cells treated with MCM41-cal was 0.39 ± 0.22 (n= 5, P> 0.966) and for cells treated with SBA15-cal was 0.44 ± 0.13 (n= 5, P> 0.981). Conclusion: The results show reasonable biocompatibility of MCM41-cal and SBA15-cal in fetal blood mononuclear cells. Future studies are needed to determine the potential of collecting fetal cells from a fetus or neonate, loading the cells in vitro with therapeutic MCM41-cal or SBA15-cal, and reinfusing them into the fetus or neonate.

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