In vivo bicarbonate requirement for water oxidation by Photosystem II in the hypercarbonate-requiring cyanobacterium Arthrospira maxima

Damian Carrieri, Gennady Ananyev, Tyler Brown, G Charles Dismukes

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

While the presence of inorganic carbon in the form of (bi)carbonate has been known to be important for activity of Photosystem II (PSII), the vast majority of studies on this "bicarbonate effect" have been limited to in vitro studies of isolated thylakoid membranes and PSII complexes. Here we report an in vivo requirement for bicarbonate that is both reversible and selective for this anion for efficient water oxidation activity in the hypercarbonate-requiring cyanobacterium Arthrospira (Spirulina) maxima, originally isolated from highly alkaline soda lakes. Using a non-invasive internal probe of PSII charge separation (variable fluorescence), primary electron acceptor (QA - / QA) reoxidation rate, and flash-induced oxygen yield, we report the largest reversible bicarbonate effect on PSII activity ever observed, which is due to the requirement for bicarbonate at the water-oxidizing complex. Temporal separation of this donor side bicarbonate requirement from a smaller effect of bicarbonate on the QA - reoxidation rate was observed. We expect the atypical way in which Arthrospira manages intracellular pH, sodium, and inorganic carbon concentrations relative to other cyanobacteria is responsible for this strong in vivo bicarbonate requirement.

Original languageEnglish
Pages (from-to)1865-1874
Number of pages10
JournalJournal of Inorganic Biochemistry
Volume101
Issue number11-12
DOIs
Publication statusPublished - Nov 2007

Fingerprint

Spirulina
Photosystem II Protein Complex
Cyanobacteria
Bicarbonates
Oxidation
Water
Carbon
Thylakoids
Carbonates
Lakes
Anions
Fluorescence
Sodium
Electrons
Oxygen
Membranes

Keywords

  • Arthrospira (Spirulina)
  • Bicarbonate
  • Cyanobacteria
  • Oxygenic photosynthesis
  • Photosystem II

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry

Cite this

In vivo bicarbonate requirement for water oxidation by Photosystem II in the hypercarbonate-requiring cyanobacterium Arthrospira maxima. / Carrieri, Damian; Ananyev, Gennady; Brown, Tyler; Dismukes, G Charles.

In: Journal of Inorganic Biochemistry, Vol. 101, No. 11-12, 11.2007, p. 1865-1874.

Research output: Contribution to journalArticle

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