In vivo interactions between photosynthesis, mitorespiration, and chlororespiration in Chlamydomonas reinhardtii

Laurent Cournac, Gwendal Latouche, Zoran Cerovic, Kevin Edward Redding, Jacques Ravenel, Gilles Peltier

Research output: Contribution to journalArticle

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Abstract

Interactions between photosynthesis, mitochondrial respiration (mitorespiration), and chlororespiration have been investigated in the green alga Chlamydomonas reinhardtii using flash illumination and a bare platinum electrode. Depending on the physiological status of algae, flash illumination was found to induce either a fast (t1/2 ≈ 300 ms) or slow (t1/2 ≈ 3 s) transient inhibition of oxygen uptake. Based on the effects of the mitorespiratory inhibitors myxothiazol and salicyl hydroxamic acid (SHAM), and of propyl gallate, an inhibitor of the chlororespiratory oxidase, we conclude that the fast transient is due to the flash-induced inhibition of chlororespiration and that the slow transient is due to the flash-induced inhibition of mitorespiration. By measuring blue-green fluorescence changes, related to the redox status of the pyridine nucleotide pool, and chlorophyll fluorescence, related to the redox status of plastoquinones (PQs) in C. reinhardtii wild type and in a photosystem I-deficient mutant, we show that interactions between photosynthesis and chlororespiration are favored when PQ and pyridine nucleotide pools are reduced, whereas interactions between photosynthesis and mitorespiration are favored at more oxidized states. We conclude that the plastid oxidase, similar to the mitochondrial alternative oxidase, becomes significantly engaged when the PQ pool becomes highly reduced, and thereby prevents its over-reduction.

Original languageEnglish
Pages (from-to)1921-1928
Number of pages8
JournalPlant Physiology
Volume129
Issue number4
DOIs
Publication statusPublished - Aug 2 2002

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Plastoquinone
Chlamydomonas reinhardtii
Photosynthesis
pyridine nucleotides
Respiration
photosynthesis
Lighting
algae
Oxidation-Reduction
lighting
Oxidoreductases
Nucleotides
Fluorescence
Propyl Gallate
fluorescence
hydroxamic acids
Hydroxamic Acids
propyl gallate
Photosystem I Protein Complex
platinum

ASJC Scopus subject areas

  • Plant Science

Cite this

In vivo interactions between photosynthesis, mitorespiration, and chlororespiration in Chlamydomonas reinhardtii. / Cournac, Laurent; Latouche, Gwendal; Cerovic, Zoran; Redding, Kevin Edward; Ravenel, Jacques; Peltier, Gilles.

In: Plant Physiology, Vol. 129, No. 4, 02.08.2002, p. 1921-1928.

Research output: Contribution to journalArticle

Cournac, Laurent ; Latouche, Gwendal ; Cerovic, Zoran ; Redding, Kevin Edward ; Ravenel, Jacques ; Peltier, Gilles. / In vivo interactions between photosynthesis, mitorespiration, and chlororespiration in Chlamydomonas reinhardtii. In: Plant Physiology. 2002 ; Vol. 129, No. 4. pp. 1921-1928.
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