In vivo migration of endogenous brain progenitor cells guided by an injectable peptide amphiphile biomaterial

Reza Motalleb, Eric J. Berns, Piyush Patel, Julie Gold, Samuel I Stupp, H. Georg Kuhn

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Biomaterials hold great promise in helping the adult brain regenerate and rebuild after trauma. Peptide amphiphiles (PAs) are highly versatile biomaterials, gelling and forming macromolecular structures when exposed to physiological levels of electrolytes. We are here reporting on the first ever in vivo use of self-assembling PA carrying a Tenascin-C signal (E2Ten-C PA) for the redirection of endogenous neuroblasts in the rodent brain. The PA forms highly aligned nanofibers, displaying the migratory sequence of Tenascin-C glycoprotein as epitope. In this in vivo work, we have formed in situ a gel of aligned PA nanofibers presenting a migratory Tenascin-C signal sequence in the ventral horn of the rostral migratory stream, creating a track reaching the neocortex. Seven days posttransplant, doublecortin positive cells were observed migrating inside and alongside the injected biomaterial, reaching the cortex. We observed a 24-fold increase in number of redirected neuroblasts for the E2Ten-C PA–injected animals compared to control. We also found injecting the E2Ten-C PA to cause minimal neuroinflammatory response. Analysing GFAP+ astrocytes and Iba1+ microglia activation, the PA does not elicit a stronger neuroinflammatory response than would be expected from a small needle stab wound. Redirecting endogenous neuroblasts and increasing the number of cells reaching a site of injury using PAs may open up new avenues for utilizing the pool of neuroblasts and neural stem cells within the adult brain for regenerating damaged brain tissue and replacing neurons lost to injury.

Original languageEnglish
Pages (from-to)e2123-e2133
JournalJournal of Tissue Engineering and Regenerative Medicine
Volume12
Issue number4
DOIs
Publication statusPublished - Apr 1 2018

Fingerprint

Amphiphiles
Biocompatible Materials
Biomaterials
Peptides
Brain
Stem Cells
Tenascin
Injections
Nanofibers
C-Peptide
Wounds and Injuries
Stab Wounds
Neural Stem Cells
Neocortex
Microglia
Horns
Protein Sorting Signals
Epitopes
Astrocytes
Glycoproteins

Keywords

  • cell migration
  • doublecortin
  • nanofiber hydrogel
  • peptide amphiphile
  • rostral migratory stream
  • Tenascin-C

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

Cite this

In vivo migration of endogenous brain progenitor cells guided by an injectable peptide amphiphile biomaterial. / Motalleb, Reza; Berns, Eric J.; Patel, Piyush; Gold, Julie; Stupp, Samuel I; Kuhn, H. Georg.

In: Journal of Tissue Engineering and Regenerative Medicine, Vol. 12, No. 4, 01.04.2018, p. e2123-e2133.

Research output: Contribution to journalArticle

Motalleb, Reza ; Berns, Eric J. ; Patel, Piyush ; Gold, Julie ; Stupp, Samuel I ; Kuhn, H. Georg. / In vivo migration of endogenous brain progenitor cells guided by an injectable peptide amphiphile biomaterial. In: Journal of Tissue Engineering and Regenerative Medicine. 2018 ; Vol. 12, No. 4. pp. e2123-e2133.
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