Influence of tumour depth, blood absorption and autofluorescence on measurements of exogenous fluorophores in tissue

A. E. Saarnak; T. Rodrigues; J. Schwartz; A. L. Moore; T. A. Moore; D. Gust; M. J.C. Van Gemert; H. J.C.M. Sterenborg; S. Thomsen

doi:10.1007/BF00592957

Influence of tumour depth, blood absorption and autofluorescence on measurements of exogenous fluorophores in tissue

A. E. Saarnak, T. Rodrigues, J. Schwartz, A. L. Moore, T. A. Moore, D. Gust, M. J.C. Van Gemert, H. J.C.M. Sterenborg, S. Thomsen

Arizona State University

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

We investigated the influence of tumour depth and differences in blood concentration and autofluorescence between tumour and normal tissue on the fluorescence of a tumour-localising agent. Carotenoporphyrin, CP(Me)₃, was injected into rats and nude mice with intradermal tumours. On the tumours an incision was made, uncovering 2 mm² of the tumour, and fluorescence measurements, including excitation-emission maps and fluorescence ratios, were made on skin, covered and uncovered tumour. The measured fluorescence ratio in the uncovered tumour showed a three- to tenfold increase compared to the covered tumour. We used a one-dimensional layered tissue model to analyse the data. In conclusion, even with a high tumour-selectivity deeper lying tumours cannot always be detected, particularly if the tumour has a high blood concentration or low autofluorescence intensity.

Original language	English
Pages (from-to)	22-31
Number of pages	10
Journal	Lasers in Medical Science
Volume	13
Issue number	1
DOIs	https://doi.org/10.1007/BF00592957
Publication status	Published - 1998

Keywords

Carothenoporphyrin
Fluorescence ratio
Fluorescence spectroscopy
Tissue optics
Tumour detection

ASJC Scopus subject areas

Surgery
Dermatology

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10.1007/BF00592957

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Influence of tumour depth, blood absorption and autofluorescence on measurements of exogenous fluorophores in tissue. / Saarnak, A. E.; Rodrigues, T.; Schwartz, J.; Moore, A. L.; Moore, T. A.; Gust, D.; Van Gemert, M. J.C.; Sterenborg, H. J.C.M.; Thomsen, S.

In: Lasers in Medical Science, Vol. 13, No. 1, 1998, p. 22-31.

Research output: Contribution to journal › Article › peer-review

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title = "Influence of tumour depth, blood absorption and autofluorescence on measurements of exogenous fluorophores in tissue",

abstract = "We investigated the influence of tumour depth and differences in blood concentration and autofluorescence between tumour and normal tissue on the fluorescence of a tumour-localising agent. Carotenoporphyrin, CP(Me)3, was injected into rats and nude mice with intradermal tumours. On the tumours an incision was made, uncovering 2 mm2 of the tumour, and fluorescence measurements, including excitation-emission maps and fluorescence ratios, were made on skin, covered and uncovered tumour. The measured fluorescence ratio in the uncovered tumour showed a three- to tenfold increase compared to the covered tumour. We used a one-dimensional layered tissue model to analyse the data. In conclusion, even with a high tumour-selectivity deeper lying tumours cannot always be detected, particularly if the tumour has a high blood concentration or low autofluorescence intensity.",

keywords = "Carothenoporphyrin, Fluorescence ratio, Fluorescence spectroscopy, Tissue optics, Tumour detection",

author = "Saarnak, {A. E.} and T. Rodrigues and J. Schwartz and Moore, {A. L.} and Moore, {T. A.} and D. Gust and {Van Gemert}, {M. J.C.} and Sterenborg, {H. J.C.M.} and S. Thomsen",

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AU - Saarnak, A. E.

AU - Rodrigues, T.

AU - Schwartz, J.

AU - Moore, A. L.

AU - Moore, T. A.

AU - Gust, D.

AU - Van Gemert, M. J.C.

AU - Sterenborg, H. J.C.M.

AU - Thomsen, S.

N1 - Funding Information: This research was partly supported by the Netherlands Organization for Scientific Research (NWO), SIR-grant

PY - 1998

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N2 - We investigated the influence of tumour depth and differences in blood concentration and autofluorescence between tumour and normal tissue on the fluorescence of a tumour-localising agent. Carotenoporphyrin, CP(Me)3, was injected into rats and nude mice with intradermal tumours. On the tumours an incision was made, uncovering 2 mm2 of the tumour, and fluorescence measurements, including excitation-emission maps and fluorescence ratios, were made on skin, covered and uncovered tumour. The measured fluorescence ratio in the uncovered tumour showed a three- to tenfold increase compared to the covered tumour. We used a one-dimensional layered tissue model to analyse the data. In conclusion, even with a high tumour-selectivity deeper lying tumours cannot always be detected, particularly if the tumour has a high blood concentration or low autofluorescence intensity.

AB - We investigated the influence of tumour depth and differences in blood concentration and autofluorescence between tumour and normal tissue on the fluorescence of a tumour-localising agent. Carotenoporphyrin, CP(Me)3, was injected into rats and nude mice with intradermal tumours. On the tumours an incision was made, uncovering 2 mm2 of the tumour, and fluorescence measurements, including excitation-emission maps and fluorescence ratios, were made on skin, covered and uncovered tumour. The measured fluorescence ratio in the uncovered tumour showed a three- to tenfold increase compared to the covered tumour. We used a one-dimensional layered tissue model to analyse the data. In conclusion, even with a high tumour-selectivity deeper lying tumours cannot always be detected, particularly if the tumour has a high blood concentration or low autofluorescence intensity.

KW - Carothenoporphyrin

KW - Fluorescence ratio

KW - Fluorescence spectroscopy

KW - Tissue optics

KW - Tumour detection

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