Influence of tumour depth, blood absorption and autofluorescence on measurements of exogenous fluorophores in tissue

A. E. Saarnak, T. Rodrigues, J. Schwartz, A. L. Moore, T. A. Moore, D. Gust, M. J.C. Van Gemert, H. J.C.M. Sterenborg, S. Thomsen

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

We investigated the influence of tumour depth and differences in blood concentration and autofluorescence between tumour and normal tissue on the fluorescence of a tumour-localising agent. Carotenoporphyrin, CP(Me)3, was injected into rats and nude mice with intradermal tumours. On the tumours an incision was made, uncovering 2 mm2 of the tumour, and fluorescence measurements, including excitation-emission maps and fluorescence ratios, were made on skin, covered and uncovered tumour. The measured fluorescence ratio in the uncovered tumour showed a three- to tenfold increase compared to the covered tumour. We used a one-dimensional layered tissue model to analyse the data. In conclusion, even with a high tumour-selectivity deeper lying tumours cannot always be detected, particularly if the tumour has a high blood concentration or low autofluorescence intensity.

Original languageEnglish
Pages (from-to)22-31
Number of pages10
JournalLasers in Medical Science
Volume13
Issue number1
DOIs
Publication statusPublished - 1998

Keywords

  • Carothenoporphyrin
  • Fluorescence ratio
  • Fluorescence spectroscopy
  • Tissue optics
  • Tumour detection

ASJC Scopus subject areas

  • Surgery
  • Dermatology

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