Abstract
We investigated the influence of tumour depth and differences in blood concentration and autofluorescence between tumour and normal tissue on the fluorescence of a tumour-localising agent. Carotenoporphyrin, CP(Me)3, was injected into rats and nude mice with intradermal tumours. On the tumours an incision was made, uncovering 2 mm2 of the tumour, and fluorescence measurements, including excitation-emission maps and fluorescence ratios, were made on skin, covered and uncovered tumour. The measured fluorescence ratio in the uncovered tumour showed a three- to tenfold increase compared to the covered tumour. We used a one-dimensional layered tissue model to analyse the data. In conclusion, even with a high tumour-selectivity deeper lying tumours cannot always be detected, particularly if the tumour has a high blood concentration or low autofluorescence intensity.
| Original language | English |
|---|---|
| Pages (from-to) | 22-31 |
| Number of pages | 10 |
| Journal | Lasers in Medical Science |
| Volume | 13 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1998 |
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Keywords
- Carothenoporphyrin
- Fluorescence ratio
- Fluorescence spectroscopy
- Tissue optics
- Tumour detection
ASJC Scopus subject areas
- Medicine (miscellaneous)
- Surgery
Cite this
Influence of tumour depth, blood absorption and autofluorescence on measurements of exogenous fluorophores in tissue. / Saarnak, A. E.; Rodrigues, T.; Schwartz, J.; Moore, Ana L; Moore, Thomas A; Gust, John Devens; Van Gemert, M. J C; Sterenborg, H. J C M; Thomsen, S.
In: Lasers in Medical Science, Vol. 13, No. 1, 1998, p. 22-31.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Influence of tumour depth, blood absorption and autofluorescence on measurements of exogenous fluorophores in tissue
AU - Saarnak, A. E.
AU - Rodrigues, T.
AU - Schwartz, J.
AU - Moore, Ana L
AU - Moore, Thomas A
AU - Gust, John Devens
AU - Van Gemert, M. J C
AU - Sterenborg, H. J C M
AU - Thomsen, S.
PY - 1998
Y1 - 1998
N2 - We investigated the influence of tumour depth and differences in blood concentration and autofluorescence between tumour and normal tissue on the fluorescence of a tumour-localising agent. Carotenoporphyrin, CP(Me)3, was injected into rats and nude mice with intradermal tumours. On the tumours an incision was made, uncovering 2 mm2 of the tumour, and fluorescence measurements, including excitation-emission maps and fluorescence ratios, were made on skin, covered and uncovered tumour. The measured fluorescence ratio in the uncovered tumour showed a three- to tenfold increase compared to the covered tumour. We used a one-dimensional layered tissue model to analyse the data. In conclusion, even with a high tumour-selectivity deeper lying tumours cannot always be detected, particularly if the tumour has a high blood concentration or low autofluorescence intensity.
AB - We investigated the influence of tumour depth and differences in blood concentration and autofluorescence between tumour and normal tissue on the fluorescence of a tumour-localising agent. Carotenoporphyrin, CP(Me)3, was injected into rats and nude mice with intradermal tumours. On the tumours an incision was made, uncovering 2 mm2 of the tumour, and fluorescence measurements, including excitation-emission maps and fluorescence ratios, were made on skin, covered and uncovered tumour. The measured fluorescence ratio in the uncovered tumour showed a three- to tenfold increase compared to the covered tumour. We used a one-dimensional layered tissue model to analyse the data. In conclusion, even with a high tumour-selectivity deeper lying tumours cannot always be detected, particularly if the tumour has a high blood concentration or low autofluorescence intensity.
KW - Carothenoporphyrin
KW - Fluorescence ratio
KW - Fluorescence spectroscopy
KW - Tissue optics
KW - Tumour detection
UR - http://www.scopus.com/inward/record.url?scp=0031919056&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031919056&partnerID=8YFLogxK
U2 - 10.1007/BF00592957
DO - 10.1007/BF00592957
M3 - Article
VL - 13
SP - 22
EP - 31
JO - Lasers in Medical Science
JF - Lasers in Medical Science
SN - 0268-8921
IS - 1
ER -