Influence of tumour depth, blood absorption and autofluorescence on measurements of exogenous fluorophores in tissue

A. E. Saarnak; T. Rodrigues; J. Schwartz; Ana L Moore; Thomas A Moore; John Devens Gust; M. J C Van Gemert; H. J C M Sterenborg; S. Thomsen

doi:10.1007/BF00592957

Influence of tumour depth, blood absorption and autofluorescence on measurements of exogenous fluorophores in tissue

A. E. Saarnak, T. Rodrigues, J. Schwartz, Ana L Moore, Thomas A Moore, John Devens Gust, M. J C Van Gemert, H. J C M Sterenborg, S. Thomsen

Arizona State University

Research output: Contribution to journal › Article

11 Citations (Scopus)

Abstract

We investigated the influence of tumour depth and differences in blood concentration and autofluorescence between tumour and normal tissue on the fluorescence of a tumour-localising agent. Carotenoporphyrin, CP(Me)₃, was injected into rats and nude mice with intradermal tumours. On the tumours an incision was made, uncovering 2 mm² of the tumour, and fluorescence measurements, including excitation-emission maps and fluorescence ratios, were made on skin, covered and uncovered tumour. The measured fluorescence ratio in the uncovered tumour showed a three- to tenfold increase compared to the covered tumour. We used a one-dimensional layered tissue model to analyse the data. In conclusion, even with a high tumour-selectivity deeper lying tumours cannot always be detected, particularly if the tumour has a high blood concentration or low autofluorescence intensity.

Original language	English
Pages (from-to)	22-31
Number of pages	10
Journal	Lasers in Medical Science
Volume	13
Issue number	1
DOIs	https://doi.org/10.1007/BF00592957
Publication status	Published - 1998

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Keywords

Carothenoporphyrin
Fluorescence ratio
Fluorescence spectroscopy
Tissue optics
Tumour detection

ASJC Scopus subject areas

Medicine (miscellaneous)
Surgery

Cite this

Saarnak, A. E., Rodrigues, T., Schwartz, J., Moore, A. L., Moore, T. A., Gust, J. D., Van Gemert, M. J. C., Sterenborg, H. J. C. M., & Thomsen, S. (1998). Influence of tumour depth, blood absorption and autofluorescence on measurements of exogenous fluorophores in tissue. Lasers in Medical Science, 13(1), 22-31. https://doi.org/10.1007/BF00592957

Influence of tumour depth, blood absorption and autofluorescence on measurements of exogenous fluorophores in tissue. / Saarnak, A. E.; Rodrigues, T.; Schwartz, J.; Moore, Ana L ; Moore, Thomas A ; Gust, John Devens; Van Gemert, M. J C; Sterenborg, H. J C M; Thomsen, S.

In: Lasers in Medical Science, Vol. 13, No. 1, 1998, p. 22-31.

Research output: Contribution to journal › Article

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abstract = "We investigated the influence of tumour depth and differences in blood concentration and autofluorescence between tumour and normal tissue on the fluorescence of a tumour-localising agent. Carotenoporphyrin, CP(Me)3, was injected into rats and nude mice with intradermal tumours. On the tumours an incision was made, uncovering 2 mm2 of the tumour, and fluorescence measurements, including excitation-emission maps and fluorescence ratios, were made on skin, covered and uncovered tumour. The measured fluorescence ratio in the uncovered tumour showed a three- to tenfold increase compared to the covered tumour. We used a one-dimensional layered tissue model to analyse the data. In conclusion, even with a high tumour-selectivity deeper lying tumours cannot always be detected, particularly if the tumour has a high blood concentration or low autofluorescence intensity.",

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10.1007/BF00592957