Abstract
We investigated the influence of tumour depth and differences in blood concentration and autofluorescence between tumour and normal tissue on the fluorescence of a tumour-localising agent. Carotenoporphyrin, CP(Me)3, was injected into rats and nude mice with intradermal tumours. On the tumours an incision was made, uncovering 2 mm2 of the tumour, and fluorescence measurements, including excitation-emission maps and fluorescence ratios, were made on skin, covered and uncovered tumour. The measured fluorescence ratio in the uncovered tumour showed a three- to tenfold increase compared to the covered tumour. We used a one-dimensional layered tissue model to analyse the data. In conclusion, even with a high tumour-selectivity deeper lying tumours cannot always be detected, particularly if the tumour has a high blood concentration or low autofluorescence intensity.
Original language | English |
---|---|
Pages (from-to) | 22-31 |
Number of pages | 10 |
Journal | Lasers in Medical Science |
Volume | 13 |
Issue number | 1 |
DOIs | |
Publication status | Published - 1998 |
Keywords
- Carothenoporphyrin
- Fluorescence ratio
- Fluorescence spectroscopy
- Tissue optics
- Tumour detection
ASJC Scopus subject areas
- Surgery
- Dermatology