Influence of tumour depth, blood absorption and autofluorescence on measurements of exogenous fluorophores in tissue

A. E. Saarnak, T. Rodrigues, J. Schwartz, Ana L Moore, Thomas A Moore, John Devens Gust, M. J C Van Gemert, H. J C M Sterenborg, S. Thomsen

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11 Citations (Scopus)

Abstract

We investigated the influence of tumour depth and differences in blood concentration and autofluorescence between tumour and normal tissue on the fluorescence of a tumour-localising agent. Carotenoporphyrin, CP(Me)3, was injected into rats and nude mice with intradermal tumours. On the tumours an incision was made, uncovering 2 mm2 of the tumour, and fluorescence measurements, including excitation-emission maps and fluorescence ratios, were made on skin, covered and uncovered tumour. The measured fluorescence ratio in the uncovered tumour showed a three- to tenfold increase compared to the covered tumour. We used a one-dimensional layered tissue model to analyse the data. In conclusion, even with a high tumour-selectivity deeper lying tumours cannot always be detected, particularly if the tumour has a high blood concentration or low autofluorescence intensity.

Original languageEnglish
Pages (from-to)22-31
Number of pages10
JournalLasers in Medical Science
Volume13
Issue number1
DOIs
Publication statusPublished - 1998

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Keywords

  • Carothenoporphyrin
  • Fluorescence ratio
  • Fluorescence spectroscopy
  • Tissue optics
  • Tumour detection

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Surgery

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