Influence of tumour depth, blood absorption and autofluorescence on measurements of exogenous fluorophores in tissue

A. E. Saarnak; T. Rodrigues; J. Schwartz; Ana L Moore; Thomas A Moore; John Devens Gust; M. J C Van Gemert; H. J C M Sterenborg; S. Thomsen

doi:10.1007/BF00592957

Influence of tumour depth, blood absorption and autofluorescence on measurements of exogenous fluorophores in tissue

A. E. Saarnak, T. Rodrigues, J. Schwartz, Ana L Moore, Thomas A Moore, John Devens Gust, M. J C Van Gemert, H. J C M Sterenborg, S. Thomsen

Arizona State University

Research output: Contribution to journal › Article

11 Citations (Scopus)

Abstract

We investigated the influence of tumour depth and differences in blood concentration and autofluorescence between tumour and normal tissue on the fluorescence of a tumour-localising agent. Carotenoporphyrin, CP(Me)₃, was injected into rats and nude mice with intradermal tumours. On the tumours an incision was made, uncovering 2 mm² of the tumour, and fluorescence measurements, including excitation-emission maps and fluorescence ratios, were made on skin, covered and uncovered tumour. The measured fluorescence ratio in the uncovered tumour showed a three- to tenfold increase compared to the covered tumour. We used a one-dimensional layered tissue model to analyse the data. In conclusion, even with a high tumour-selectivity deeper lying tumours cannot always be detected, particularly if the tumour has a high blood concentration or low autofluorescence intensity.

Original language	English
Pages (from-to)	22-31
Number of pages	10
Journal	Lasers in Medical Science
Volume	13
Issue number	1
DOIs	https://doi.org/10.1007/BF00592957
Publication status	Published - 1998

Keywords

Carothenoporphyrin
Fluorescence ratio
Fluorescence spectroscopy
Tissue optics
Tumour detection

ASJC Scopus subject areas

Medicine (miscellaneous)
Surgery

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Saarnak, A. E., Rodrigues, T., Schwartz, J., Moore, A. L., Moore, T. A., Gust, J. D., Van Gemert, M. J. C., Sterenborg, H. J. C. M., & Thomsen, S. (1998). Influence of tumour depth, blood absorption and autofluorescence on measurements of exogenous fluorophores in tissue. Lasers in Medical Science, 13(1), 22-31. https://doi.org/10.1007/BF00592957

Influence of tumour depth, blood absorption and autofluorescence on measurements of exogenous fluorophores in tissue. / Saarnak, A. E.; Rodrigues, T.; Schwartz, J.; Moore, Ana L ; Moore, Thomas A ; Gust, John Devens; Van Gemert, M. J C; Sterenborg, H. J C M; Thomsen, S.

In: Lasers in Medical Science, Vol. 13, No. 1, 1998, p. 22-31.

Research output: Contribution to journal › Article

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abstract = "We investigated the influence of tumour depth and differences in blood concentration and autofluorescence between tumour and normal tissue on the fluorescence of a tumour-localising agent. Carotenoporphyrin, CP(Me)3, was injected into rats and nude mice with intradermal tumours. On the tumours an incision was made, uncovering 2 mm2 of the tumour, and fluorescence measurements, including excitation-emission maps and fluorescence ratios, were made on skin, covered and uncovered tumour. The measured fluorescence ratio in the uncovered tumour showed a three- to tenfold increase compared to the covered tumour. We used a one-dimensional layered tissue model to analyse the data. In conclusion, even with a high tumour-selectivity deeper lying tumours cannot always be detected, particularly if the tumour has a high blood concentration or low autofluorescence intensity.",

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AU - Sterenborg, H. J C M

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