Inhibition of the catalase reaction of Photosystem II by anions

Junichi Mano, Kunio Kawamoto, G Charles Dismukes, Kozi Asada

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A new binding site for anions which inhibit the water oxidizing complex (WOC) of Photosystem II in spinach has been identified. Anions which bind to this site inhibit the flash-induced S2/S0 catalase reaction (2H2O2→2H2O+O2) of the WOC by displacing hydrogen peroxide. Using a mass spectrometer and gas permeable membrane to detect the 32O2 product, the yield and lifetime of the active state of the flash-induced catalase (to be referred to simply as 'flash-catalase') reaction were measured after forming the S2 or S0-states by a short flash. The increase in flash-catalase activity with H2O2 concentration exhibits a Km=10-20 mM, and originates from an increase in the lifetime by 20-fold of the active state. The increased lifetime in the presence of peroxide is ascribed to formation of the long-lived S0-state at the expense of the unstable S2-state. The anion inhibition site differs from the chloride site involved in stimulating the photolytic water oxidation reaction (2H2O→O2+4e-+4H+). Whereas water oxidation requires Cl- and is inhibited with increasing effectiveness by F-≪CN-≪N3-, the flash-catalase reaction is weakly inhibited by Cl-, and with increasing effectiveness by F-≪CN-, N3-. Unlike water oxidation, chloride is unable to suppress or reverse inhibition of the flash-catalase reaction caused by these anions. The inhibitor effectiveness correlates with the pKa of the conjugate acid, suggesting that the protonated species may be the active inhibitor. The reduced activity arises from a shortening of the lifetime of the flash-induced catalase active state by 3-10 fold owing to stronger anion binding in the flash-induced states, S2 and S0, than in the dark S-states, S1 and S-1. To account for the paradoxical result that higher anion concentrations are required to inhibit at lower H2O2 concentrations, where S2 forms initially after the flash, than at higher H2O2 concentrations, where S0 forms initially after the flash, stronger anion binding to the S0-state than to the S2-state is proposed. A kinetic model is given which accounts for these equilibria with anions and H2O2. The rate constant for the formation/release of O2 by reduction of S2 in the WOC is -1.

Original languageEnglish
Pages (from-to)433-440
Number of pages8
JournalPhotosynthesis Research
Volume38
Issue number3
DOIs
Publication statusPublished - Jan 1993

Fingerprint

Photosystem II Protein Complex
anions
Catalase
photosystem II
Anions
catalase
Water
water
oxidation
Oxidation
Chlorides
Gas permeable membranes
chlorides
Spinacia oleracea
Peroxides
Mass spectrometers
spectrometers
peroxides
spinach
shortenings

Keywords

  • enzyme
  • inhibitors
  • manganese
  • oxygen evolution
  • peroxide
  • water oxidation

ASJC Scopus subject areas

  • Plant Science

Cite this

Inhibition of the catalase reaction of Photosystem II by anions. / Mano, Junichi; Kawamoto, Kunio; Dismukes, G Charles; Asada, Kozi.

In: Photosynthesis Research, Vol. 38, No. 3, 01.1993, p. 433-440.

Research output: Contribution to journalArticle

Mano, Junichi ; Kawamoto, Kunio ; Dismukes, G Charles ; Asada, Kozi. / Inhibition of the catalase reaction of Photosystem II by anions. In: Photosynthesis Research. 1993 ; Vol. 38, No. 3. pp. 433-440.
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N2 - A new binding site for anions which inhibit the water oxidizing complex (WOC) of Photosystem II in spinach has been identified. Anions which bind to this site inhibit the flash-induced S2/S0 catalase reaction (2H2O2→2H2O+O2) of the WOC by displacing hydrogen peroxide. Using a mass spectrometer and gas permeable membrane to detect the 32O2 product, the yield and lifetime of the active state of the flash-induced catalase (to be referred to simply as 'flash-catalase') reaction were measured after forming the S2 or S0-states by a short flash. The increase in flash-catalase activity with H2O2 concentration exhibits a Km=10-20 mM, and originates from an increase in the lifetime by 20-fold of the active state. The increased lifetime in the presence of peroxide is ascribed to formation of the long-lived S0-state at the expense of the unstable S2-state. The anion inhibition site differs from the chloride site involved in stimulating the photolytic water oxidation reaction (2H2O→O2+4e-+4H+). Whereas water oxidation requires Cl- and is inhibited with increasing effectiveness by F-≪CN-≪N3-, the flash-catalase reaction is weakly inhibited by Cl-, and with increasing effectiveness by F-≪CN-, N3-. Unlike water oxidation, chloride is unable to suppress or reverse inhibition of the flash-catalase reaction caused by these anions. The inhibitor effectiveness correlates with the pKa of the conjugate acid, suggesting that the protonated species may be the active inhibitor. The reduced activity arises from a shortening of the lifetime of the flash-induced catalase active state by 3-10 fold owing to stronger anion binding in the flash-induced states, S2 and S0, than in the dark S-states, S1 and S-1. To account for the paradoxical result that higher anion concentrations are required to inhibit at lower H2O2 concentrations, where S2 forms initially after the flash, than at higher H2O2 concentrations, where S0 forms initially after the flash, stronger anion binding to the S0-state than to the S2-state is proposed. A kinetic model is given which accounts for these equilibria with anions and H2O2. The rate constant for the formation/release of O2 by reduction of S2 in the WOC is -1.

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