Instructing cells with programmable peptide DNA hybrids

Ronit Freeman, Nicholas Stephanopoulos, Zaida Álvarez, Jacob A. Lewis, Shantanu Sur, Chris M. Serrano, Job Boekhoven, Sungsoo S. Lee, Samuel I Stupp

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The native extracellular matrix is a space in which signals can be displayed dynamically and reversibly, positioned with nanoscale precision, and combined synergistically to control cell function. Here we describe a molecular system that can be programmed to control these three characteristics. In this approach we immobilize peptide-DNA (P-DNA) molecules on a surface through complementary DNA tethers directing cells to adhere and spread reversibly over multiple cycles. The DNA can also serve as a molecular ruler to control the distance-dependent synergy between two peptides. Finally, we use two orthogonal DNA handles to regulate two different bioactive signals, with the ability to independently up- or downregulate each over time. This enabled us to discover that neural stem cells, derived from the murine spinal cord and organized as neurospheres, can be triggered to migrate out in response to an exogenous signal but then regroup into a neurosphere as the signal is removed.

Original languageEnglish
Article number15982
JournalNature Communications
Volume8
DOIs
Publication statusPublished - Jul 10 2017

Fingerprint

peptides
deoxyribonucleic acid
Peptides
DNA
cells
Neural Stem Cells
complementary DNA
Stem cells
spinal cord
Extracellular Matrix
stem cells
Spinal Cord
Up-Regulation
Down-Regulation
Complementary DNA
activity (biology)
Molecules
cycles
matrices
molecules

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Freeman, R., Stephanopoulos, N., Álvarez, Z., Lewis, J. A., Sur, S., Serrano, C. M., ... Stupp, S. I. (2017). Instructing cells with programmable peptide DNA hybrids. Nature Communications, 8, [15982]. https://doi.org/10.1038/ncomms15982

Instructing cells with programmable peptide DNA hybrids. / Freeman, Ronit; Stephanopoulos, Nicholas; Álvarez, Zaida; Lewis, Jacob A.; Sur, Shantanu; Serrano, Chris M.; Boekhoven, Job; Lee, Sungsoo S.; Stupp, Samuel I.

In: Nature Communications, Vol. 8, 15982, 10.07.2017.

Research output: Contribution to journalArticle

Freeman, R, Stephanopoulos, N, Álvarez, Z, Lewis, JA, Sur, S, Serrano, CM, Boekhoven, J, Lee, SS & Stupp, SI 2017, 'Instructing cells with programmable peptide DNA hybrids', Nature Communications, vol. 8, 15982. https://doi.org/10.1038/ncomms15982
Freeman R, Stephanopoulos N, Álvarez Z, Lewis JA, Sur S, Serrano CM et al. Instructing cells with programmable peptide DNA hybrids. Nature Communications. 2017 Jul 10;8. 15982. https://doi.org/10.1038/ncomms15982
Freeman, Ronit ; Stephanopoulos, Nicholas ; Álvarez, Zaida ; Lewis, Jacob A. ; Sur, Shantanu ; Serrano, Chris M. ; Boekhoven, Job ; Lee, Sungsoo S. ; Stupp, Samuel I. / Instructing cells with programmable peptide DNA hybrids. In: Nature Communications. 2017 ; Vol. 8.
@article{6e361bcbbcb54de991f8a5f5b18f805a,
title = "Instructing cells with programmable peptide DNA hybrids",
abstract = "The native extracellular matrix is a space in which signals can be displayed dynamically and reversibly, positioned with nanoscale precision, and combined synergistically to control cell function. Here we describe a molecular system that can be programmed to control these three characteristics. In this approach we immobilize peptide-DNA (P-DNA) molecules on a surface through complementary DNA tethers directing cells to adhere and spread reversibly over multiple cycles. The DNA can also serve as a molecular ruler to control the distance-dependent synergy between two peptides. Finally, we use two orthogonal DNA handles to regulate two different bioactive signals, with the ability to independently up- or downregulate each over time. This enabled us to discover that neural stem cells, derived from the murine spinal cord and organized as neurospheres, can be triggered to migrate out in response to an exogenous signal but then regroup into a neurosphere as the signal is removed.",
author = "Ronit Freeman and Nicholas Stephanopoulos and Zaida {\'A}lvarez and Lewis, {Jacob A.} and Shantanu Sur and Serrano, {Chris M.} and Job Boekhoven and Lee, {Sungsoo S.} and Stupp, {Samuel I}",
year = "2017",
month = "7",
day = "10",
doi = "10.1038/ncomms15982",
language = "English",
volume = "8",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - Instructing cells with programmable peptide DNA hybrids

AU - Freeman, Ronit

AU - Stephanopoulos, Nicholas

AU - Álvarez, Zaida

AU - Lewis, Jacob A.

AU - Sur, Shantanu

AU - Serrano, Chris M.

AU - Boekhoven, Job

AU - Lee, Sungsoo S.

AU - Stupp, Samuel I

PY - 2017/7/10

Y1 - 2017/7/10

N2 - The native extracellular matrix is a space in which signals can be displayed dynamically and reversibly, positioned with nanoscale precision, and combined synergistically to control cell function. Here we describe a molecular system that can be programmed to control these three characteristics. In this approach we immobilize peptide-DNA (P-DNA) molecules on a surface through complementary DNA tethers directing cells to adhere and spread reversibly over multiple cycles. The DNA can also serve as a molecular ruler to control the distance-dependent synergy between two peptides. Finally, we use two orthogonal DNA handles to regulate two different bioactive signals, with the ability to independently up- or downregulate each over time. This enabled us to discover that neural stem cells, derived from the murine spinal cord and organized as neurospheres, can be triggered to migrate out in response to an exogenous signal but then regroup into a neurosphere as the signal is removed.

AB - The native extracellular matrix is a space in which signals can be displayed dynamically and reversibly, positioned with nanoscale precision, and combined synergistically to control cell function. Here we describe a molecular system that can be programmed to control these three characteristics. In this approach we immobilize peptide-DNA (P-DNA) molecules on a surface through complementary DNA tethers directing cells to adhere and spread reversibly over multiple cycles. The DNA can also serve as a molecular ruler to control the distance-dependent synergy between two peptides. Finally, we use two orthogonal DNA handles to regulate two different bioactive signals, with the ability to independently up- or downregulate each over time. This enabled us to discover that neural stem cells, derived from the murine spinal cord and organized as neurospheres, can be triggered to migrate out in response to an exogenous signal but then regroup into a neurosphere as the signal is removed.

UR - http://www.scopus.com/inward/record.url?scp=85023165913&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85023165913&partnerID=8YFLogxK

U2 - 10.1038/ncomms15982

DO - 10.1038/ncomms15982

M3 - Article

VL - 8

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 15982

ER -