Interenzyme substrate diffusion for an enzyme cascade organized on spatially addressable DNA nanostructures

Jinglin Fu, Minghui Liu, Yan Liu, Neal W. Woodbury, Hao Yan

Research output: Contribution to journalArticle

323 Citations (Scopus)

Abstract

Spatially addressable DNA nanostructures facilitate the self-assembly of heterogeneous elements with precisely controlled patterns. Here we organized discrete glucose oxidase (GOx)/horseradish peroxidase (HRP) enzyme pairs on specific DNA origami tiles with controlled interenzyme spacing and position. The distance between enzymes was systematically varied from 10 to 65 nm, and the corresponding activities were evaluated. The study revealed two different distance-dependent kinetic processes associated with the assembled enzyme pairs. Strongly enhanced activity was observed for those assemblies in which the enzymes were closely spaced, while the activity dropped dramatically for enzymes as little as 20 nm apart. Increasing the spacing further resulted in a much weaker distance dependence. Combined with diffusion modeling, the results suggest that Brownian diffusion of intermediates in solution governed the variations in activity for more distant enzyme pairs, while dimensionally limited diffusion of intermediates across connected protein surfaces contributed to the enhancement in activity for closely spaced GOx/HRP assemblies. To further test the role of limited dimensional diffusion along protein surfaces, a noncatalytic protein bridge was inserted between GOx and HRP to connect their hydration shells. This resulted in substantially enhanced activity of the enzyme pair.

Original languageEnglish
Pages (from-to)5516-5519
Number of pages4
JournalJournal of the American Chemical Society
Volume134
Issue number12
DOIs
Publication statusPublished - Mar 28 2012

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Nanostructures
DNA
Enzymes
Glucose Oxidase
Substrates
Glucose oxidase
Horseradish Peroxidase
Proteins
Membrane Proteins
Enzyme kinetics
Tile
Hydration
Self assembly
Catalyst activity
Kinetics

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Interenzyme substrate diffusion for an enzyme cascade organized on spatially addressable DNA nanostructures. / Fu, Jinglin; Liu, Minghui; Liu, Yan; Woodbury, Neal W.; Yan, Hao.

In: Journal of the American Chemical Society, Vol. 134, No. 12, 28.03.2012, p. 5516-5519.

Research output: Contribution to journalArticle

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