Abstract
We investigated the engineered bioconjugate of cadmium selenide core/zinc sulfide shell, (CdSe)ZnS, quantum dots (QDs) with genetically modified proteins using near-field scanning optical microscopy (NSOM). A genetically engineered protein polymer was expressed and purified from E. coli. The protein polymer was allowed to serf-assemble to the bacterial microcrystalline cellulose surface through the cellulosic binding domain. QDs were then conjugated to the protein/cellulose assembly through interaction with the oxhistidine tag on the protein. The transmitted near-field optical signals are collected and detected by both a PMT (near-field scanning optical microscopy, NSOM) and a spectrometer (near-field scanning optical spectroscopy, NSOS). Results from the sample containing the QDs/protein/cellulose assemblies suggest that QDs were arrayed along the cellulose surface. The near-field spectroscopic study also showed that the slight change of spectroscopic properties of the QDs upon bioconjugation, indicating the strong interaction between the constructed protein and QDs.
Original language | English |
---|---|
Article number | A-41 |
Pages (from-to) | 137-144 |
Number of pages | 8 |
Journal | Proceedings of SPIE - The International Society for Optical Engineering |
Volume | 5513 |
DOIs | |
Publication status | Published - Dec 1 2004 |
Event | Physical Chemistry of Interfaces and Nanomaterials III - Denver, CO, United States Duration: Aug 3 2004 → Aug 6 2004 |
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Computer Science Applications
- Applied Mathematics
- Electrical and Electronic Engineering