Abstract
We investigated the engineered bioconjugate of cadmium selenide core/zinc sulfide shell, (CdSe)ZnS, quantum dots (QDs) with genetically modified proteins using near-field scanning optical microscopy (NSOM). A genetically engineered protein polymer was expressed and purified from E. coli. The protein polymer was allowed to serf-assemble to the bacterial microcrystalline cellulose surface through the cellulosic binding domain. QDs were then conjugated to the protein/cellulose assembly through interaction with the oxhistidine tag on the protein. The transmitted near-field optical signals are collected and detected by both a PMT (near-field scanning optical microscopy, NSOM) and a spectrometer (near-field scanning optical spectroscopy, NSOS). Results from the sample containing the QDs/protein/cellulose assemblies suggest that QDs were arrayed along the cellulose surface. The near-field spectroscopic study also showed that the slight change of spectroscopic properties of the QDs upon bioconjugation, indicating the strong interaction between the constructed protein and QDs.
| Original language | English |
|---|---|
| Title of host publication | Proceedings of SPIE - The International Society for Optical Engineering |
| Editors | G.V. Hartland, X.-Y. Zhu |
| Pages | 137-144 |
| Number of pages | 8 |
| Volume | 5513 |
| DOIs | |
| Publication status | Published - 2004 |
| Event | Physical Chemistry of Interfaces and Nanomaterials III - Denver, CO, United States Duration: Aug 3 2004 → Aug 6 2004 |
Other
| Other | Physical Chemistry of Interfaces and Nanomaterials III |
|---|---|
| Country | United States |
| City | Denver, CO |
| Period | 8/3/04 → 8/6/04 |
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ASJC Scopus subject areas
- Electrical and Electronic Engineering
- Condensed Matter Physics
Cite this
Investigation of novel quantum dots/proteins/cellulose bioconjugate using NSOM and photoluminescence. / Zhang, Peng; Ding, Shi You; Xu, Qi; Smith, Steve; Rumbles, Gary; Himmel, Michael E.
Proceedings of SPIE - The International Society for Optical Engineering. ed. / G.V. Hartland; X.-Y. Zhu. Vol. 5513 2004. p. 137-144 A-41.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Investigation of novel quantum dots/proteins/cellulose bioconjugate using NSOM and photoluminescence
AU - Zhang, Peng
AU - Ding, Shi You
AU - Xu, Qi
AU - Smith, Steve
AU - Rumbles, Gary
AU - Himmel, Michael E.
PY - 2004
Y1 - 2004
N2 - We investigated the engineered bioconjugate of cadmium selenide core/zinc sulfide shell, (CdSe)ZnS, quantum dots (QDs) with genetically modified proteins using near-field scanning optical microscopy (NSOM). A genetically engineered protein polymer was expressed and purified from E. coli. The protein polymer was allowed to serf-assemble to the bacterial microcrystalline cellulose surface through the cellulosic binding domain. QDs were then conjugated to the protein/cellulose assembly through interaction with the oxhistidine tag on the protein. The transmitted near-field optical signals are collected and detected by both a PMT (near-field scanning optical microscopy, NSOM) and a spectrometer (near-field scanning optical spectroscopy, NSOS). Results from the sample containing the QDs/protein/cellulose assemblies suggest that QDs were arrayed along the cellulose surface. The near-field spectroscopic study also showed that the slight change of spectroscopic properties of the QDs upon bioconjugation, indicating the strong interaction between the constructed protein and QDs.
AB - We investigated the engineered bioconjugate of cadmium selenide core/zinc sulfide shell, (CdSe)ZnS, quantum dots (QDs) with genetically modified proteins using near-field scanning optical microscopy (NSOM). A genetically engineered protein polymer was expressed and purified from E. coli. The protein polymer was allowed to serf-assemble to the bacterial microcrystalline cellulose surface through the cellulosic binding domain. QDs were then conjugated to the protein/cellulose assembly through interaction with the oxhistidine tag on the protein. The transmitted near-field optical signals are collected and detected by both a PMT (near-field scanning optical microscopy, NSOM) and a spectrometer (near-field scanning optical spectroscopy, NSOS). Results from the sample containing the QDs/protein/cellulose assemblies suggest that QDs were arrayed along the cellulose surface. The near-field spectroscopic study also showed that the slight change of spectroscopic properties of the QDs upon bioconjugation, indicating the strong interaction between the constructed protein and QDs.
UR - http://www.scopus.com/inward/record.url?scp=13244292233&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=13244292233&partnerID=8YFLogxK
U2 - 10.1117/12.560231
DO - 10.1117/12.560231
M3 - Conference contribution
VL - 5513
SP - 137
EP - 144
BT - Proceedings of SPIE - The International Society for Optical Engineering
A2 - Hartland, G.V.
A2 - Zhu, X.-Y.
ER -