Investigation of novel quantum dots/proteins/cellulose bioconjugate using NSOM and photoluminescence

Peng Zhang, Shi You Ding, Qi Xu, Steve Smith, Gary Rumbles, Michael E. Himmel

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We investigated the engineered bioconjugate of cadmium selenide core/zinc sulfide shell, (CdSe)ZnS, quantum dots (QDs) with genetically modified proteins using near-field scanning optical microscopy (NSOM). A genetically engineered protein polymer was expressed and purified from E. coli. The protein polymer was allowed to serf-assemble to the bacterial microcrystalline cellulose surface through the cellulosic binding domain. QDs were then conjugated to the protein/cellulose assembly through interaction with the oxhistidine tag on the protein. The transmitted near-field optical signals are collected and detected by both a PMT (near-field scanning optical microscopy, NSOM) and a spectrometer (near-field scanning optical spectroscopy, NSOS). Results from the sample containing the QDs/protein/cellulose assemblies suggest that QDs were arrayed along the cellulose surface. The near-field spectroscopic study also showed that the slight change of spectroscopic properties of the QDs upon bioconjugation, indicating the strong interaction between the constructed protein and QDs.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsG.V. Hartland, X.-Y. Zhu
Pages137-144
Number of pages8
Volume5513
DOIs
Publication statusPublished - 2004
EventPhysical Chemistry of Interfaces and Nanomaterials III - Denver, CO, United States
Duration: Aug 3 2004Aug 6 2004

Other

OtherPhysical Chemistry of Interfaces and Nanomaterials III
CountryUnited States
CityDenver, CO
Period8/3/048/6/04

Fingerprint

Near field scanning optical microscopy
cellulose
Semiconductor quantum dots
Cellulose
near fields
Photoluminescence
quantum dots
microscopy
proteins
Proteins
photoluminescence
scanning
cadmium selenides
Zinc sulfide
zinc sulfides
polymers
Polymers
Cadmium
Escherichia coli
assemblies

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Zhang, P., Ding, S. Y., Xu, Q., Smith, S., Rumbles, G., & Himmel, M. E. (2004). Investigation of novel quantum dots/proteins/cellulose bioconjugate using NSOM and photoluminescence. In G. V. Hartland, & X-Y. Zhu (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 5513, pp. 137-144). [A-41] https://doi.org/10.1117/12.560231

Investigation of novel quantum dots/proteins/cellulose bioconjugate using NSOM and photoluminescence. / Zhang, Peng; Ding, Shi You; Xu, Qi; Smith, Steve; Rumbles, Gary; Himmel, Michael E.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / G.V. Hartland; X.-Y. Zhu. Vol. 5513 2004. p. 137-144 A-41.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Zhang, P, Ding, SY, Xu, Q, Smith, S, Rumbles, G & Himmel, ME 2004, Investigation of novel quantum dots/proteins/cellulose bioconjugate using NSOM and photoluminescence. in GV Hartland & X-Y Zhu (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 5513, A-41, pp. 137-144, Physical Chemistry of Interfaces and Nanomaterials III, Denver, CO, United States, 8/3/04. https://doi.org/10.1117/12.560231
Zhang P, Ding SY, Xu Q, Smith S, Rumbles G, Himmel ME. Investigation of novel quantum dots/proteins/cellulose bioconjugate using NSOM and photoluminescence. In Hartland GV, Zhu X-Y, editors, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 5513. 2004. p. 137-144. A-41 https://doi.org/10.1117/12.560231
Zhang, Peng ; Ding, Shi You ; Xu, Qi ; Smith, Steve ; Rumbles, Gary ; Himmel, Michael E. / Investigation of novel quantum dots/proteins/cellulose bioconjugate using NSOM and photoluminescence. Proceedings of SPIE - The International Society for Optical Engineering. editor / G.V. Hartland ; X.-Y. Zhu. Vol. 5513 2004. pp. 137-144
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