Investigation of novel quantum dots/proteins/cellulose bioconjugate using NSOM and photoluminescence

Peng Zhang; Shi You Ding; Qi Xu; Steve Smith; Garry Rumbles; Michael E. Himmel

doi:10.1117/12.560231

Investigation of novel quantum dots/proteins/cellulose bioconjugate using NSOM and photoluminescence

Peng Zhang, Shi You Ding, Qi Xu, Steve Smith, Garry Rumbles, Michael E. Himmel

National Renewable Energy Laboratory

Research output: Contribution to journal › Conference article

Abstract

We investigated the engineered bioconjugate of cadmium selenide core/zinc sulfide shell, (CdSe)ZnS, quantum dots (QDs) with genetically modified proteins using near-field scanning optical microscopy (NSOM). A genetically engineered protein polymer was expressed and purified from E. coli. The protein polymer was allowed to serf-assemble to the bacterial microcrystalline cellulose surface through the cellulosic binding domain. QDs were then conjugated to the protein/cellulose assembly through interaction with the oxhistidine tag on the protein. The transmitted near-field optical signals are collected and detected by both a PMT (near-field scanning optical microscopy, NSOM) and a spectrometer (near-field scanning optical spectroscopy, NSOS). Results from the sample containing the QDs/protein/cellulose assemblies suggest that QDs were arrayed along the cellulose surface. The near-field spectroscopic study also showed that the slight change of spectroscopic properties of the QDs upon bioconjugation, indicating the strong interaction between the constructed protein and QDs.

Original language	English
Article number	A-41
Pages (from-to)	137-144
Number of pages	8
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	5513
DOIs	https://doi.org/10.1117/12.560231
Publication status	Published - Dec 1 2004
Event	Physical Chemistry of Interfaces and Nanomaterials III - Denver, CO, United States Duration: Aug 3 2004 → Aug 6 2004

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

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Zhang, P., Ding, S. Y., Xu, Q., Smith, S., Rumbles, G., & Himmel, M. E. (2004). Investigation of novel quantum dots/proteins/cellulose bioconjugate using NSOM and photoluminescence. Proceedings of SPIE - The International Society for Optical Engineering, 5513, 137-144. [A-41]. https://doi.org/10.1117/12.560231

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abstract = "We investigated the engineered bioconjugate of cadmium selenide core/zinc sulfide shell, (CdSe)ZnS, quantum dots (QDs) with genetically modified proteins using near-field scanning optical microscopy (NSOM). A genetically engineered protein polymer was expressed and purified from E. coli. The protein polymer was allowed to serf-assemble to the bacterial microcrystalline cellulose surface through the cellulosic binding domain. QDs were then conjugated to the protein/cellulose assembly through interaction with the oxhistidine tag on the protein. The transmitted near-field optical signals are collected and detected by both a PMT (near-field scanning optical microscopy, NSOM) and a spectrometer (near-field scanning optical spectroscopy, NSOS). Results from the sample containing the QDs/protein/cellulose assemblies suggest that QDs were arrayed along the cellulose surface. The near-field spectroscopic study also showed that the slight change of spectroscopic properties of the QDs upon bioconjugation, indicating the strong interaction between the constructed protein and QDs.",

author = "Peng Zhang and Ding, {Shi You} and Qi Xu and Steve Smith and Garry Rumbles and Himmel, {Michael E.}",

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AU - Zhang, Peng

AU - Ding, Shi You

AU - Xu, Qi

AU - Smith, Steve

AU - Rumbles, Garry

AU - Himmel, Michael E.

PY - 2004/12/1

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N2 - We investigated the engineered bioconjugate of cadmium selenide core/zinc sulfide shell, (CdSe)ZnS, quantum dots (QDs) with genetically modified proteins using near-field scanning optical microscopy (NSOM). A genetically engineered protein polymer was expressed and purified from E. coli. The protein polymer was allowed to serf-assemble to the bacterial microcrystalline cellulose surface through the cellulosic binding domain. QDs were then conjugated to the protein/cellulose assembly through interaction with the oxhistidine tag on the protein. The transmitted near-field optical signals are collected and detected by both a PMT (near-field scanning optical microscopy, NSOM) and a spectrometer (near-field scanning optical spectroscopy, NSOS). Results from the sample containing the QDs/protein/cellulose assemblies suggest that QDs were arrayed along the cellulose surface. The near-field spectroscopic study also showed that the slight change of spectroscopic properties of the QDs upon bioconjugation, indicating the strong interaction between the constructed protein and QDs.

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