Kinetics of a collagen-like polypeptide fragmentation after mid-IR free-electron laser ablation

Andrey Zavalin, David L. Hachey, Munirathinam Sundaramoorthy, Surajit Banerjee, Steven Morgan, Leonard C Feldman, Norman Tolk, David W. Piston

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Tissue ablation with mid-infrared irradiation tuned to collagen vibrational modes results in minimal collateral damage. The hypothesis for this effect includes selective scission of protein molecules and excitation of surrounding water molecules, with the scission process currently favored. In this article, we describe the postablation infrared spectral decay kinetics in a model collagen-like peptide (Pro-Pro-Gly)10. We find that the decay is exponential with different decay times for other, simpler dipeptides. Furthermore, we find that collagen-like polypeptides, such as (Pro-Pro-Gly) 10, show multiple decay times, indicating multiple scission locations and cross-linking to form longer chain molecules. In combination with data from high-resolution mass spectrometry, we interpret these products to result from the generation of reactive intermediates, such as free radicals, cyanate ions, and isocyanic acid, which can form cross-links and protein adducts. Our results lead to a more complete explanation of the reduced collateral damage resulting from infrared laser irradiation through a mechanism involving cross-linking in which collagen-like molecules form a network of cross-linked fibers.

Original languageEnglish
Pages (from-to)1371-1381
Number of pages11
JournalBiophysical Journal
Volume95
Issue number3
DOIs
Publication statusPublished - Aug 1 2008

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Laser Therapy
Collagen
Electrons
Peptides
Cyanates
Dipeptides
Free Radicals
Mass Spectrometry
Lasers
Ions
Water
Proteins
(prolyl-prolyl-glycine)10

ASJC Scopus subject areas

  • Biophysics

Cite this

Zavalin, A., Hachey, D. L., Sundaramoorthy, M., Banerjee, S., Morgan, S., Feldman, L. C., ... Piston, D. W. (2008). Kinetics of a collagen-like polypeptide fragmentation after mid-IR free-electron laser ablation. Biophysical Journal, 95(3), 1371-1381. https://doi.org/10.1529/biophysj.107.122002

Kinetics of a collagen-like polypeptide fragmentation after mid-IR free-electron laser ablation. / Zavalin, Andrey; Hachey, David L.; Sundaramoorthy, Munirathinam; Banerjee, Surajit; Morgan, Steven; Feldman, Leonard C; Tolk, Norman; Piston, David W.

In: Biophysical Journal, Vol. 95, No. 3, 01.08.2008, p. 1371-1381.

Research output: Contribution to journalArticle

Zavalin, A, Hachey, DL, Sundaramoorthy, M, Banerjee, S, Morgan, S, Feldman, LC, Tolk, N & Piston, DW 2008, 'Kinetics of a collagen-like polypeptide fragmentation after mid-IR free-electron laser ablation', Biophysical Journal, vol. 95, no. 3, pp. 1371-1381. https://doi.org/10.1529/biophysj.107.122002
Zavalin, Andrey ; Hachey, David L. ; Sundaramoorthy, Munirathinam ; Banerjee, Surajit ; Morgan, Steven ; Feldman, Leonard C ; Tolk, Norman ; Piston, David W. / Kinetics of a collagen-like polypeptide fragmentation after mid-IR free-electron laser ablation. In: Biophysical Journal. 2008 ; Vol. 95, No. 3. pp. 1371-1381.
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