The kinetics of proton-transport-coupled ATP synthesis catalyzed by the chloroplast ATPase (ATP synthase) was investigated after energization of the membrane by an artificially generated DELTA pH and an electric potential difference, DELTA psi . With a rapid mixing system, ATP synthesis was studied at short reaction times ( less than 200 ms) where all relevant parameters (pH//o//u//t, pH//i//n, DELTA psi , substrate and product concentrations) remain practically constant at their initial values. The reaction is investigated at three levels of complexity. (1) The ATPase is embedded in the natural membrane. (2) The ATPase is isolated, purified and reconstituted into asolectin liposomes. (3) The reconstituted ATPase is investigated under single site, single turnover conditions.
|Number of pages||7|
|Journal||Berichte der Bunsengesellschaft/Physical Chemistry Chemical Physics|
|Publication status||Published - Nov 1986|
ASJC Scopus subject areas
- Chemical Engineering(all)