Laser-induced fluorescence in malignant and normal tissue in mice injected with two different carotenoporphyrins

H. Nilsson, J. Johansson, K. Svanberg, S. Svanberg, G. Jori, E. Reddi, A. Segalla, D. Gust, A. L. Moore, T. A. Moore

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Laser-induced fluorescence (LIF) was used to characterise the localisation of an intravenously administered trimethylated carotenoporphyrin [CP(Me)3] and a trimethoxylated carotenoporphyrin [CP(OMe)3] in an intramuscularly transplanted malignant tumour (MS-2 fibrosarcoma) and healthy muscle in female Balb/c mice, 3, 24, 48 and 96 h post injection. The fluorescence was induced with a dye laser pumped by a nitrogen laser, emitting light at 425 nm. The fluorescence spectra were recorded in the region 455-760 nm using a polychromator equipped with an image-intensified CCD camera. The tumour/peritumoral muscle ratio was about 5:1 for CP(Me)3 and about 6:1 for CP(OMe)3 in terms of the background-free fluorescence intensity, which peaked at about 655 nm. By including the endogenous tissue fluorescence, the contrast was further enhanced by a factor of approximately 2.

Original languageEnglish
Pages (from-to)873-879
Number of pages7
JournalBritish Journal of Cancer
Issue number5
Publication statusPublished - Nov 1994


ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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