Laser induced fluorescence studies of the biodistribution of carotenoporphyrins in mice

H. Nilsson, J. Johansson, K. Svanberg, S. Svanberg, G. Jori, E. Reddi, A. Segalla, John Devens Gust, Ana L Moore, Thomas A Moore

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The biodistribution of two recently developed tumour markers, trimethylated (CP(Me)3) and trimethoxylated (CP(OMe)3) carotenoporphyrin, was investigated by means of laser-induced fluorescence (LIF) after i.v. injection into 38 tumour-bearing (MS-2 fibrosarcoma) female Balb/c mice. At 3, 24, 48 or 96 h after administration, the carotenoporphyrin fluorescence was measured in tumoral and peritumoral tissue, as well as in the abdominal, thoracic and cranial cavities. The fluorescence was induced by a nitrogen laser-pumped dye laser, emitting light at 425 nm, and analysed by a polychromator equipped with an image-intensified CCD camera. The fluorescence was evaluated at 490, 655 and 720 nm: the second and third wavelengths represent the carotenoporphyrin (CP)-related peaks, whereas the first one is close to the peak of the tissue autofluorescence. The tumour and the liver were the two tissue types showing the strongest carotenoporphyrin-related fluorescence, whereas the cerebral cortex and muscle consistently exhibited weak substance-related fluorescence. In most tissue types, the fluorescence intensities decreased over time. A few exceptions were observed, notably the liver, in which the intensity remained remarkably constant over the time period investigated.

Original languageEnglish
Pages (from-to)355-364
Number of pages10
JournalBritish Journal of Cancer
Volume76
Issue number3
Publication statusPublished - 1997

Fingerprint

Lasers
Fluorescence
Thoracic Cavity
Dye Lasers
Gas Lasers
Fibrosarcoma
Liver
Tumor Biomarkers
Cerebral Cortex
Neoplasms
Light
Muscles
Injections

Keywords

  • Biodistribution
  • Carotenoporphyrin
  • Laser-induced fluorescence
  • Tumour detection

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Nilsson, H., Johansson, J., Svanberg, K., Svanberg, S., Jori, G., Reddi, E., ... Moore, T. A. (1997). Laser induced fluorescence studies of the biodistribution of carotenoporphyrins in mice. British Journal of Cancer, 76(3), 355-364.

Laser induced fluorescence studies of the biodistribution of carotenoporphyrins in mice. / Nilsson, H.; Johansson, J.; Svanberg, K.; Svanberg, S.; Jori, G.; Reddi, E.; Segalla, A.; Gust, John Devens; Moore, Ana L; Moore, Thomas A.

In: British Journal of Cancer, Vol. 76, No. 3, 1997, p. 355-364.

Research output: Contribution to journalArticle

Nilsson, H, Johansson, J, Svanberg, K, Svanberg, S, Jori, G, Reddi, E, Segalla, A, Gust, JD, Moore, AL & Moore, TA 1997, 'Laser induced fluorescence studies of the biodistribution of carotenoporphyrins in mice', British Journal of Cancer, vol. 76, no. 3, pp. 355-364.
Nilsson H, Johansson J, Svanberg K, Svanberg S, Jori G, Reddi E et al. Laser induced fluorescence studies of the biodistribution of carotenoporphyrins in mice. British Journal of Cancer. 1997;76(3):355-364.
Nilsson, H. ; Johansson, J. ; Svanberg, K. ; Svanberg, S. ; Jori, G. ; Reddi, E. ; Segalla, A. ; Gust, John Devens ; Moore, Ana L ; Moore, Thomas A. / Laser induced fluorescence studies of the biodistribution of carotenoporphyrins in mice. In: British Journal of Cancer. 1997 ; Vol. 76, No. 3. pp. 355-364.
@article{e10f72b090d04bf5b4b6d01165972ccb,
title = "Laser induced fluorescence studies of the biodistribution of carotenoporphyrins in mice",
abstract = "The biodistribution of two recently developed tumour markers, trimethylated (CP(Me)3) and trimethoxylated (CP(OMe)3) carotenoporphyrin, was investigated by means of laser-induced fluorescence (LIF) after i.v. injection into 38 tumour-bearing (MS-2 fibrosarcoma) female Balb/c mice. At 3, 24, 48 or 96 h after administration, the carotenoporphyrin fluorescence was measured in tumoral and peritumoral tissue, as well as in the abdominal, thoracic and cranial cavities. The fluorescence was induced by a nitrogen laser-pumped dye laser, emitting light at 425 nm, and analysed by a polychromator equipped with an image-intensified CCD camera. The fluorescence was evaluated at 490, 655 and 720 nm: the second and third wavelengths represent the carotenoporphyrin (CP)-related peaks, whereas the first one is close to the peak of the tissue autofluorescence. The tumour and the liver were the two tissue types showing the strongest carotenoporphyrin-related fluorescence, whereas the cerebral cortex and muscle consistently exhibited weak substance-related fluorescence. In most tissue types, the fluorescence intensities decreased over time. A few exceptions were observed, notably the liver, in which the intensity remained remarkably constant over the time period investigated.",
keywords = "Biodistribution, Carotenoporphyrin, Laser-induced fluorescence, Tumour detection",
author = "H. Nilsson and J. Johansson and K. Svanberg and S. Svanberg and G. Jori and E. Reddi and A. Segalla and Gust, {John Devens} and Moore, {Ana L} and Moore, {Thomas A}",
year = "1997",
language = "English",
volume = "76",
pages = "355--364",
journal = "British Journal of Cancer",
issn = "0007-0920",
publisher = "Nature Publishing Group",
number = "3",

}

TY - JOUR

T1 - Laser induced fluorescence studies of the biodistribution of carotenoporphyrins in mice

AU - Nilsson, H.

AU - Johansson, J.

AU - Svanberg, K.

AU - Svanberg, S.

AU - Jori, G.

AU - Reddi, E.

AU - Segalla, A.

AU - Gust, John Devens

AU - Moore, Ana L

AU - Moore, Thomas A

PY - 1997

Y1 - 1997

N2 - The biodistribution of two recently developed tumour markers, trimethylated (CP(Me)3) and trimethoxylated (CP(OMe)3) carotenoporphyrin, was investigated by means of laser-induced fluorescence (LIF) after i.v. injection into 38 tumour-bearing (MS-2 fibrosarcoma) female Balb/c mice. At 3, 24, 48 or 96 h after administration, the carotenoporphyrin fluorescence was measured in tumoral and peritumoral tissue, as well as in the abdominal, thoracic and cranial cavities. The fluorescence was induced by a nitrogen laser-pumped dye laser, emitting light at 425 nm, and analysed by a polychromator equipped with an image-intensified CCD camera. The fluorescence was evaluated at 490, 655 and 720 nm: the second and third wavelengths represent the carotenoporphyrin (CP)-related peaks, whereas the first one is close to the peak of the tissue autofluorescence. The tumour and the liver were the two tissue types showing the strongest carotenoporphyrin-related fluorescence, whereas the cerebral cortex and muscle consistently exhibited weak substance-related fluorescence. In most tissue types, the fluorescence intensities decreased over time. A few exceptions were observed, notably the liver, in which the intensity remained remarkably constant over the time period investigated.

AB - The biodistribution of two recently developed tumour markers, trimethylated (CP(Me)3) and trimethoxylated (CP(OMe)3) carotenoporphyrin, was investigated by means of laser-induced fluorescence (LIF) after i.v. injection into 38 tumour-bearing (MS-2 fibrosarcoma) female Balb/c mice. At 3, 24, 48 or 96 h after administration, the carotenoporphyrin fluorescence was measured in tumoral and peritumoral tissue, as well as in the abdominal, thoracic and cranial cavities. The fluorescence was induced by a nitrogen laser-pumped dye laser, emitting light at 425 nm, and analysed by a polychromator equipped with an image-intensified CCD camera. The fluorescence was evaluated at 490, 655 and 720 nm: the second and third wavelengths represent the carotenoporphyrin (CP)-related peaks, whereas the first one is close to the peak of the tissue autofluorescence. The tumour and the liver were the two tissue types showing the strongest carotenoporphyrin-related fluorescence, whereas the cerebral cortex and muscle consistently exhibited weak substance-related fluorescence. In most tissue types, the fluorescence intensities decreased over time. A few exceptions were observed, notably the liver, in which the intensity remained remarkably constant over the time period investigated.

KW - Biodistribution

KW - Carotenoporphyrin

KW - Laser-induced fluorescence

KW - Tumour detection

UR - http://www.scopus.com/inward/record.url?scp=12644304244&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=12644304244&partnerID=8YFLogxK

M3 - Article

C2 - 9252203

AN - SCOPUS:12644304244

VL - 76

SP - 355

EP - 364

JO - British Journal of Cancer

JF - British Journal of Cancer

SN - 0007-0920

IS - 3

ER -