Laser induced fluorescence studies of the biodistribution of carotenoporphyrins in mice

H. Nilsson, J. Johansson, K. Svanberg, S. Svanberg, G. Jori, E. Reddi, A. Segalla, John Devens Gust, Ana L Moore, Thomas A Moore

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Abstract

The biodistribution of two recently developed tumour markers, trimethylated (CP(Me)3) and trimethoxylated (CP(OMe)3) carotenoporphyrin, was investigated by means of laser-induced fluorescence (LIF) after i.v. injection into 38 tumour-bearing (MS-2 fibrosarcoma) female Balb/c mice. At 3, 24, 48 or 96 h after administration, the carotenoporphyrin fluorescence was measured in tumoral and peritumoral tissue, as well as in the abdominal, thoracic and cranial cavities. The fluorescence was induced by a nitrogen laser-pumped dye laser, emitting light at 425 nm, and analysed by a polychromator equipped with an image-intensified CCD camera. The fluorescence was evaluated at 490, 655 and 720 nm: the second and third wavelengths represent the carotenoporphyrin (CP)-related peaks, whereas the first one is close to the peak of the tissue autofluorescence. The tumour and the liver were the two tissue types showing the strongest carotenoporphyrin-related fluorescence, whereas the cerebral cortex and muscle consistently exhibited weak substance-related fluorescence. In most tissue types, the fluorescence intensities decreased over time. A few exceptions were observed, notably the liver, in which the intensity remained remarkably constant over the time period investigated.

Original languageEnglish
Pages (from-to)355-364
Number of pages10
JournalBritish Journal of Cancer
Volume76
Issue number3
Publication statusPublished - 1997

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Keywords

  • Biodistribution
  • Carotenoporphyrin
  • Laser-induced fluorescence
  • Tumour detection

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Nilsson, H., Johansson, J., Svanberg, K., Svanberg, S., Jori, G., Reddi, E., Segalla, A., Gust, J. D., Moore, A. L., & Moore, T. A. (1997). Laser induced fluorescence studies of the biodistribution of carotenoporphyrins in mice. British Journal of Cancer, 76(3), 355-364.