Light-driven production of ATP catalysed by F0F1-ATP synthase in an artificial photosynthetic membrane

Gali Steinberg-Yfrach, Jean Louis Rigaud, Edgardo N. Durantini, Ana L Moore, John Devens Gust, Thomas A Moore

Research output: Contribution to journalArticle

364 Citations (Scopus)

Abstract

Energy-transducing membranes of living organisms couple spontaneous to non-spontaneous processes through the intermediary of protonmotive force (p.m.f)-an imbalance in electrochemical potential of protons across the membrane. In most organisms, p.m.f. is generated by redox reactions that are either photochemically driven, such as those in photosynthetic reaction centres, or intrinsically spontaneous, such as those of oxidative phosphorylation in mitochondria. Transmembrane proteins (such as the cytochromes and complexes I, III and IV in the electron-transport chain in the inner mitochondrial membrane) couple the redox reactions to proton translocation, thereby conserving a fraction of the redox chemical potential as p.m.f. Many transducer proteins couple p.m.f. to the performance of biochemical work, such as biochemical synthesis and mechanical and transport processes. Recently, an artificial photosynthetic membrane was reported in which a photocyclic process was used to transport protons across a liposomal membrane, resulting in acidification of the liposome's internal volume. If significant p.m.f. is generated in this system, then incorporating an appropriate transducer into the liposomal bilayer should make it possible to drive a non-spontaneous chemical process. here we report the incorporation of F0F1-ATP synthase into liposomes containing the components of the proton- pumping photocycle. Irradiation of this artificial membrane with visible light results in the uncoupler- and inhibitor-sensitive synthesis of adenosine triphosphate (ATP) against an ATP chemical potential of ~12 kcal mol-1, with a quantum yield or more than 7%. This system mimics the process by which photosynthetic bacteria convert light energy into ATP chemical potential.

Original languageEnglish
Pages (from-to)479-482
Number of pages4
JournalNature
Volume392
Issue number6675
DOIs
Publication statusPublished - Apr 2 1998

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Artificial Membranes
Protons
Adenosine Triphosphate
Oxidation-Reduction
Light
Transducers
Liposomes
Membranes
Mechanical Phenomena
Chemical Phenomena
Photosynthetic Reaction Center Complex Proteins
Oxidative Phosphorylation
Mitochondrial Membranes
Cytochromes
Electron Transport
Mitochondria
Proteins
Bacteria

ASJC Scopus subject areas

  • General

Cite this

Light-driven production of ATP catalysed by F0F1-ATP synthase in an artificial photosynthetic membrane. / Steinberg-Yfrach, Gali; Rigaud, Jean Louis; Durantini, Edgardo N.; Moore, Ana L; Gust, John Devens; Moore, Thomas A.

In: Nature, Vol. 392, No. 6675, 02.04.1998, p. 479-482.

Research output: Contribution to journalArticle

Steinberg-Yfrach, Gali ; Rigaud, Jean Louis ; Durantini, Edgardo N. ; Moore, Ana L ; Gust, John Devens ; Moore, Thomas A. / Light-driven production of ATP catalysed by F0F1-ATP synthase in an artificial photosynthetic membrane. In: Nature. 1998 ; Vol. 392, No. 6675. pp. 479-482.
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