Light-driven quinone reduction in heliobacterial membranes

Trevor S. Kashey, Dustin D. Luu, John C. Cowgill, Patricia L. Baker, Kevin Edward Redding

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Photosynthetic reaction centers (RCs) evolved > 3 billion years ago and have diverged into Type II RCs reducing quinones and Type I RCs reducing soluble acceptors via iron–sulfur clusters. Photosystem I (PSI), the exemplar Type I RC, uses modified menaquinones as intermediate electron transfer cofactors, but it has been controversial if the Type I RC of heliobacteria (HbRC) uses its two bound menaquinones in the same way. The sequence of the quinone-binding site in PSI is not conserved in the HbRC, and the recently solved crystal structure of the HbRC does not reveal a quinone in the analogous site. We found that illumination of heliobacterial membranes resulted in reduction of menaquinone to menaquinol, suggesting that the HbRC can perform a function thought restricted to Type II RCs. Experiments on membranes and live cells are consistent with the hypothesis that the HbRC preferentially reduces soluble electron acceptors (e.g., ferredoxins) in low light, but switches to reducing lipophilic quinones in high light, when the soluble acceptor pool becomes full. Thus, the HbRC may represent a functional evolutionary intermediate between PSI and the Type II RCs.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalPhotosynthesis Research
DOIs
Publication statusAccepted/In press - Mar 12 2018

Fingerprint

Photosystem I Protein Complex
Vitamin K 2
quinones
menaquinones
photosystem I
Quinones
Membranes
Light
Electrons
Photosynthetic Reaction Center Complex Proteins
Ferredoxins
Heliobacteriaceae
photosynthetic reaction centers
Lighting
ferredoxins
crystal structure
Crystal structure
Binding Sites
Switches
Cell Membrane

Keywords

  • Heliobacteria
  • Quinone
  • Reaction centers
  • Type I reaction center
  • Type II reaction center

ASJC Scopus subject areas

  • Biochemistry
  • Plant Science
  • Cell Biology

Cite this

Light-driven quinone reduction in heliobacterial membranes. / Kashey, Trevor S.; Luu, Dustin D.; Cowgill, John C.; Baker, Patricia L.; Redding, Kevin Edward.

In: Photosynthesis Research, 12.03.2018, p. 1-9.

Research output: Contribution to journalArticle

Kashey, Trevor S. ; Luu, Dustin D. ; Cowgill, John C. ; Baker, Patricia L. ; Redding, Kevin Edward. / Light-driven quinone reduction in heliobacterial membranes. In: Photosynthesis Research. 2018 ; pp. 1-9.
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