Abstract
We demonstrate focusing and imaging through a scattering medium without access to the fluorescent object by using wavefront shaping. Our concept is based on utilizing the spatial fluorescence contrast which naturally exists in the hidden target object. By scanning the angle of incidence of the illuminating laser beam and maximizing the variation of the detected fluorescence signal from the object, as measured by a bucket detector at the front of the scattering medium, we are able to generate a tightly focused excitation spot. Thereafter, an image is obtained by scanning the focus over the object within the memory effect range. The requirements for applicability of the method and the comparison with speckle-correlation based focusing methods are discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 21778-21786 |
| Number of pages | 9 |
| Journal | Optics Express |
| Volume | 27 |
| Issue number | 15 |
| DOIs | |
| Publication status | Published - Jul 22 2019 |
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ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
Cite this
Light focusing through scattering media via linear fluorescence variance maximization, and its application for fluorescence imaging. / Daniel, Anat; Oron, Dan; Silberberg, Yaron.
In: Optics Express, Vol. 27, No. 15, 22.07.2019, p. 21778-21786.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Light focusing through scattering media via linear fluorescence variance maximization, and its application for fluorescence imaging
AU - Daniel, Anat
AU - Oron, Dan
AU - Silberberg, Yaron
PY - 2019/7/22
Y1 - 2019/7/22
N2 - We demonstrate focusing and imaging through a scattering medium without access to the fluorescent object by using wavefront shaping. Our concept is based on utilizing the spatial fluorescence contrast which naturally exists in the hidden target object. By scanning the angle of incidence of the illuminating laser beam and maximizing the variation of the detected fluorescence signal from the object, as measured by a bucket detector at the front of the scattering medium, we are able to generate a tightly focused excitation spot. Thereafter, an image is obtained by scanning the focus over the object within the memory effect range. The requirements for applicability of the method and the comparison with speckle-correlation based focusing methods are discussed.
AB - We demonstrate focusing and imaging through a scattering medium without access to the fluorescent object by using wavefront shaping. Our concept is based on utilizing the spatial fluorescence contrast which naturally exists in the hidden target object. By scanning the angle of incidence of the illuminating laser beam and maximizing the variation of the detected fluorescence signal from the object, as measured by a bucket detector at the front of the scattering medium, we are able to generate a tightly focused excitation spot. Thereafter, an image is obtained by scanning the focus over the object within the memory effect range. The requirements for applicability of the method and the comparison with speckle-correlation based focusing methods are discussed.
UR - http://www.scopus.com/inward/record.url?scp=85069874462&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85069874462&partnerID=8YFLogxK
U2 - 10.1364/OE.27.021778
DO - 10.1364/OE.27.021778
M3 - Article
VL - 27
SP - 21778
EP - 21786
JO - Optics Express
JF - Optics Express
SN - 1094-4087
IS - 15
ER -