Light-induced conformational changes in photosynthetic reaction centers: Dielectric relaxation in the vicinity of the dimer

Sasmit S. Deshmukh, Joann C. Williams, James Paul Allen, László Kálmán

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Conformational changes near the bacteriochlorophyll dimer induced by continuous illumination were identified in the wild type and 11 different mutants of reaction centers from Rhodobacter sphaeroides. The properties of the bacteriochlorophyll dimer, which has a different hydrogen bonding pattern with the surrounding protein in each mutant, were characterized by steady-state and transient optical spectroscopy. After illumination for 1 min, in the absence of the secondary quinone, the recovery of the charge-separated states was nearly 1 order of magnitude slower in one group of mutants including the wild type than in the mutants carrying the Leu to His mutation at the L131 position. The slower recovery was accompanied by a substantial decrease in the electrochromic absorption changes associated with the Qy bands of the nearby monomers during the illumination. The other set of mutants containing the Leu L131 to His substitution exhibited slightly altered electrochromic changes that decreased only half as much during the illumination as in the other family of mutants. The correlation between the recovery of the charge-separated states in the light-induced conformation and the electrochromic absorption changes suggests a dielectric relaxation of the protein that stabilizes the charge on the dimer.

Original languageEnglish
Pages (from-to)340-348
Number of pages9
JournalBiochemistry
Volume50
Issue number3
DOIs
Publication statusPublished - Jan 25 2011

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Photosynthetic Reaction Center Complex Proteins
Dielectric relaxation
Lighting
Dimers
Bacteriochlorophylls
Light
Recovery
Rhodobacter sphaeroides
Hydrogen Bonding
Conformations
Spectrum Analysis
Hydrogen bonds
Proteins
Substitution reactions
Monomers
Mutation

ASJC Scopus subject areas

  • Biochemistry

Cite this

Light-induced conformational changes in photosynthetic reaction centers : Dielectric relaxation in the vicinity of the dimer. / Deshmukh, Sasmit S.; Williams, Joann C.; Allen, James Paul; Kálmán, László.

In: Biochemistry, Vol. 50, No. 3, 25.01.2011, p. 340-348.

Research output: Contribution to journalArticle

Deshmukh, Sasmit S. ; Williams, Joann C. ; Allen, James Paul ; Kálmán, László. / Light-induced conformational changes in photosynthetic reaction centers : Dielectric relaxation in the vicinity of the dimer. In: Biochemistry. 2011 ; Vol. 50, No. 3. pp. 340-348.
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