Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography

Uwe Weierstall, Daniel James, Chong Wang, Thomas A. White, Dingjie Wang, Wei Liu, John C.H. Spence, R. Bruce Doak, Garrett Nelson, Petra Fromme, Raimund Fromme, Ingo Grotjohann, Christopher Kupitz, Nadia A. Zatsepin, Haiguang Liu, Shibom Basu, Daniel Wacker, Gye Won Han, Vsevolod Katritch, Sébastien BoutetMarc Messerschmidt, Garth J. Williams, Jason E. Koglin, M. Marvin Seibert, Markus Klinker, Cornelius Gati, Robert L. Shoeman, Anton Barty, Henry N. Chapman, Richard A. Kirian, Kenneth R. Beyerlein, Raymond C. Stevens, Dianfan Li, Syed T.A. Shah, Nicole Howe, Martin Caffrey, Vadim Cherezov

Research output: Contribution to journalArticle

320 Citations (Scopus)

Abstract

Lipidic cubic phase (LCP) crystallization has proven successful for high-resolution structure determination of challenging membrane proteins. Here we present a technique for extruding gel-like LCP with embedded membrane protein microcrystals, providing a continuously renewed source of material for serial femtosecond crystallography. Data collected from sub-10-μm-sized crystals produced with less than 0.5 mg of purified protein yield structural insights regarding cyclopamine binding to the Smoothened receptor.

Original languageEnglish
Article number3309
JournalNature communications
Volume5
DOIs
Publication statusPublished - Feb 14 2014

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Fingerprint Dive into the research topics of 'Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography'. Together they form a unique fingerprint.

  • Cite this

    Weierstall, U., James, D., Wang, C., White, T. A., Wang, D., Liu, W., Spence, J. C. H., Bruce Doak, R., Nelson, G., Fromme, P., Fromme, R., Grotjohann, I., Kupitz, C., Zatsepin, N. A., Liu, H., Basu, S., Wacker, D., Won Han, G., Katritch, V., ... Cherezov, V. (2014). Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography. Nature communications, 5, [3309]. https://doi.org/10.1038/ncomms4309