Lipidic phase membrane protein serial femtosecond crystallography

Linda C. Johansson, David Arnlund, Thomas A. White, Gergely Katona, Daniel P. Deponte, Uwe Weierstall, R. Bruce Doak, Robert L. Shoeman, Lukas Lomb, Erik Malmerberg, Jan Davidsson, Karol Nass, Mengning Liang, Jakob Andreasson, Andrew Aquila, Sasa Bajt, Miriam Barthelmess, Anton Barty, Michael J. Bogan, Christoph Bostedt & 48 others John D. Bozek, Carl Caleman, Ryan Coffee, Nicola Coppola, Tomas Ekeberg, Sascha W. Epp, Benjamin Erk, Holger Fleckenstein, Lutz Foucar, Heinz Graafsma, Lars Gumprecht, Janos Hajdu, Christina Y. Hampton, Robert Hartmann, Andreas Hartmann, Gülnter Hauser, Helmut Hirsemann, Peter Holl, Mark S. Hunter, Stephan Kassemeyer, Nils Kimmel, Richard A. Kirian, Filipe R N C Maia, Stefano Marchesini, Andrew V. Martin, Christian Reich, Daniel Rolles, Benedikt Rudek, Artem Rudenko, Ilme Schlichting, Joachim Schulz, M. Marvin Seibert, Raymond G. Sierra, Heike Soltau, Dmitri Starodub, Francesco Stellato, Stephan Stern, Lothar Strülder, Nicusor Timneanu, Joachim Ullrich, Weixiao Y. Wahlgren, Xiaoyu Wang, Georg Weidenspointner, Cornelia Wunderer, Petra Fromme, Henry N. Chapman, John C H Spence, Richard Neutze

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

X-ray free electron laser (X-FEL)-based serial femtosecond crystallography is an emerging method with potential to rapidly advance the challenging field of membrane protein structural biology. Here we recorded interpretable diffraction data from micrometer-sized lipidic sponge phase crystals of the Blastochloris viridis photosynthetic reaction center delivered into an X-FEL beam using a sponge phase micro-jet.

Original languageEnglish
Pages (from-to)263-265
Number of pages3
JournalNature Methods
Volume9
Issue number3
DOIs
Publication statusPublished - Mar 2012

Fingerprint

X ray lasers
Crystallography
Free electron lasers
Porifera
Membrane Proteins
Lasers
X-Rays
Electrons
Photosynthetic Reaction Center Complex Proteins
Laser beams
Diffraction
Crystals

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology

Cite this

Johansson, L. C., Arnlund, D., White, T. A., Katona, G., Deponte, D. P., Weierstall, U., ... Neutze, R. (2012). Lipidic phase membrane protein serial femtosecond crystallography. Nature Methods, 9(3), 263-265. https://doi.org/10.1038/nmeth.1867

Lipidic phase membrane protein serial femtosecond crystallography. / Johansson, Linda C.; Arnlund, David; White, Thomas A.; Katona, Gergely; Deponte, Daniel P.; Weierstall, Uwe; Doak, R. Bruce; Shoeman, Robert L.; Lomb, Lukas; Malmerberg, Erik; Davidsson, Jan; Nass, Karol; Liang, Mengning; Andreasson, Jakob; Aquila, Andrew; Bajt, Sasa; Barthelmess, Miriam; Barty, Anton; Bogan, Michael J.; Bostedt, Christoph; Bozek, John D.; Caleman, Carl; Coffee, Ryan; Coppola, Nicola; Ekeberg, Tomas; Epp, Sascha W.; Erk, Benjamin; Fleckenstein, Holger; Foucar, Lutz; Graafsma, Heinz; Gumprecht, Lars; Hajdu, Janos; Hampton, Christina Y.; Hartmann, Robert; Hartmann, Andreas; Hauser, Gülnter; Hirsemann, Helmut; Holl, Peter; Hunter, Mark S.; Kassemeyer, Stephan; Kimmel, Nils; Kirian, Richard A.; Maia, Filipe R N C; Marchesini, Stefano; Martin, Andrew V.; Reich, Christian; Rolles, Daniel; Rudek, Benedikt; Rudenko, Artem; Schlichting, Ilme; Schulz, Joachim; Seibert, M. Marvin; Sierra, Raymond G.; Soltau, Heike; Starodub, Dmitri; Stellato, Francesco; Stern, Stephan; Strülder, Lothar; Timneanu, Nicusor; Ullrich, Joachim; Wahlgren, Weixiao Y.; Wang, Xiaoyu; Weidenspointner, Georg; Wunderer, Cornelia; Fromme, Petra; Chapman, Henry N.; Spence, John C H; Neutze, Richard.

In: Nature Methods, Vol. 9, No. 3, 03.2012, p. 263-265.

Research output: Contribution to journalArticle

Johansson, LC, Arnlund, D, White, TA, Katona, G, Deponte, DP, Weierstall, U, Doak, RB, Shoeman, RL, Lomb, L, Malmerberg, E, Davidsson, J, Nass, K, Liang, M, Andreasson, J, Aquila, A, Bajt, S, Barthelmess, M, Barty, A, Bogan, MJ, Bostedt, C, Bozek, JD, Caleman, C, Coffee, R, Coppola, N, Ekeberg, T, Epp, SW, Erk, B, Fleckenstein, H, Foucar, L, Graafsma, H, Gumprecht, L, Hajdu, J, Hampton, CY, Hartmann, R, Hartmann, A, Hauser, G, Hirsemann, H, Holl, P, Hunter, MS, Kassemeyer, S, Kimmel, N, Kirian, RA, Maia, FRNC, Marchesini, S, Martin, AV, Reich, C, Rolles, D, Rudek, B, Rudenko, A, Schlichting, I, Schulz, J, Seibert, MM, Sierra, RG, Soltau, H, Starodub, D, Stellato, F, Stern, S, Strülder, L, Timneanu, N, Ullrich, J, Wahlgren, WY, Wang, X, Weidenspointner, G, Wunderer, C, Fromme, P, Chapman, HN, Spence, JCH & Neutze, R 2012, 'Lipidic phase membrane protein serial femtosecond crystallography', Nature Methods, vol. 9, no. 3, pp. 263-265. https://doi.org/10.1038/nmeth.1867
Johansson LC, Arnlund D, White TA, Katona G, Deponte DP, Weierstall U et al. Lipidic phase membrane protein serial femtosecond crystallography. Nature Methods. 2012 Mar;9(3):263-265. https://doi.org/10.1038/nmeth.1867
Johansson, Linda C. ; Arnlund, David ; White, Thomas A. ; Katona, Gergely ; Deponte, Daniel P. ; Weierstall, Uwe ; Doak, R. Bruce ; Shoeman, Robert L. ; Lomb, Lukas ; Malmerberg, Erik ; Davidsson, Jan ; Nass, Karol ; Liang, Mengning ; Andreasson, Jakob ; Aquila, Andrew ; Bajt, Sasa ; Barthelmess, Miriam ; Barty, Anton ; Bogan, Michael J. ; Bostedt, Christoph ; Bozek, John D. ; Caleman, Carl ; Coffee, Ryan ; Coppola, Nicola ; Ekeberg, Tomas ; Epp, Sascha W. ; Erk, Benjamin ; Fleckenstein, Holger ; Foucar, Lutz ; Graafsma, Heinz ; Gumprecht, Lars ; Hajdu, Janos ; Hampton, Christina Y. ; Hartmann, Robert ; Hartmann, Andreas ; Hauser, Gülnter ; Hirsemann, Helmut ; Holl, Peter ; Hunter, Mark S. ; Kassemeyer, Stephan ; Kimmel, Nils ; Kirian, Richard A. ; Maia, Filipe R N C ; Marchesini, Stefano ; Martin, Andrew V. ; Reich, Christian ; Rolles, Daniel ; Rudek, Benedikt ; Rudenko, Artem ; Schlichting, Ilme ; Schulz, Joachim ; Seibert, M. Marvin ; Sierra, Raymond G. ; Soltau, Heike ; Starodub, Dmitri ; Stellato, Francesco ; Stern, Stephan ; Strülder, Lothar ; Timneanu, Nicusor ; Ullrich, Joachim ; Wahlgren, Weixiao Y. ; Wang, Xiaoyu ; Weidenspointner, Georg ; Wunderer, Cornelia ; Fromme, Petra ; Chapman, Henry N. ; Spence, John C H ; Neutze, Richard. / Lipidic phase membrane protein serial femtosecond crystallography. In: Nature Methods. 2012 ; Vol. 9, No. 3. pp. 263-265.
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