Localisation and accumulation of a new carotenoporphyrin in two primary tumour models

J. T H M Van Den Akker, O. C. Speelman, H. J. Van Staveren, Ana L Moore, Thomas A Moore, John Devens Gust, W. M. Star, H. J C M Sterenborg

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We have investigated the tumour-localising properties and in vivo fluorescence kinetics of a hexamethoxylated carotenoporphyrin (CP6) in two primary tumour models: UV-B-induced early skin cancer in hairless mice and chemically induced mucosal dysplasia in the rat palate. CP6 fluorescence kinetics are investigated by measuring in vivo fluorescence spectra and images of the mouse skin and the rat palate at different time points after injection. For the tumour-localising properties, microscopic phase-contrast and fluorescence images are recorded. The in vivo fluorescence kinetics in the mouse skin show localization of CP6 in the tumours. However, fluorescence microscopy images show that CP6 localises in the dermis and structures that are not related to the malignant transformation of the mouse skin. The fluorescence kinetics in the rat palate show a significant correlation between the degree of malignancy and the CP6 fluorescence build-up time in the palate. The microscopic images show that CP6 fluorescence localises in the connective tissue and not in the dysplastic epithelium. In conclusion, CP6 does not localise preferentially in (pre-) cancerous tissue in the two primary tumour models studied here, in contrast to reports about localisation of carotenoporphyrins in transplanted tumours. However, the CP6 build-up time in rat palates correlates with the degree of malignancy and this might possibly be a useful parameter in tumour detection. (C) 2000 Elsevier Science S.A. All rights reserved.

Original languageEnglish
Pages (from-to)108-115
Number of pages8
JournalJournal of Photochemistry and Photobiology B: Biology
Volume54
Issue number2-3
DOIs
Publication statusPublished - Feb 29 2000

Fingerprint

Tumors
tumors
Fluorescence
palate
fluorescence
Palate
neoplasms
Rats
Neoplasms
Skin
rats
mice
skin (animal)
kinetics
Kinetics
Tissue
connective tissue
Hairless Mouse
dermis
Fluorescence microscopy

Keywords

  • Carotenoporphyrin
  • Fluorescence microscopy
  • Fluorescence spectroscopy
  • In vivo fluorescence kinetics
  • Tumour detection

ASJC Scopus subject areas

  • Plant Science
  • Bioengineering
  • Physical and Theoretical Chemistry

Cite this

Localisation and accumulation of a new carotenoporphyrin in two primary tumour models. / Van Den Akker, J. T H M; Speelman, O. C.; Van Staveren, H. J.; Moore, Ana L; Moore, Thomas A; Gust, John Devens; Star, W. M.; Sterenborg, H. J C M.

In: Journal of Photochemistry and Photobiology B: Biology, Vol. 54, No. 2-3, 29.02.2000, p. 108-115.

Research output: Contribution to journalArticle

Van Den Akker, J. T H M ; Speelman, O. C. ; Van Staveren, H. J. ; Moore, Ana L ; Moore, Thomas A ; Gust, John Devens ; Star, W. M. ; Sterenborg, H. J C M. / Localisation and accumulation of a new carotenoporphyrin in two primary tumour models. In: Journal of Photochemistry and Photobiology B: Biology. 2000 ; Vol. 54, No. 2-3. pp. 108-115.
@article{c391eacafce74585be92c9d3888a1ea5,
title = "Localisation and accumulation of a new carotenoporphyrin in two primary tumour models",
abstract = "We have investigated the tumour-localising properties and in vivo fluorescence kinetics of a hexamethoxylated carotenoporphyrin (CP6) in two primary tumour models: UV-B-induced early skin cancer in hairless mice and chemically induced mucosal dysplasia in the rat palate. CP6 fluorescence kinetics are investigated by measuring in vivo fluorescence spectra and images of the mouse skin and the rat palate at different time points after injection. For the tumour-localising properties, microscopic phase-contrast and fluorescence images are recorded. The in vivo fluorescence kinetics in the mouse skin show localization of CP6 in the tumours. However, fluorescence microscopy images show that CP6 localises in the dermis and structures that are not related to the malignant transformation of the mouse skin. The fluorescence kinetics in the rat palate show a significant correlation between the degree of malignancy and the CP6 fluorescence build-up time in the palate. The microscopic images show that CP6 fluorescence localises in the connective tissue and not in the dysplastic epithelium. In conclusion, CP6 does not localise preferentially in (pre-) cancerous tissue in the two primary tumour models studied here, in contrast to reports about localisation of carotenoporphyrins in transplanted tumours. However, the CP6 build-up time in rat palates correlates with the degree of malignancy and this might possibly be a useful parameter in tumour detection. (C) 2000 Elsevier Science S.A. All rights reserved.",
keywords = "Carotenoporphyrin, Fluorescence microscopy, Fluorescence spectroscopy, In vivo fluorescence kinetics, Tumour detection",
author = "{Van Den Akker}, {J. T H M} and Speelman, {O. C.} and {Van Staveren}, {H. J.} and Moore, {Ana L} and Moore, {Thomas A} and Gust, {John Devens} and Star, {W. M.} and Sterenborg, {H. J C M}",
year = "2000",
month = "2",
day = "29",
doi = "10.1016/S1011-1344(99)00157-8",
language = "English",
volume = "54",
pages = "108--115",
journal = "Journal of Photochemistry and Photobiology B: Biology",
issn = "1011-1344",
publisher = "Elsevier",
number = "2-3",

}

TY - JOUR

T1 - Localisation and accumulation of a new carotenoporphyrin in two primary tumour models

AU - Van Den Akker, J. T H M

AU - Speelman, O. C.

AU - Van Staveren, H. J.

AU - Moore, Ana L

AU - Moore, Thomas A

AU - Gust, John Devens

AU - Star, W. M.

AU - Sterenborg, H. J C M

PY - 2000/2/29

Y1 - 2000/2/29

N2 - We have investigated the tumour-localising properties and in vivo fluorescence kinetics of a hexamethoxylated carotenoporphyrin (CP6) in two primary tumour models: UV-B-induced early skin cancer in hairless mice and chemically induced mucosal dysplasia in the rat palate. CP6 fluorescence kinetics are investigated by measuring in vivo fluorescence spectra and images of the mouse skin and the rat palate at different time points after injection. For the tumour-localising properties, microscopic phase-contrast and fluorescence images are recorded. The in vivo fluorescence kinetics in the mouse skin show localization of CP6 in the tumours. However, fluorescence microscopy images show that CP6 localises in the dermis and structures that are not related to the malignant transformation of the mouse skin. The fluorescence kinetics in the rat palate show a significant correlation between the degree of malignancy and the CP6 fluorescence build-up time in the palate. The microscopic images show that CP6 fluorescence localises in the connective tissue and not in the dysplastic epithelium. In conclusion, CP6 does not localise preferentially in (pre-) cancerous tissue in the two primary tumour models studied here, in contrast to reports about localisation of carotenoporphyrins in transplanted tumours. However, the CP6 build-up time in rat palates correlates with the degree of malignancy and this might possibly be a useful parameter in tumour detection. (C) 2000 Elsevier Science S.A. All rights reserved.

AB - We have investigated the tumour-localising properties and in vivo fluorescence kinetics of a hexamethoxylated carotenoporphyrin (CP6) in two primary tumour models: UV-B-induced early skin cancer in hairless mice and chemically induced mucosal dysplasia in the rat palate. CP6 fluorescence kinetics are investigated by measuring in vivo fluorescence spectra and images of the mouse skin and the rat palate at different time points after injection. For the tumour-localising properties, microscopic phase-contrast and fluorescence images are recorded. The in vivo fluorescence kinetics in the mouse skin show localization of CP6 in the tumours. However, fluorescence microscopy images show that CP6 localises in the dermis and structures that are not related to the malignant transformation of the mouse skin. The fluorescence kinetics in the rat palate show a significant correlation between the degree of malignancy and the CP6 fluorescence build-up time in the palate. The microscopic images show that CP6 fluorescence localises in the connective tissue and not in the dysplastic epithelium. In conclusion, CP6 does not localise preferentially in (pre-) cancerous tissue in the two primary tumour models studied here, in contrast to reports about localisation of carotenoporphyrins in transplanted tumours. However, the CP6 build-up time in rat palates correlates with the degree of malignancy and this might possibly be a useful parameter in tumour detection. (C) 2000 Elsevier Science S.A. All rights reserved.

KW - Carotenoporphyrin

KW - Fluorescence microscopy

KW - Fluorescence spectroscopy

KW - In vivo fluorescence kinetics

KW - Tumour detection

UR - http://www.scopus.com/inward/record.url?scp=0034101720&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034101720&partnerID=8YFLogxK

U2 - 10.1016/S1011-1344(99)00157-8

DO - 10.1016/S1011-1344(99)00157-8

M3 - Article

C2 - 10836539

AN - SCOPUS:0034101720

VL - 54

SP - 108

EP - 115

JO - Journal of Photochemistry and Photobiology B: Biology

JF - Journal of Photochemistry and Photobiology B: Biology

SN - 1011-1344

IS - 2-3

ER -