Localization of components involved in protein transport and processing through the yeast Golgi apparatus

Alex Franzusoff, Kevin Edward Redding, Jeff Crosby, Robert S. Fuller, Randy Schekman

Research output: Contribution to journalArticle

179 Citations (Scopus)

Abstract

Saccharomyces cerevisiae sec7 mutants exhibit pleiotropic deficiencies in the transit of proteins through the Golgi apparatus, and elaborate an array of Golgi apparatus-like cisternae at a restrictive growth temperature (37°C). The SEC7 gene encodes an essential high-molecular weight protein (227 kD) that is phosphorylated in vivo. In cell lysates, Sec7 protein (Sec7p) is recovered in both sedimentable and soluble fractions. A punctate immunofluorescent pattern of Sec7p-associated structures seen in SEC cells coalesces in sec14 mutant yeast that accumulate exaggerated Golgi cisternae at 37°C. Sec7p may function as a peripheral membrane protein that cycles between a soluble, cytosolic pool and a sedimentable, membraneassociated complex for its essential role in vesicular traffic through the Golgi apparatus. The transmembrane Kex2 protease, which processes precursors of secreted peptides within the yeast secretory pathway, is also localized by indirect immunofluorescence to multiple structures in the yeast cell (Redding, K., and R. Fuller, manuscript submitted for publication). In double-immunofluorescence labeling experiments, significant colocalization of Sec7 and Kex2 proteins was found. Colocalization of the two antigens, one implicated in protein transport through the Golgi apparatus and the other in processing within a late Golgi compartment, supports the conclusion that we have visualized the yeast Golgi apparatus.

Original languageEnglish
Pages (from-to)27-37
Number of pages11
JournalJournal of Cell Biology
Volume112
Issue number1
Publication statusPublished - Jan 1991

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Golgi Apparatus
Protein Transport
Yeasts
Proteins
Manuscripts
Secretory Pathway
Essential Genes
Indirect Fluorescent Antibody Technique
Fluorescent Antibody Technique
Saccharomyces cerevisiae
Publications
Membrane Proteins
Peptide Hydrolases
Molecular Weight
Antigens
Peptides
Temperature
Growth

ASJC Scopus subject areas

  • Cell Biology

Cite this

Localization of components involved in protein transport and processing through the yeast Golgi apparatus. / Franzusoff, Alex; Redding, Kevin Edward; Crosby, Jeff; Fuller, Robert S.; Schekman, Randy.

In: Journal of Cell Biology, Vol. 112, No. 1, 01.1991, p. 27-37.

Research output: Contribution to journalArticle

Franzusoff, Alex ; Redding, Kevin Edward ; Crosby, Jeff ; Fuller, Robert S. ; Schekman, Randy. / Localization of components involved in protein transport and processing through the yeast Golgi apparatus. In: Journal of Cell Biology. 1991 ; Vol. 112, No. 1. pp. 27-37.
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