Low-Temperature Optical and Resonance Raman Spectra of a Carotenoid Cation Radical in Photosystem II

John S. Vrettos, David H. Stewart, Julio C. De Paula, Gary W Brudvig

Research output: Contribution to journalArticle

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Abstract

Low-temperature (77 K) illumination of manganese-depleted Synechocystis PCC 6803 photosystem II core complexes caused the reversible photooxidation of a carotenoid, forming a carotenoid cation radical with an absorbance maximum at 984 nm. Resonance FT-Raman spectra obtained with 1064 nm excitation gave a spectrum characteristic of carotenoid cation radicals in solution. This is the first example of a resonance Raman spectrum of a carotenoid cation radical in a protein. The carotenoid photooxidation requires prior chemical oxidation of cytochrome b559 which implicates the carotenoid in the secondary electron-transfer pathway of photosystem II that may play a role in photoprotection. The possible nature of the pathway and the structure of the carotenoid cation radical are discussed.

Original languageEnglish
Pages (from-to)6403-6406
Number of pages4
JournalJournal of Physical Chemistry B
Volume103
Issue number31
Publication statusPublished - Aug 5 1999

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carotenoids
Photosystem II Protein Complex
Carotenoids
Cations
Raman scattering
Positive ions
Raman spectra
cations
Photooxidation
photooxidation
Temperature
Proteins
cytochromes
Manganese
manganese
electron transfer
Lighting
illumination
proteins
Oxidation

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry

Cite this

Low-Temperature Optical and Resonance Raman Spectra of a Carotenoid Cation Radical in Photosystem II. / Vrettos, John S.; Stewart, David H.; De Paula, Julio C.; Brudvig, Gary W.

In: Journal of Physical Chemistry B, Vol. 103, No. 31, 05.08.1999, p. 6403-6406.

Research output: Contribution to journalArticle

Vrettos, John S. ; Stewart, David H. ; De Paula, Julio C. ; Brudvig, Gary W. / Low-Temperature Optical and Resonance Raman Spectra of a Carotenoid Cation Radical in Photosystem II. In: Journal of Physical Chemistry B. 1999 ; Vol. 103, No. 31. pp. 6403-6406.
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