Manganese and calcium requirements for reconstitution of oxygen-evolution activity in manganese-depleted photosystem II membranes

Anne Frances Miller, Gary W Brudvig

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

The Mn complex of photosystem II and O2-evolution activity are reconstituted in Mn-depleted photosystem II membranes in a light-dependent process called photoactivation. Recovery of O2-evolution activity requires both Mn2+ and Ca2+ in the photoactivation medium. The Mn2+ and Ca2+ dependences of both the effective rate constant and yield of photoactivation have been determined. A comparison of these data with the predictions of mathematical models for photoactivation leads to the conclusion that photoactivation occurs in two stages. The first stage, photoligation of Mn, requires light and depends primarily on Mn2+. The second stage, binding of Ca2+, is required for expression of O2-evolution activity. This two-stage model affords an excellent fit to the data and provides dissociation constants and binding stoichiometries for Ca2+ and Mn2+. We conclude that one Mn2+ ion is bound and photooxidized in the rate-determining step(s) of photoactivation. On the basis of these results and data already in the literature, the molecular details of the elementary steps in photoactivation are discussed and a mechanism of photoactivation is proposed.

Original languageEnglish
Pages (from-to)8181-8190
Number of pages10
JournalBiochemistry
Volume28
Issue number20
Publication statusPublished - 1989

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Photosystem II Protein Complex
Manganese
Oxygen
Calcium
Membranes
Light
Stoichiometry
Rate constants
Theoretical Models
Ions
Mathematical models
Recovery

ASJC Scopus subject areas

  • Biochemistry

Cite this

Manganese and calcium requirements for reconstitution of oxygen-evolution activity in manganese-depleted photosystem II membranes. / Miller, Anne Frances; Brudvig, Gary W.

In: Biochemistry, Vol. 28, No. 20, 1989, p. 8181-8190.

Research output: Contribution to journalArticle

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