Mechanistic insights into energy conservation by flavin-based electron bifurcation

Carolyn E. Lubner, David P. Jennings, David W. Mulder, Gerrit J. Schut, Oleg A. Zadvornyy, John P. Hoben, Monika Tokmina-Lukaszewska, Luke Berry, Diep M. Nguyen, Gina L. Lipscomb, Brian Bothner, Anne Katherine Jones, Anne Frances Miller, Paul W. King, Michael W.W. Adams, John W. Peters

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The recently realized biochemical phenomenon of energy conservation through electron bifurcation provides biology with an elegant means to maximize utilization of metabolic energy. The mechanism of coordinated coupling of exergonic and endergonic oxidation-reduction reactions by a single enzyme complex has been elucidated through optical and paramagnetic spectroscopic studies revealing unprecedented features. Pairs of electrons are bifurcated over more than 1 volt of electrochemical potential by generating a low-potential, highly energetic, unstable flavin semiquinone and directing electron flow to an iron-sulfur cluster with a highly negative potential to overcome the barrier of the endergonic half reaction. The unprecedented range of thermodynamic driving force that is generated by flavin-based electron bifurcation accounts for unique chemical reactions that are catalyzed by these enzymes.

Original languageEnglish
Pages (from-to)655-659
Number of pages5
JournalNature Chemical Biology
Volume13
Issue number6
DOIs
Publication statusPublished - Jun 1 2017

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Electrons
Biochemical Phenomena
Enzymes
Thermodynamics
Sulfur
Oxidation-Reduction
Iron
4,6-dinitro-o-cresol
flavin semiquinone

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Lubner, C. E., Jennings, D. P., Mulder, D. W., Schut, G. J., Zadvornyy, O. A., Hoben, J. P., ... Peters, J. W. (2017). Mechanistic insights into energy conservation by flavin-based electron bifurcation. Nature Chemical Biology, 13(6), 655-659. https://doi.org/10.1038/nchembio.2348

Mechanistic insights into energy conservation by flavin-based electron bifurcation. / Lubner, Carolyn E.; Jennings, David P.; Mulder, David W.; Schut, Gerrit J.; Zadvornyy, Oleg A.; Hoben, John P.; Tokmina-Lukaszewska, Monika; Berry, Luke; Nguyen, Diep M.; Lipscomb, Gina L.; Bothner, Brian; Jones, Anne Katherine; Miller, Anne Frances; King, Paul W.; Adams, Michael W.W.; Peters, John W.

In: Nature Chemical Biology, Vol. 13, No. 6, 01.06.2017, p. 655-659.

Research output: Contribution to journalArticle

Lubner, CE, Jennings, DP, Mulder, DW, Schut, GJ, Zadvornyy, OA, Hoben, JP, Tokmina-Lukaszewska, M, Berry, L, Nguyen, DM, Lipscomb, GL, Bothner, B, Jones, AK, Miller, AF, King, PW, Adams, MWW & Peters, JW 2017, 'Mechanistic insights into energy conservation by flavin-based electron bifurcation', Nature Chemical Biology, vol. 13, no. 6, pp. 655-659. https://doi.org/10.1038/nchembio.2348
Lubner CE, Jennings DP, Mulder DW, Schut GJ, Zadvornyy OA, Hoben JP et al. Mechanistic insights into energy conservation by flavin-based electron bifurcation. Nature Chemical Biology. 2017 Jun 1;13(6):655-659. https://doi.org/10.1038/nchembio.2348
Lubner, Carolyn E. ; Jennings, David P. ; Mulder, David W. ; Schut, Gerrit J. ; Zadvornyy, Oleg A. ; Hoben, John P. ; Tokmina-Lukaszewska, Monika ; Berry, Luke ; Nguyen, Diep M. ; Lipscomb, Gina L. ; Bothner, Brian ; Jones, Anne Katherine ; Miller, Anne Frances ; King, Paul W. ; Adams, Michael W.W. ; Peters, John W. / Mechanistic insights into energy conservation by flavin-based electron bifurcation. In: Nature Chemical Biology. 2017 ; Vol. 13, No. 6. pp. 655-659.
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