Melting behavior of DNA-linked polymers

Alexander Kudlay, George C Schatz

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A Flory-like theory of sharp melting of polymer-DNA hybrids is developed. The theory considers linear polymers with attached DNAs that serve as stickers, and with a two-state model governing the DNA melting equilibrium. For three or more DNA oligomers on each polymer, the solution is found to undergo phase separation at sufficiently low temperatures. The dense phase dissolves as temperature increases, which leads to a sharp increase in the fraction of non-hybridized DNA near the phase transition temperature, in agreement with known experimental absorbance profiles at 260nm. The melting temperature is predicted to be higher than that of free DNA in solution, with the amount of the increase sensitive to the importance of cooperative interactions. Modifications to the theory that take into account the cooperativity of hybridization of neighboring DNA strands are discussed.

Original languageEnglish
Title of host publication3rd Conference on Foundations of Nanoscience: Self-Assembled Architectures and Devices, FNANO 2006
Pages263-271
Number of pages9
Publication statusPublished - 2006
Event3rd Conference on Foundations of Nanoscience: Self-Assembled Architectures and Devices, FNANO 2006 - Snowbird, UT, United States
Duration: Apr 23 2006Apr 27 2006

Other

Other3rd Conference on Foundations of Nanoscience: Self-Assembled Architectures and Devices, FNANO 2006
CountryUnited States
CitySnowbird, UT
Period4/23/064/27/06

Fingerprint

Melting
DNA
Polymers
Oligomers
Phase separation
Superconducting transition temperature
Melting point
Phase transitions
Temperature

ASJC Scopus subject areas

  • Hardware and Architecture
  • Electrical and Electronic Engineering

Cite this

Kudlay, A., & Schatz, G. C. (2006). Melting behavior of DNA-linked polymers. In 3rd Conference on Foundations of Nanoscience: Self-Assembled Architectures and Devices, FNANO 2006 (pp. 263-271)

Melting behavior of DNA-linked polymers. / Kudlay, Alexander; Schatz, George C.

3rd Conference on Foundations of Nanoscience: Self-Assembled Architectures and Devices, FNANO 2006. 2006. p. 263-271.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kudlay, A & Schatz, GC 2006, Melting behavior of DNA-linked polymers. in 3rd Conference on Foundations of Nanoscience: Self-Assembled Architectures and Devices, FNANO 2006. pp. 263-271, 3rd Conference on Foundations of Nanoscience: Self-Assembled Architectures and Devices, FNANO 2006, Snowbird, UT, United States, 4/23/06.
Kudlay A, Schatz GC. Melting behavior of DNA-linked polymers. In 3rd Conference on Foundations of Nanoscience: Self-Assembled Architectures and Devices, FNANO 2006. 2006. p. 263-271
Kudlay, Alexander ; Schatz, George C. / Melting behavior of DNA-linked polymers. 3rd Conference on Foundations of Nanoscience: Self-Assembled Architectures and Devices, FNANO 2006. 2006. pp. 263-271
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