Membrane protein megahertz crystallography at the European XFEL

Chris Gisriel, Jesse Coe, Romain Letrun, Oleksandr M. Yefanov, Cesar Luna-Chavez, Natasha E. Stander, Stella Lisova, Valerio Mariani, Manuela Kuhn, Steve Aplin, Thomas D. Grant, Katerina Dörner, Tokushi Sato, Austin Echelmeier, Jorvani Cruz Villarreal, Mark S. Hunter, Max O. Wiedorn, Juraj Knoska, Victoria Mazalova, Shatabdi Roy-ChowdhuryJay How Yang, Alex Jones, Richard Bean, Johan Bielecki, Yoonhee Kim, Grant Mills, Britta Weinhausen, Jose D. Meza, Nasser Al-Qudami, Saša Bajt, Gerrit Brehm, Sabine Botha, Djelloul Boukhelef, Sandor Brockhauser, Barry D. Bruce, Matthew A. Coleman, Cyril Danilevski, Erin Discianno, Zachary Dobson, Hans Fangohr, Jose M. Martin-Garcia, Yaroslav Gevorkov, Steffen Hauf, Ahmad Hosseinizadeh, Friederike Januschek, Gihan K. Ketawala, Christopher Kupitz, Luis Maia, Maurizio Manetti, Marc Messerschmidt, Thomas Michelat, Jyotirmoy Mondal, Abbas Ourmazd, Gianpietro Previtali, Iosifina Sarrou, Silvan Schön, Peter Schwander, Megan L. Shelby, Alessandro Silenzi, Jolanta Sztuk-Dambietz, Janusz Szuba, Monica Turcato, Thomas A. White, Krzysztof Wrona, Chen Xu, Mohamed H. Abdellatif, James D. Zook, John C.H. Spence, Henry N. Chapman, Anton Barty, Richard A. Kirian, Matthias Frank, Alexandra Ros, Marius Schmidt, Raimund Fromme, Adrian P. Mancuso, Petra Fromme, Nadia A. Zatsepin

Research output: Contribution to journalArticle

Abstract

The world’s first superconducting megahertz repetition rate hard X-ray free-electron laser (XFEL), the European XFEL, began operation in 2017, featuring a unique pulse train structure with 886 ns between pulses. With its rapid pulse rate, the European XFEL may alleviate some of the increasing demand for XFEL beamtime, particularly for membrane protein serial femtosecond crystallography (SFX), leveraging orders-of-magnitude faster data collection. Here, we report the first membrane protein megahertz SFX experiment, where we determined a 2.9 Å-resolution SFX structure of the large membrane protein complex, Photosystem I, a > 1 MDa complex containing 36 protein subunits and 381 cofactors. We address challenges to megahertz SFX for membrane protein complexes, including growth of large quantities of crystals and the large molecular and unit cell size that influence data collection and analysis. The results imply that megahertz crystallography could have an important impact on structure determination of large protein complexes with XFELs.

Original languageEnglish
Article number5021
JournalNature communications
Volume10
Issue number1
DOIs
Publication statusPublished - Dec 1 2019

Fingerprint

X ray lasers
Crystallography
Free electron lasers
free electron lasers
crystallography
Membrane Proteins
Lasers
X-Rays
Electrons
membranes
proteins
x rays
Photosystem I Protein Complex
Protein Subunits
Cell Size
pulse rate
Laser pulses
Heart Rate
pulses
Crystals

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Gisriel, C., Coe, J., Letrun, R., Yefanov, O. M., Luna-Chavez, C., Stander, N. E., ... Zatsepin, N. A. (2019). Membrane protein megahertz crystallography at the European XFEL. Nature communications, 10(1), [5021]. https://doi.org/10.1038/s41467-019-12955-3

Membrane protein megahertz crystallography at the European XFEL. / Gisriel, Chris; Coe, Jesse; Letrun, Romain; Yefanov, Oleksandr M.; Luna-Chavez, Cesar; Stander, Natasha E.; Lisova, Stella; Mariani, Valerio; Kuhn, Manuela; Aplin, Steve; Grant, Thomas D.; Dörner, Katerina; Sato, Tokushi; Echelmeier, Austin; Cruz Villarreal, Jorvani; Hunter, Mark S.; Wiedorn, Max O.; Knoska, Juraj; Mazalova, Victoria; Roy-Chowdhury, Shatabdi; Yang, Jay How; Jones, Alex; Bean, Richard; Bielecki, Johan; Kim, Yoonhee; Mills, Grant; Weinhausen, Britta; Meza, Jose D.; Al-Qudami, Nasser; Bajt, Saša; Brehm, Gerrit; Botha, Sabine; Boukhelef, Djelloul; Brockhauser, Sandor; Bruce, Barry D.; Coleman, Matthew A.; Danilevski, Cyril; Discianno, Erin; Dobson, Zachary; Fangohr, Hans; Martin-Garcia, Jose M.; Gevorkov, Yaroslav; Hauf, Steffen; Hosseinizadeh, Ahmad; Januschek, Friederike; Ketawala, Gihan K.; Kupitz, Christopher; Maia, Luis; Manetti, Maurizio; Messerschmidt, Marc; Michelat, Thomas; Mondal, Jyotirmoy; Ourmazd, Abbas; Previtali, Gianpietro; Sarrou, Iosifina; Schön, Silvan; Schwander, Peter; Shelby, Megan L.; Silenzi, Alessandro; Sztuk-Dambietz, Jolanta; Szuba, Janusz; Turcato, Monica; White, Thomas A.; Wrona, Krzysztof; Xu, Chen; Abdellatif, Mohamed H.; Zook, James D.; Spence, John C.H.; Chapman, Henry N.; Barty, Anton; Kirian, Richard A.; Frank, Matthias; Ros, Alexandra; Schmidt, Marius; Fromme, Raimund; Mancuso, Adrian P.; Fromme, Petra; Zatsepin, Nadia A.

In: Nature communications, Vol. 10, No. 1, 5021, 01.12.2019.

Research output: Contribution to journalArticle

Gisriel, C, Coe, J, Letrun, R, Yefanov, OM, Luna-Chavez, C, Stander, NE, Lisova, S, Mariani, V, Kuhn, M, Aplin, S, Grant, TD, Dörner, K, Sato, T, Echelmeier, A, Cruz Villarreal, J, Hunter, MS, Wiedorn, MO, Knoska, J, Mazalova, V, Roy-Chowdhury, S, Yang, JH, Jones, A, Bean, R, Bielecki, J, Kim, Y, Mills, G, Weinhausen, B, Meza, JD, Al-Qudami, N, Bajt, S, Brehm, G, Botha, S, Boukhelef, D, Brockhauser, S, Bruce, BD, Coleman, MA, Danilevski, C, Discianno, E, Dobson, Z, Fangohr, H, Martin-Garcia, JM, Gevorkov, Y, Hauf, S, Hosseinizadeh, A, Januschek, F, Ketawala, GK, Kupitz, C, Maia, L, Manetti, M, Messerschmidt, M, Michelat, T, Mondal, J, Ourmazd, A, Previtali, G, Sarrou, I, Schön, S, Schwander, P, Shelby, ML, Silenzi, A, Sztuk-Dambietz, J, Szuba, J, Turcato, M, White, TA, Wrona, K, Xu, C, Abdellatif, MH, Zook, JD, Spence, JCH, Chapman, HN, Barty, A, Kirian, RA, Frank, M, Ros, A, Schmidt, M, Fromme, R, Mancuso, AP, Fromme, P & Zatsepin, NA 2019, 'Membrane protein megahertz crystallography at the European XFEL', Nature communications, vol. 10, no. 1, 5021. https://doi.org/10.1038/s41467-019-12955-3
Gisriel C, Coe J, Letrun R, Yefanov OM, Luna-Chavez C, Stander NE et al. Membrane protein megahertz crystallography at the European XFEL. Nature communications. 2019 Dec 1;10(1). 5021. https://doi.org/10.1038/s41467-019-12955-3
Gisriel, Chris ; Coe, Jesse ; Letrun, Romain ; Yefanov, Oleksandr M. ; Luna-Chavez, Cesar ; Stander, Natasha E. ; Lisova, Stella ; Mariani, Valerio ; Kuhn, Manuela ; Aplin, Steve ; Grant, Thomas D. ; Dörner, Katerina ; Sato, Tokushi ; Echelmeier, Austin ; Cruz Villarreal, Jorvani ; Hunter, Mark S. ; Wiedorn, Max O. ; Knoska, Juraj ; Mazalova, Victoria ; Roy-Chowdhury, Shatabdi ; Yang, Jay How ; Jones, Alex ; Bean, Richard ; Bielecki, Johan ; Kim, Yoonhee ; Mills, Grant ; Weinhausen, Britta ; Meza, Jose D. ; Al-Qudami, Nasser ; Bajt, Saša ; Brehm, Gerrit ; Botha, Sabine ; Boukhelef, Djelloul ; Brockhauser, Sandor ; Bruce, Barry D. ; Coleman, Matthew A. ; Danilevski, Cyril ; Discianno, Erin ; Dobson, Zachary ; Fangohr, Hans ; Martin-Garcia, Jose M. ; Gevorkov, Yaroslav ; Hauf, Steffen ; Hosseinizadeh, Ahmad ; Januschek, Friederike ; Ketawala, Gihan K. ; Kupitz, Christopher ; Maia, Luis ; Manetti, Maurizio ; Messerschmidt, Marc ; Michelat, Thomas ; Mondal, Jyotirmoy ; Ourmazd, Abbas ; Previtali, Gianpietro ; Sarrou, Iosifina ; Schön, Silvan ; Schwander, Peter ; Shelby, Megan L. ; Silenzi, Alessandro ; Sztuk-Dambietz, Jolanta ; Szuba, Janusz ; Turcato, Monica ; White, Thomas A. ; Wrona, Krzysztof ; Xu, Chen ; Abdellatif, Mohamed H. ; Zook, James D. ; Spence, John C.H. ; Chapman, Henry N. ; Barty, Anton ; Kirian, Richard A. ; Frank, Matthias ; Ros, Alexandra ; Schmidt, Marius ; Fromme, Raimund ; Mancuso, Adrian P. ; Fromme, Petra ; Zatsepin, Nadia A. / Membrane protein megahertz crystallography at the European XFEL. In: Nature communications. 2019 ; Vol. 10, No. 1.
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abstract = "The world’s first superconducting megahertz repetition rate hard X-ray free-electron laser (XFEL), the European XFEL, began operation in 2017, featuring a unique pulse train structure with 886 ns between pulses. With its rapid pulse rate, the European XFEL may alleviate some of the increasing demand for XFEL beamtime, particularly for membrane protein serial femtosecond crystallography (SFX), leveraging orders-of-magnitude faster data collection. Here, we report the first membrane protein megahertz SFX experiment, where we determined a 2.9 {\AA}-resolution SFX structure of the large membrane protein complex, Photosystem I, a > 1 MDa complex containing 36 protein subunits and 381 cofactors. We address challenges to megahertz SFX for membrane protein complexes, including growth of large quantities of crystals and the large molecular and unit cell size that influence data collection and analysis. The results imply that megahertz crystallography could have an important impact on structure determination of large protein complexes with XFELs.",
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AU - Coe, Jesse

AU - Letrun, Romain

AU - Yefanov, Oleksandr M.

AU - Luna-Chavez, Cesar

AU - Stander, Natasha E.

AU - Lisova, Stella

AU - Mariani, Valerio

AU - Kuhn, Manuela

AU - Aplin, Steve

AU - Grant, Thomas D.

AU - Dörner, Katerina

AU - Sato, Tokushi

AU - Echelmeier, Austin

AU - Cruz Villarreal, Jorvani

AU - Hunter, Mark S.

AU - Wiedorn, Max O.

AU - Knoska, Juraj

AU - Mazalova, Victoria

AU - Roy-Chowdhury, Shatabdi

AU - Yang, Jay How

AU - Jones, Alex

AU - Bean, Richard

AU - Bielecki, Johan

AU - Kim, Yoonhee

AU - Mills, Grant

AU - Weinhausen, Britta

AU - Meza, Jose D.

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AU - Bajt, Saša

AU - Brehm, Gerrit

AU - Botha, Sabine

AU - Boukhelef, Djelloul

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AU - Schön, Silvan

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AU - Shelby, Megan L.

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AU - Sztuk-Dambietz, Jolanta

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AU - Xu, Chen

AU - Abdellatif, Mohamed H.

AU - Zook, James D.

AU - Spence, John C.H.

AU - Chapman, Henry N.

AU - Barty, Anton

AU - Kirian, Richard A.

AU - Frank, Matthias

AU - Ros, Alexandra

AU - Schmidt, Marius

AU - Fromme, Raimund

AU - Mancuso, Adrian P.

AU - Fromme, Petra

AU - Zatsepin, Nadia A.

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