Metabolic pathways for photobiological hydrogen production by nitrogenase- and hydrogenase-containing unicellular cyanobacteria Cyanothece

Nicholas J. Skizim, Gennady M. Ananyev, Anagha Krishnan, G Charles Dismukes

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Current biotechnological interest in nitrogen-fixing cyanobacteria stems from their robust respiration and capacity to produce hydrogen. Here we quantify both dark- and light-induced H 2 effluxes by Cyanothece sp. Miami BG 043511 and establish their respective origins. Dark, anoxic H 2 production occurs via hydrogenase utilizing reductant from glycolytic catabolism of carbohydrates (autofermentation). Photo-H 2 is shown to occur via nitrogenase and requires illumination of PSI, whereas production of O 2 by co-illumination of PSII is inhibitory to nitrogenase above a threshold pO 2. Carbohydrate also serves as the major source of reductant for the PSI pathway mediated via nonphotochemical reduction of the plastoquinone pool by NADH dehydrogenases type-1 and type-2 (NDH-1 and NDH-2). Redirection of this reductant flux exclusively through the proton-coupled NDH-1 by inhibition of NDH-2 with flavone increases the photo-H 2 production rate by 2-fold (at the expense of the dark-H 2 rate), due to production of additional ATP (via the proton gradient). Comparison of photobiological hydrogen rates, yields, and energy conversion efficiencies reveals opportunities for improvement.

Original languageEnglish
Pages (from-to)2777-2786
Number of pages10
JournalJournal of Biological Chemistry
Volume287
Issue number4
DOIs
Publication statusPublished - Jan 20 2012

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Photobiological hydrogen production
Cyanothece
Hydrogenase
Nitrogenase
Reducing Agents
Cyanobacteria
Metabolic Networks and Pathways
Hydrogen
flavone
Lighting
Protons
Carbohydrates
Plastoquinone
NADH Dehydrogenase
Respiration
Nitrogen
Adenosine Triphosphate
Energy conversion
Conversion efficiency
Light

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Metabolic pathways for photobiological hydrogen production by nitrogenase- and hydrogenase-containing unicellular cyanobacteria Cyanothece. / Skizim, Nicholas J.; Ananyev, Gennady M.; Krishnan, Anagha; Dismukes, G Charles.

In: Journal of Biological Chemistry, Vol. 287, No. 4, 20.01.2012, p. 2777-2786.

Research output: Contribution to journalArticle

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