Micro- and nano-patterned elastin-like polypeptide hydrogels for stem cell culture

A. Paul, M. Stührenberg, S. Chen, D. Rhee, W. K. Lee, Teri W Odom, S. C. Heilshorn, A. Enejder

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We show that submicron-sized patterns can be imprinted into soft, recombinant-engineered protein hydrogels (here elastin-like proteins, ELP) by transferring wavy patterns from polydimethylsiloxane (PDMS) molds. The high-precision topographical tunability of the relatively stiff PDMS is translated to a bio-responsive, soft material, enabling topographical cell response studies at elastic moduli matching those of tissues. Aligned and unaligned wavy patterns with mold periodicities of 0.24-4.54 μm were imprinted and characterized by coherent anti-Stokes Raman scattering and atomic force microscopy. The pattern was successfully transferred down to 0.37 μm periodicity (width in ELP: 250 ± 50 nm, height: 70 ± 40 nm). The limit was set by inherent protein assemblies (diameter: 124-180 nm) that formed due to lower critical solution temperature behavior of the ELP during molding. The width/height of the ELP ridges depended on the degree of hydration; from complete dehydration to full hydration, ELP ridge width ranged from 79 ± 9% to 150 ± 40% of the mold width. The surface of the ridged ELP featured densely packed protein aggregates that were larger in size than those observed in bulk/flat ELP. Adipose-derived stem cells (ADSCs) oriented along hydrated aligned patterns with periodicities ≥0.60 μm (height ≥170 ± 100 nm), while random orientation was observed for smaller distances/amplitudes, as well as flat and unaligned wavy ELP surfaces. Hence, micro-molding of ELP is a promising approach to create tissue-mimicking, hierarchical architectures composed of tunable micron-sized structures with nano-sized protein aggregates, which opens the way for orthogonal screening of cell responses to topography and cell-adhesion ligands at relevant elastic moduli.

Original languageEnglish
Pages (from-to)5665-5675
Number of pages11
JournalSoft Matter
Volume13
Issue number34
DOIs
Publication statusPublished - Jan 1 2017

Fingerprint

elastin
Hydrogels
Elastin
stem cells
polypeptides
Stem cells
Cell culture
proteins
Peptides
Proteins
Molding
Hydration
periodic variations
Elastic moduli
Tissue
hydration
ridges
modulus of elasticity
Cell adhesion
Molds

ASJC Scopus subject areas

  • Chemistry(all)
  • Condensed Matter Physics

Cite this

Paul, A., Stührenberg, M., Chen, S., Rhee, D., Lee, W. K., Odom, T. W., ... Enejder, A. (2017). Micro- and nano-patterned elastin-like polypeptide hydrogels for stem cell culture. Soft Matter, 13(34), 5665-5675. https://doi.org/10.1039/c7sm00487g

Micro- and nano-patterned elastin-like polypeptide hydrogels for stem cell culture. / Paul, A.; Stührenberg, M.; Chen, S.; Rhee, D.; Lee, W. K.; Odom, Teri W; Heilshorn, S. C.; Enejder, A.

In: Soft Matter, Vol. 13, No. 34, 01.01.2017, p. 5665-5675.

Research output: Contribution to journalArticle

Paul, A, Stührenberg, M, Chen, S, Rhee, D, Lee, WK, Odom, TW, Heilshorn, SC & Enejder, A 2017, 'Micro- and nano-patterned elastin-like polypeptide hydrogels for stem cell culture', Soft Matter, vol. 13, no. 34, pp. 5665-5675. https://doi.org/10.1039/c7sm00487g
Paul, A. ; Stührenberg, M. ; Chen, S. ; Rhee, D. ; Lee, W. K. ; Odom, Teri W ; Heilshorn, S. C. ; Enejder, A. / Micro- and nano-patterned elastin-like polypeptide hydrogels for stem cell culture. In: Soft Matter. 2017 ; Vol. 13, No. 34. pp. 5665-5675.
@article{ffd09c0adc724eb6822fcd9a6afd060f,
title = "Micro- and nano-patterned elastin-like polypeptide hydrogels for stem cell culture",
abstract = "We show that submicron-sized patterns can be imprinted into soft, recombinant-engineered protein hydrogels (here elastin-like proteins, ELP) by transferring wavy patterns from polydimethylsiloxane (PDMS) molds. The high-precision topographical tunability of the relatively stiff PDMS is translated to a bio-responsive, soft material, enabling topographical cell response studies at elastic moduli matching those of tissues. Aligned and unaligned wavy patterns with mold periodicities of 0.24-4.54 μm were imprinted and characterized by coherent anti-Stokes Raman scattering and atomic force microscopy. The pattern was successfully transferred down to 0.37 μm periodicity (width in ELP: 250 ± 50 nm, height: 70 ± 40 nm). The limit was set by inherent protein assemblies (diameter: 124-180 nm) that formed due to lower critical solution temperature behavior of the ELP during molding. The width/height of the ELP ridges depended on the degree of hydration; from complete dehydration to full hydration, ELP ridge width ranged from 79 ± 9{\%} to 150 ± 40{\%} of the mold width. The surface of the ridged ELP featured densely packed protein aggregates that were larger in size than those observed in bulk/flat ELP. Adipose-derived stem cells (ADSCs) oriented along hydrated aligned patterns with periodicities ≥0.60 μm (height ≥170 ± 100 nm), while random orientation was observed for smaller distances/amplitudes, as well as flat and unaligned wavy ELP surfaces. Hence, micro-molding of ELP is a promising approach to create tissue-mimicking, hierarchical architectures composed of tunable micron-sized structures with nano-sized protein aggregates, which opens the way for orthogonal screening of cell responses to topography and cell-adhesion ligands at relevant elastic moduli.",
author = "A. Paul and M. St{\"u}hrenberg and S. Chen and D. Rhee and Lee, {W. K.} and Odom, {Teri W} and Heilshorn, {S. C.} and A. Enejder",
year = "2017",
month = "1",
day = "1",
doi = "10.1039/c7sm00487g",
language = "English",
volume = "13",
pages = "5665--5675",
journal = "Soft Matter",
issn = "1744-683X",
publisher = "Royal Society of Chemistry",
number = "34",

}

TY - JOUR

T1 - Micro- and nano-patterned elastin-like polypeptide hydrogels for stem cell culture

AU - Paul, A.

AU - Stührenberg, M.

AU - Chen, S.

AU - Rhee, D.

AU - Lee, W. K.

AU - Odom, Teri W

AU - Heilshorn, S. C.

AU - Enejder, A.

PY - 2017/1/1

Y1 - 2017/1/1

N2 - We show that submicron-sized patterns can be imprinted into soft, recombinant-engineered protein hydrogels (here elastin-like proteins, ELP) by transferring wavy patterns from polydimethylsiloxane (PDMS) molds. The high-precision topographical tunability of the relatively stiff PDMS is translated to a bio-responsive, soft material, enabling topographical cell response studies at elastic moduli matching those of tissues. Aligned and unaligned wavy patterns with mold periodicities of 0.24-4.54 μm were imprinted and characterized by coherent anti-Stokes Raman scattering and atomic force microscopy. The pattern was successfully transferred down to 0.37 μm periodicity (width in ELP: 250 ± 50 nm, height: 70 ± 40 nm). The limit was set by inherent protein assemblies (diameter: 124-180 nm) that formed due to lower critical solution temperature behavior of the ELP during molding. The width/height of the ELP ridges depended on the degree of hydration; from complete dehydration to full hydration, ELP ridge width ranged from 79 ± 9% to 150 ± 40% of the mold width. The surface of the ridged ELP featured densely packed protein aggregates that were larger in size than those observed in bulk/flat ELP. Adipose-derived stem cells (ADSCs) oriented along hydrated aligned patterns with periodicities ≥0.60 μm (height ≥170 ± 100 nm), while random orientation was observed for smaller distances/amplitudes, as well as flat and unaligned wavy ELP surfaces. Hence, micro-molding of ELP is a promising approach to create tissue-mimicking, hierarchical architectures composed of tunable micron-sized structures with nano-sized protein aggregates, which opens the way for orthogonal screening of cell responses to topography and cell-adhesion ligands at relevant elastic moduli.

AB - We show that submicron-sized patterns can be imprinted into soft, recombinant-engineered protein hydrogels (here elastin-like proteins, ELP) by transferring wavy patterns from polydimethylsiloxane (PDMS) molds. The high-precision topographical tunability of the relatively stiff PDMS is translated to a bio-responsive, soft material, enabling topographical cell response studies at elastic moduli matching those of tissues. Aligned and unaligned wavy patterns with mold periodicities of 0.24-4.54 μm were imprinted and characterized by coherent anti-Stokes Raman scattering and atomic force microscopy. The pattern was successfully transferred down to 0.37 μm periodicity (width in ELP: 250 ± 50 nm, height: 70 ± 40 nm). The limit was set by inherent protein assemblies (diameter: 124-180 nm) that formed due to lower critical solution temperature behavior of the ELP during molding. The width/height of the ELP ridges depended on the degree of hydration; from complete dehydration to full hydration, ELP ridge width ranged from 79 ± 9% to 150 ± 40% of the mold width. The surface of the ridged ELP featured densely packed protein aggregates that were larger in size than those observed in bulk/flat ELP. Adipose-derived stem cells (ADSCs) oriented along hydrated aligned patterns with periodicities ≥0.60 μm (height ≥170 ± 100 nm), while random orientation was observed for smaller distances/amplitudes, as well as flat and unaligned wavy ELP surfaces. Hence, micro-molding of ELP is a promising approach to create tissue-mimicking, hierarchical architectures composed of tunable micron-sized structures with nano-sized protein aggregates, which opens the way for orthogonal screening of cell responses to topography and cell-adhesion ligands at relevant elastic moduli.

UR - http://www.scopus.com/inward/record.url?scp=85028757717&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85028757717&partnerID=8YFLogxK

U2 - 10.1039/c7sm00487g

DO - 10.1039/c7sm00487g

M3 - Article

C2 - 28737182

AN - SCOPUS:85028757717

VL - 13

SP - 5665

EP - 5675

JO - Soft Matter

JF - Soft Matter

SN - 1744-683X

IS - 34

ER -