Abstract
Serial femtosecond crystallography (SFX) is a new emerging method, where X-ray diffraction data are collected from a fully hydrated stream of nano or microcrystals of biomolecules in their mother liquor using high-energy, X-ray free-electron lasers. The success of SFX experiments strongly depends on the ability to grow large amounts of well-ordered nano/microcrystals of homogeneous size distribution. While methods to grow large single crystals have been extensively explored in the past, method developments to grow nano/ microcrystals in sufficient amounts for SFX experiments are still in their infancy. Here, we describe and compare three methods (batch, free interface diffusion (FID) and FID centrifugation) for growth of nano/microcrystals for time-resolved SFX experiments using the large membrane protein complex photosystem II as a model system.
| Original language | English |
|---|---|
| Article number | 20130316 |
| Journal | Philosophical Transactions of the Royal Society B: Biological Sciences |
| Volume | 369 |
| Issue number | 1647 |
| DOIs | |
| Publication status | Published - Jul 17 2014 |
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Keywords
- Crystallization
- Femtosecond crystallography
- Free-electron laser
- Nanocrystals
- Photosystem II
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Medicine(all)
Cite this
Microcrystallization techniques for serial femtosecond crystallography using photosystem II from Thermosynechococcus elongatus as a model system. / Kupitz, Christopher; Grotjohann, Ingo; Conrad, Chelsie E.; Roy-Chowdhury, Shatabdi; Fromme, Raimund; Fromme, Petra.
In: Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 369, No. 1647, 20130316, 17.07.2014.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Microcrystallization techniques for serial femtosecond crystallography using photosystem II from Thermosynechococcus elongatus as a model system
AU - Kupitz, Christopher
AU - Grotjohann, Ingo
AU - Conrad, Chelsie E.
AU - Roy-Chowdhury, Shatabdi
AU - Fromme, Raimund
AU - Fromme, Petra
PY - 2014/7/17
Y1 - 2014/7/17
N2 - Serial femtosecond crystallography (SFX) is a new emerging method, where X-ray diffraction data are collected from a fully hydrated stream of nano or microcrystals of biomolecules in their mother liquor using high-energy, X-ray free-electron lasers. The success of SFX experiments strongly depends on the ability to grow large amounts of well-ordered nano/microcrystals of homogeneous size distribution. While methods to grow large single crystals have been extensively explored in the past, method developments to grow nano/ microcrystals in sufficient amounts for SFX experiments are still in their infancy. Here, we describe and compare three methods (batch, free interface diffusion (FID) and FID centrifugation) for growth of nano/microcrystals for time-resolved SFX experiments using the large membrane protein complex photosystem II as a model system.
AB - Serial femtosecond crystallography (SFX) is a new emerging method, where X-ray diffraction data are collected from a fully hydrated stream of nano or microcrystals of biomolecules in their mother liquor using high-energy, X-ray free-electron lasers. The success of SFX experiments strongly depends on the ability to grow large amounts of well-ordered nano/microcrystals of homogeneous size distribution. While methods to grow large single crystals have been extensively explored in the past, method developments to grow nano/ microcrystals in sufficient amounts for SFX experiments are still in their infancy. Here, we describe and compare three methods (batch, free interface diffusion (FID) and FID centrifugation) for growth of nano/microcrystals for time-resolved SFX experiments using the large membrane protein complex photosystem II as a model system.
KW - Crystallization
KW - Femtosecond crystallography
KW - Free-electron laser
KW - Nanocrystals
KW - Photosystem II
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UR - http://www.scopus.com/inward/citedby.url?scp=84902664473&partnerID=8YFLogxK
U2 - 10.1098/rstb.2013.0316
DO - 10.1098/rstb.2013.0316
M3 - Article
VL - 369
JO - Philosophical Transactions of the Royal Society B: Biological Sciences
JF - Philosophical Transactions of the Royal Society B: Biological Sciences
SN - 0962-8436
IS - 1647
M1 - 20130316
ER -