Abstract
Fibroblast growth factor (FGF-2) is a multifunctional growth factor that has pleiotropic effects in different tissues and organs. In particular, FGF-2 has a special role in angiogenesis, an important process in development, wound healing, cell survival, and differentiation. Therefore, incorporating biological agents like FGF-2 within therapeutic biomaterials is a potential strategy to create angiogenic bioactivity for the repair of damaged tissue caused by trauma or complications that arise from age and/or disease. However, the use of growth factors as therapeutic agents can be costly and does not always bring about efficient tissue repair due to rapid clearance from the targeted site. An alternative would be a stable supramolecular nanostructure with the capacity to activate the FGF-2 receptor that can also assemble into a scaffold deliverable to tissue. We report here on peptide amphiphiles that incorporate a peptide known to activate the FGF-2 receptor and peptide domains that drive its self-assembly into supramolecular nanoribbons. These FGF2-PA nanoribbons displayed the ability to increase the proliferation and migration of the human umbilical vein endothelial cells (HUVECs) in vitro to the same extent as the native FGF-2 protein at certain concentrations. We confirmed that this activity was specific to the FGFR1 signaling pathway by tracking the phosphorylation of downstream signaling effectors such ERK1/2 and pH3. These results indicated the specificity of FGF2-PA nanoribbons in activating the FGF-2 signaling pathway and its potential application as a supramolecular scaffold that can be used in vivo as an alternative to the encapsulation and delivery of the native FGF-2 protein.
| Original language | English |
|---|---|
| Pages (from-to) | 2166-2175 |
| Number of pages | 10 |
| Journal | ACS Biomaterials Science and Engineering |
| Volume | 3 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - Sep 11 2017 |
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Keywords
- fibroblast growth factor-2 mimetic peptide
- nanoribbons
- peptide amphiphiles
- supramolecular biomaterials
ASJC Scopus subject areas
- Biomaterials
- Biomedical Engineering
Cite this
Mimicking the Bioactivity of Fibroblast Growth Factor-2 Using Supramolecular Nanoribbons. / Rubert Pérez, Charles M.; Álvarez, Zaida; Chen, Feng; Aytun, Taner; Stupp, Samuel I.
In: ACS Biomaterials Science and Engineering, Vol. 3, No. 9, 11.09.2017, p. 2166-2175.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Mimicking the Bioactivity of Fibroblast Growth Factor-2 Using Supramolecular Nanoribbons
AU - Rubert Pérez, Charles M.
AU - Álvarez, Zaida
AU - Chen, Feng
AU - Aytun, Taner
AU - Stupp, Samuel I
PY - 2017/9/11
Y1 - 2017/9/11
N2 - Fibroblast growth factor (FGF-2) is a multifunctional growth factor that has pleiotropic effects in different tissues and organs. In particular, FGF-2 has a special role in angiogenesis, an important process in development, wound healing, cell survival, and differentiation. Therefore, incorporating biological agents like FGF-2 within therapeutic biomaterials is a potential strategy to create angiogenic bioactivity for the repair of damaged tissue caused by trauma or complications that arise from age and/or disease. However, the use of growth factors as therapeutic agents can be costly and does not always bring about efficient tissue repair due to rapid clearance from the targeted site. An alternative would be a stable supramolecular nanostructure with the capacity to activate the FGF-2 receptor that can also assemble into a scaffold deliverable to tissue. We report here on peptide amphiphiles that incorporate a peptide known to activate the FGF-2 receptor and peptide domains that drive its self-assembly into supramolecular nanoribbons. These FGF2-PA nanoribbons displayed the ability to increase the proliferation and migration of the human umbilical vein endothelial cells (HUVECs) in vitro to the same extent as the native FGF-2 protein at certain concentrations. We confirmed that this activity was specific to the FGFR1 signaling pathway by tracking the phosphorylation of downstream signaling effectors such ERK1/2 and pH3. These results indicated the specificity of FGF2-PA nanoribbons in activating the FGF-2 signaling pathway and its potential application as a supramolecular scaffold that can be used in vivo as an alternative to the encapsulation and delivery of the native FGF-2 protein.
AB - Fibroblast growth factor (FGF-2) is a multifunctional growth factor that has pleiotropic effects in different tissues and organs. In particular, FGF-2 has a special role in angiogenesis, an important process in development, wound healing, cell survival, and differentiation. Therefore, incorporating biological agents like FGF-2 within therapeutic biomaterials is a potential strategy to create angiogenic bioactivity for the repair of damaged tissue caused by trauma or complications that arise from age and/or disease. However, the use of growth factors as therapeutic agents can be costly and does not always bring about efficient tissue repair due to rapid clearance from the targeted site. An alternative would be a stable supramolecular nanostructure with the capacity to activate the FGF-2 receptor that can also assemble into a scaffold deliverable to tissue. We report here on peptide amphiphiles that incorporate a peptide known to activate the FGF-2 receptor and peptide domains that drive its self-assembly into supramolecular nanoribbons. These FGF2-PA nanoribbons displayed the ability to increase the proliferation and migration of the human umbilical vein endothelial cells (HUVECs) in vitro to the same extent as the native FGF-2 protein at certain concentrations. We confirmed that this activity was specific to the FGFR1 signaling pathway by tracking the phosphorylation of downstream signaling effectors such ERK1/2 and pH3. These results indicated the specificity of FGF2-PA nanoribbons in activating the FGF-2 signaling pathway and its potential application as a supramolecular scaffold that can be used in vivo as an alternative to the encapsulation and delivery of the native FGF-2 protein.
KW - fibroblast growth factor-2 mimetic peptide
KW - nanoribbons
KW - peptide amphiphiles
KW - supramolecular biomaterials
UR - http://www.scopus.com/inward/record.url?scp=85029452878&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85029452878&partnerID=8YFLogxK
U2 - 10.1021/acsbiomaterials.7b00347
DO - 10.1021/acsbiomaterials.7b00347
M3 - Article
AN - SCOPUS:85029452878
VL - 3
SP - 2166
EP - 2175
JO - ACS Biomaterials Science and Engineering
JF - ACS Biomaterials Science and Engineering
SN - 2373-9878
IS - 9
ER -