Mimicking the Bioactivity of Fibroblast Growth Factor-2 Using Supramolecular Nanoribbons

Charles M. Rubert Pérez, Zaida Álvarez, Feng Chen, Taner Aytun, Samuel I Stupp

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Fibroblast growth factor (FGF-2) is a multifunctional growth factor that has pleiotropic effects in different tissues and organs. In particular, FGF-2 has a special role in angiogenesis, an important process in development, wound healing, cell survival, and differentiation. Therefore, incorporating biological agents like FGF-2 within therapeutic biomaterials is a potential strategy to create angiogenic bioactivity for the repair of damaged tissue caused by trauma or complications that arise from age and/or disease. However, the use of growth factors as therapeutic agents can be costly and does not always bring about efficient tissue repair due to rapid clearance from the targeted site. An alternative would be a stable supramolecular nanostructure with the capacity to activate the FGF-2 receptor that can also assemble into a scaffold deliverable to tissue. We report here on peptide amphiphiles that incorporate a peptide known to activate the FGF-2 receptor and peptide domains that drive its self-assembly into supramolecular nanoribbons. These FGF2-PA nanoribbons displayed the ability to increase the proliferation and migration of the human umbilical vein endothelial cells (HUVECs) in vitro to the same extent as the native FGF-2 protein at certain concentrations. We confirmed that this activity was specific to the FGFR1 signaling pathway by tracking the phosphorylation of downstream signaling effectors such ERK1/2 and pH3. These results indicated the specificity of FGF2-PA nanoribbons in activating the FGF-2 signaling pathway and its potential application as a supramolecular scaffold that can be used in vivo as an alternative to the encapsulation and delivery of the native FGF-2 protein.

Original languageEnglish
Pages (from-to)2166-2175
Number of pages10
JournalACS Biomaterials Science and Engineering
Volume3
Issue number9
DOIs
Publication statusPublished - Sep 11 2017

Fingerprint

Nanoribbons
Carbon Nanotubes
Fibroblast Growth Factor 2
Fibroblasts
Bioactivity
Tissue
Peptides
Repair
Proteins
Amphiphiles
Phosphorylation
Endothelial cells
Scaffolds (biology)
Encapsulation
Biomaterials
Scaffolds
Self assembly
Nanostructures
Cells
Intercellular Signaling Peptides and Proteins

Keywords

  • fibroblast growth factor-2 mimetic peptide
  • nanoribbons
  • peptide amphiphiles
  • supramolecular biomaterials

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

Cite this

Mimicking the Bioactivity of Fibroblast Growth Factor-2 Using Supramolecular Nanoribbons. / Rubert Pérez, Charles M.; Álvarez, Zaida; Chen, Feng; Aytun, Taner; Stupp, Samuel I.

In: ACS Biomaterials Science and Engineering, Vol. 3, No. 9, 11.09.2017, p. 2166-2175.

Research output: Contribution to journalArticle

Rubert Pérez, Charles M. ; Álvarez, Zaida ; Chen, Feng ; Aytun, Taner ; Stupp, Samuel I. / Mimicking the Bioactivity of Fibroblast Growth Factor-2 Using Supramolecular Nanoribbons. In: ACS Biomaterials Science and Engineering. 2017 ; Vol. 3, No. 9. pp. 2166-2175.
@article{c953596d66d84dae99844912c5a2b370,
title = "Mimicking the Bioactivity of Fibroblast Growth Factor-2 Using Supramolecular Nanoribbons",
abstract = "Fibroblast growth factor (FGF-2) is a multifunctional growth factor that has pleiotropic effects in different tissues and organs. In particular, FGF-2 has a special role in angiogenesis, an important process in development, wound healing, cell survival, and differentiation. Therefore, incorporating biological agents like FGF-2 within therapeutic biomaterials is a potential strategy to create angiogenic bioactivity for the repair of damaged tissue caused by trauma or complications that arise from age and/or disease. However, the use of growth factors as therapeutic agents can be costly and does not always bring about efficient tissue repair due to rapid clearance from the targeted site. An alternative would be a stable supramolecular nanostructure with the capacity to activate the FGF-2 receptor that can also assemble into a scaffold deliverable to tissue. We report here on peptide amphiphiles that incorporate a peptide known to activate the FGF-2 receptor and peptide domains that drive its self-assembly into supramolecular nanoribbons. These FGF2-PA nanoribbons displayed the ability to increase the proliferation and migration of the human umbilical vein endothelial cells (HUVECs) in vitro to the same extent as the native FGF-2 protein at certain concentrations. We confirmed that this activity was specific to the FGFR1 signaling pathway by tracking the phosphorylation of downstream signaling effectors such ERK1/2 and pH3. These results indicated the specificity of FGF2-PA nanoribbons in activating the FGF-2 signaling pathway and its potential application as a supramolecular scaffold that can be used in vivo as an alternative to the encapsulation and delivery of the native FGF-2 protein.",
keywords = "fibroblast growth factor-2 mimetic peptide, nanoribbons, peptide amphiphiles, supramolecular biomaterials",
author = "{Rubert P{\'e}rez}, {Charles M.} and Zaida {\'A}lvarez and Feng Chen and Taner Aytun and Stupp, {Samuel I}",
year = "2017",
month = "9",
day = "11",
doi = "10.1021/acsbiomaterials.7b00347",
language = "English",
volume = "3",
pages = "2166--2175",
journal = "ACS Biomaterials Science and Engineering",
issn = "2373-9878",
publisher = "American Chemical Society",
number = "9",

}

TY - JOUR

T1 - Mimicking the Bioactivity of Fibroblast Growth Factor-2 Using Supramolecular Nanoribbons

AU - Rubert Pérez, Charles M.

AU - Álvarez, Zaida

AU - Chen, Feng

AU - Aytun, Taner

AU - Stupp, Samuel I

PY - 2017/9/11

Y1 - 2017/9/11

N2 - Fibroblast growth factor (FGF-2) is a multifunctional growth factor that has pleiotropic effects in different tissues and organs. In particular, FGF-2 has a special role in angiogenesis, an important process in development, wound healing, cell survival, and differentiation. Therefore, incorporating biological agents like FGF-2 within therapeutic biomaterials is a potential strategy to create angiogenic bioactivity for the repair of damaged tissue caused by trauma or complications that arise from age and/or disease. However, the use of growth factors as therapeutic agents can be costly and does not always bring about efficient tissue repair due to rapid clearance from the targeted site. An alternative would be a stable supramolecular nanostructure with the capacity to activate the FGF-2 receptor that can also assemble into a scaffold deliverable to tissue. We report here on peptide amphiphiles that incorporate a peptide known to activate the FGF-2 receptor and peptide domains that drive its self-assembly into supramolecular nanoribbons. These FGF2-PA nanoribbons displayed the ability to increase the proliferation and migration of the human umbilical vein endothelial cells (HUVECs) in vitro to the same extent as the native FGF-2 protein at certain concentrations. We confirmed that this activity was specific to the FGFR1 signaling pathway by tracking the phosphorylation of downstream signaling effectors such ERK1/2 and pH3. These results indicated the specificity of FGF2-PA nanoribbons in activating the FGF-2 signaling pathway and its potential application as a supramolecular scaffold that can be used in vivo as an alternative to the encapsulation and delivery of the native FGF-2 protein.

AB - Fibroblast growth factor (FGF-2) is a multifunctional growth factor that has pleiotropic effects in different tissues and organs. In particular, FGF-2 has a special role in angiogenesis, an important process in development, wound healing, cell survival, and differentiation. Therefore, incorporating biological agents like FGF-2 within therapeutic biomaterials is a potential strategy to create angiogenic bioactivity for the repair of damaged tissue caused by trauma or complications that arise from age and/or disease. However, the use of growth factors as therapeutic agents can be costly and does not always bring about efficient tissue repair due to rapid clearance from the targeted site. An alternative would be a stable supramolecular nanostructure with the capacity to activate the FGF-2 receptor that can also assemble into a scaffold deliverable to tissue. We report here on peptide amphiphiles that incorporate a peptide known to activate the FGF-2 receptor and peptide domains that drive its self-assembly into supramolecular nanoribbons. These FGF2-PA nanoribbons displayed the ability to increase the proliferation and migration of the human umbilical vein endothelial cells (HUVECs) in vitro to the same extent as the native FGF-2 protein at certain concentrations. We confirmed that this activity was specific to the FGFR1 signaling pathway by tracking the phosphorylation of downstream signaling effectors such ERK1/2 and pH3. These results indicated the specificity of FGF2-PA nanoribbons in activating the FGF-2 signaling pathway and its potential application as a supramolecular scaffold that can be used in vivo as an alternative to the encapsulation and delivery of the native FGF-2 protein.

KW - fibroblast growth factor-2 mimetic peptide

KW - nanoribbons

KW - peptide amphiphiles

KW - supramolecular biomaterials

UR - http://www.scopus.com/inward/record.url?scp=85029452878&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85029452878&partnerID=8YFLogxK

U2 - 10.1021/acsbiomaterials.7b00347

DO - 10.1021/acsbiomaterials.7b00347

M3 - Article

AN - SCOPUS:85029452878

VL - 3

SP - 2166

EP - 2175

JO - ACS Biomaterials Science and Engineering

JF - ACS Biomaterials Science and Engineering

SN - 2373-9878

IS - 9

ER -