Modulation of function in a minimalist heme-binding membrane protein

Sandip Shinde, Jeanine M. Cordova, Brian W. Woodrum, Giovanna Ghirlanda

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

De novo designed heme-binding proteins have been used successfully to recapitulate features of natural hemoproteins. This approach has now been extended to membrane-soluble model proteins. Our group designed a functional hemoprotein, ME1, by engineering a bishistidine binding site into a natural membrane protein, glycophorin A (Cordova et al. in J Am Chem Soc 129:512-518, 2007). ME1 binds iron(III) protoporphyrin IX with submicromolar affinity, has a redox potential of -128 mV, and displays peroxidase activity. Here, we show the effect of aromatic residues in modulating the redox potential in the context of a membrane-soluble model system. We designed aromatic interactions with the heme through a single-point mutant, G25F, in which a phenylalanine is designed to dock against the porphyrin ring. This mutation results in roughly tenfold tighter binding to iron(III) protoporphyrin IX (Kd,app = 6.5 9 10-8 M), and lowers the redox potential of the cofactor to -172 mV. This work demonstrates that specific design features aimed at controlling the properties of bound cofactors can be introduced in a minimalist membrane hemoprotein model. The ability to modulate the redox potential of cofactors embedded in artificial membrane proteins is crucial for the design of electron transfer chains across membranes in functional photosynthetic devices.

Original languageEnglish
Pages (from-to)557-564
Number of pages8
JournalJournal of Biological Inorganic Chemistry
Volume17
Issue number4
DOIs
Publication statusPublished - Apr 2012

Fingerprint

Heme
Oxidation-Reduction
Membrane Proteins
Modulation
Membranes
Iron
Glycophorin
Artificial Membranes
Docks
Porphyrins
Phenylalanine
Application programs
Peroxidase
Binding Sites
Electrons
Equipment and Supplies
Mutation
heme-binding protein
Proteins
protoporphyrin IX

Keywords

  • De novo design
  • Heme
  • Membrane protein
  • Redox potential

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry

Cite this

Modulation of function in a minimalist heme-binding membrane protein. / Shinde, Sandip; Cordova, Jeanine M.; Woodrum, Brian W.; Ghirlanda, Giovanna.

In: Journal of Biological Inorganic Chemistry, Vol. 17, No. 4, 04.2012, p. 557-564.

Research output: Contribution to journalArticle

Shinde, Sandip ; Cordova, Jeanine M. ; Woodrum, Brian W. ; Ghirlanda, Giovanna. / Modulation of function in a minimalist heme-binding membrane protein. In: Journal of Biological Inorganic Chemistry. 2012 ; Vol. 17, No. 4. pp. 557-564.
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