Molecular robots guided by prescriptive landscapes

Kyle Lund, Anthony J. Manzo, Nadine Dabby, Nicole Michelotti, Alexander Johnson-Buck, Jeanette Nangreave, Steven Taylor, Renjun Pei, Milan N. Stojanovic, Nils G. Walter, Erik Winfree, Hao Yan

Research output: Contribution to journalArticle

531 Citations (Scopus)

Abstract

Traditional robots1 rely for their function on computing, to store internal representations of their goals and environment and to coordinate sensing and any actuation of components required in response. Moving robotics to the single-molecule level is possible in principle, but requires facing the limited ability of individual molecules to store complex information and programs. One strategy to overcome this problem is to use systems that can obtain complex behaviour from the interaction of simple robots with their environment2-4. A first step in this direction was the development of DNA walkers, which have developed from being non-autonomous6,7 to being capable of directed but brief motion on one-dimensional tracks 8-11. Here we demonstrate that previously developed random walkersg12-so-called molecular spiders that comprise a streptavidin molecule as an inert body and three deoxyribozymes as catalytic legsg show elementary robotic behaviour when interacting with a precisely defined environment. Single-molecule microscopy observations confirm that such walkers achieve directional movement by sensing and modifying tracks of substrate molecules laid out on a two-dimensional DNA origami landscape. When using appropriately designed DNA origami, the molecular spiders autonomously carry out sequences of actions such as ĝ€̃ startĝ€™, ĝ€̃ followĝ€™, ĝ€̃ turnĝ€™ and ĝ€̃ stopĝ€™. We anticipate that this strategy will result in more complex robotic behaviour at the molecular level if additional control mechanisms are incorporated. One example might be interactions between multiple molecular robots leading to collective behaviour; another might be the ability to read and transform secondary cues on the DNA origami landscape as a means of implementing Turing-universal algorithmic behaviour.

Original languageEnglish
Pages (from-to)206-209
Number of pages4
JournalNature
Volume465
Issue number7295
DOIs
Publication statusPublished - May 13 2010

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Robotics
Walkers
Spiders
Aptitude
DNA
Catalytic DNA
Streptavidin
Cues

ASJC Scopus subject areas

  • General

Cite this

Lund, K., Manzo, A. J., Dabby, N., Michelotti, N., Johnson-Buck, A., Nangreave, J., ... Yan, H. (2010). Molecular robots guided by prescriptive landscapes. Nature, 465(7295), 206-209. https://doi.org/10.1038/nature09012

Molecular robots guided by prescriptive landscapes. / Lund, Kyle; Manzo, Anthony J.; Dabby, Nadine; Michelotti, Nicole; Johnson-Buck, Alexander; Nangreave, Jeanette; Taylor, Steven; Pei, Renjun; Stojanovic, Milan N.; Walter, Nils G.; Winfree, Erik; Yan, Hao.

In: Nature, Vol. 465, No. 7295, 13.05.2010, p. 206-209.

Research output: Contribution to journalArticle

Lund, K, Manzo, AJ, Dabby, N, Michelotti, N, Johnson-Buck, A, Nangreave, J, Taylor, S, Pei, R, Stojanovic, MN, Walter, NG, Winfree, E & Yan, H 2010, 'Molecular robots guided by prescriptive landscapes', Nature, vol. 465, no. 7295, pp. 206-209. https://doi.org/10.1038/nature09012
Lund K, Manzo AJ, Dabby N, Michelotti N, Johnson-Buck A, Nangreave J et al. Molecular robots guided by prescriptive landscapes. Nature. 2010 May 13;465(7295):206-209. https://doi.org/10.1038/nature09012
Lund, Kyle ; Manzo, Anthony J. ; Dabby, Nadine ; Michelotti, Nicole ; Johnson-Buck, Alexander ; Nangreave, Jeanette ; Taylor, Steven ; Pei, Renjun ; Stojanovic, Milan N. ; Walter, Nils G. ; Winfree, Erik ; Yan, Hao. / Molecular robots guided by prescriptive landscapes. In: Nature. 2010 ; Vol. 465, No. 7295. pp. 206-209.
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