Mucin-Pseudomonas aeruginosa interactions promote biofilm formation and antibiotic resistance

Rebecca M. Landry, Dingding An, Joseph T Hupp, Pradeep K. Singh, Matthew R. Parsek

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

Pseudomonas aeruginosa is an opportunistic pathogen that causes chronic lung infections in people suffering from cystic fibrosis (CF). In CF airways, P. aeruginosa forms surface-associated communities called biofilms. Compared with free-swimming cultures, biofilms resist clearance by the host immune system and display increased resistance to antimicrobial agents. In this study we developed a technique to coat surfaces with molecules that are abundant in CF airways in order to investigate their impact on P. aeruginosa biofilm development. We found that P. aeruginosa biofilm development proceeds differently on surfaces coated with the glycoprotein mucin compared with biofilm development on glass and surfaces coated with actin or DNA. Biofilms formed on mucin-coated surfaces developed large cellular aggregates and had increased tolerance to the antibiotic tobramycin compared with biofilms grown on glass. Analysis of selected mutant backgrounds in conjunction with time-lapse microscopy revealed that surface-associated motility was blocked on the mucin surface. Furthermore, our data suggest that a specific adhesin-mucin interaction immobilizes the bacterium on the surface. Together, these experiments suggest that mucin, which may serve as an attachment surface in CF airways, impacts P. aeruginosa biofilm development and function.

Original languageEnglish
Pages (from-to)142-151
Number of pages10
JournalMolecular Microbiology
Volume59
Issue number1
DOIs
Publication statusPublished - Jan 2006

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Mucins
Biofilms
Microbial Drug Resistance
Pseudomonas aeruginosa
Cystic Fibrosis
Glass
Tobramycin
Anti-Infective Agents
Actins
Microscopy
Immune System
Glycoproteins
Anti-Bacterial Agents
Bacteria
Lung
DNA
Infection

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

Mucin-Pseudomonas aeruginosa interactions promote biofilm formation and antibiotic resistance. / Landry, Rebecca M.; An, Dingding; Hupp, Joseph T; Singh, Pradeep K.; Parsek, Matthew R.

In: Molecular Microbiology, Vol. 59, No. 1, 01.2006, p. 142-151.

Research output: Contribution to journalArticle

Landry, Rebecca M. ; An, Dingding ; Hupp, Joseph T ; Singh, Pradeep K. ; Parsek, Matthew R. / Mucin-Pseudomonas aeruginosa interactions promote biofilm formation and antibiotic resistance. In: Molecular Microbiology. 2006 ; Vol. 59, No. 1. pp. 142-151.
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