Abstract
Depth resolved multiphoton microscopy is performed by collecting the fluorescent emission of two-exciton states in colloidal quantum dots. The biexciton is formed via two sequential resonant absorption events. Due to the large absorption cross-section and the long lifetime of the intermediate (singly excited) state, unprecedented low excitation energy and peak powers (down to 105W/cm2) are required to generate this nonlinear response.Depending on the quantum dot parameters, the effective two-photon cross section can be as large as 1010 GM,orders of magnitude higher than for nonresonant excitation. The biexciton emission can be differentiated from that of the singly excited state by utilizing its different transient dynamics. Alternate methods for discrimination are also discussed. This system is ideal for performing three-dimensional microscopy using low excitation power. Moreover, it enables to perform multiphoton imaging even with near-infrared emitting quantum dots, which are highly compatible with imaging deep into a scattering tissue. The depth resolution of our microscope is shown to be equivalent to a standard two-photon microscope. The system also shows slow saturation due to the contribution of higher (triply and above) excited states to the emitted signal.
| Original language | English |
|---|---|
| Title of host publication | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
| Volume | 7183 |
| DOIs | |
| Publication status | Published - 2009 |
| Event | Multiphoton Microscopy in the Biomedical Sciences IX - San Jose, CA, United States Duration: Jan 25 2009 → Jan 27 2009 |
Other
| Other | Multiphoton Microscopy in the Biomedical Sciences IX |
|---|---|
| Country | United States |
| City | San Jose, CA |
| Period | 1/25/09 → 1/27/09 |
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Keywords
- Biexciton
- Multiphoton microscopy
- Optical sectioning
- Semiconductor quantum dots
ASJC Scopus subject areas
- Applied Mathematics
- Computer Science Applications
- Electrical and Electronic Engineering
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
Cite this
Multiphoton Microscopy by Multiexcitonic Ladder Climbing in Colloidal Quantum Dots. / Rubin, Nir; Haim, Ben; Oron, Dan.
Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7183 2009. 71831K.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Multiphoton Microscopy by Multiexcitonic Ladder Climbing in Colloidal Quantum Dots
AU - Rubin, Nir
AU - Haim, Ben
AU - Oron, Dan
PY - 2009
Y1 - 2009
N2 - Depth resolved multiphoton microscopy is performed by collecting the fluorescent emission of two-exciton states in colloidal quantum dots. The biexciton is formed via two sequential resonant absorption events. Due to the large absorption cross-section and the long lifetime of the intermediate (singly excited) state, unprecedented low excitation energy and peak powers (down to 105W/cm2) are required to generate this nonlinear response.Depending on the quantum dot parameters, the effective two-photon cross section can be as large as 1010 GM,orders of magnitude higher than for nonresonant excitation. The biexciton emission can be differentiated from that of the singly excited state by utilizing its different transient dynamics. Alternate methods for discrimination are also discussed. This system is ideal for performing three-dimensional microscopy using low excitation power. Moreover, it enables to perform multiphoton imaging even with near-infrared emitting quantum dots, which are highly compatible with imaging deep into a scattering tissue. The depth resolution of our microscope is shown to be equivalent to a standard two-photon microscope. The system also shows slow saturation due to the contribution of higher (triply and above) excited states to the emitted signal.
AB - Depth resolved multiphoton microscopy is performed by collecting the fluorescent emission of two-exciton states in colloidal quantum dots. The biexciton is formed via two sequential resonant absorption events. Due to the large absorption cross-section and the long lifetime of the intermediate (singly excited) state, unprecedented low excitation energy and peak powers (down to 105W/cm2) are required to generate this nonlinear response.Depending on the quantum dot parameters, the effective two-photon cross section can be as large as 1010 GM,orders of magnitude higher than for nonresonant excitation. The biexciton emission can be differentiated from that of the singly excited state by utilizing its different transient dynamics. Alternate methods for discrimination are also discussed. This system is ideal for performing three-dimensional microscopy using low excitation power. Moreover, it enables to perform multiphoton imaging even with near-infrared emitting quantum dots, which are highly compatible with imaging deep into a scattering tissue. The depth resolution of our microscope is shown to be equivalent to a standard two-photon microscope. The system also shows slow saturation due to the contribution of higher (triply and above) excited states to the emitted signal.
KW - Biexciton
KW - Multiphoton microscopy
KW - Optical sectioning
KW - Semiconductor quantum dots
UR - http://www.scopus.com/inward/record.url?scp=65949114845&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=65949114845&partnerID=8YFLogxK
U2 - 10.1117/12.807607
DO - 10.1117/12.807607
M3 - Conference contribution
VL - 7183
BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
ER -