Mutation induced modulation of hydrogen bonding to P700 studied using FTIR difference spectroscopy

Ruili Wang, Velautham Sivakumar, Yajing Li, Kevin Edward Redding, Gary Hastings

Research output: Contribution to journalArticle

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Abstract

Site-directed mutagenesis in combination with Fourier transform infrared difference spectroscopy has been used to study how hydrogen bonding modulates the electronic and physical organization of P700, the primary electron donor in photosystem I. Wild-type PS I particles from Chlamydomonas reinhardtii and a mutant in which ThrA739 is changed to alanine [TA(A739) mutant] were studied. ThrA739 is thought to provide a hydrogen bond to the chlorophyll-a′ molecule of P700 (the two chlorophylls of P700 (P700+) will be called PA and PB (PA+ and P B+)). The mutation considerably alters the (P700 +-P700) FTIR difference spectra. However, we were able to describe all of the mutation induced changes in the difference spectra in terms of difference band assignments that were proposed recently (Hastings, G., Ramesh, V. M., Wang, R., Sivakumar, V. and Webber, A. (2001) Biochemistry 40, 12943-12949). Upon comparison of mutant and wild type (P700+-P700) FTIR difference spectra, it is shown that (1) the 133 ester carbonyl modes of PA and PB are unaltered upon mutation of ThrA739 to alanine. (2) The 133 ester carbonyl modes of PA +/PB+ upshift/downshift upon mutation. These oppositely directed shifts indicate that the mutation modifies the charge distribution over the pigments in the P700+ state, with charge on PB being relocated onto PA. We also show that the 13 1 keto carbonyl mode of PB/PB+ is unaltered/downshifted upon mutation, as is expected for the above-described mutation induced charge redistribution in P700+. Although the 13 3 ester carbonyl modes of the chlorophylls of P700 in the ground state are unaltered upon mutation, the 131 keto carbonyl mode of PA upshifts upon mutation, as does the 131 keto carbonyl mode of PA+. For P700 in the ground state, bands that we associate with HisA676/HisB656 upshift/downshift upon mutation. For the P700+ state, bands that we associate with HisA676/HisB656 also upshift/downshift upon mutation. These observations are also consistent with the notion that the mutation leads to the charge on PB+ being relocated onto PA+. In addition, we suggest that a hydrogen bond to the 131 keto carbonyl of PA is still present in the TA(A739) mutant, probably mediated through an introduced water molecule.

Original languageEnglish
Pages (from-to)9889-9897
Number of pages9
JournalBiochemistry
Volume42
Issue number33
DOIs
Publication statusPublished - Aug 26 2003

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Fourier Transform Infrared Spectroscopy
Hydrogen Bonding
Spectrum Analysis
Hydrogen bonds
Esters
Modulation
Spectroscopy
Chlorophyll
Alanine
Ground state
Mutation
Photosystem I Protein Complex
Mutagenesis
Biochemistry
Molecules
Charge distribution
Pigments
Fourier transforms
Infrared radiation
Electrons

ASJC Scopus subject areas

  • Biochemistry

Cite this

Mutation induced modulation of hydrogen bonding to P700 studied using FTIR difference spectroscopy. / Wang, Ruili; Sivakumar, Velautham; Li, Yajing; Redding, Kevin Edward; Hastings, Gary.

In: Biochemistry, Vol. 42, No. 33, 26.08.2003, p. 9889-9897.

Research output: Contribution to journalArticle

Wang, Ruili ; Sivakumar, Velautham ; Li, Yajing ; Redding, Kevin Edward ; Hastings, Gary. / Mutation induced modulation of hydrogen bonding to P700 studied using FTIR difference spectroscopy. In: Biochemistry. 2003 ; Vol. 42, No. 33. pp. 9889-9897.
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