N-[1-13C]acetylimidazole as a reagent for tyrosyl modification in protein NMR studies: Acetylation of cytochrome c

Ronald A. Nieman; John Devens Gust; John R. Cronin

doi:10.1016/0003-2697(82)90356-6

N-[1-¹³C]acetylimidazole as a reagent for tyrosyl modification in protein NMR studies

Acetylation of cytochrome c

Ronald A. Nieman, John Devens Gust, John R. Cronin

Arizona State University

Research output: Contribution to journal › Article

2 Citations (Scopus)

Abstract

The reaction of N-[1-¹³C] acetylimidazole with cytochrome c and guanidinated cytochrome c was evaluated as a means of introducing NMR-detectable groups as conformation-dependent probes. Resonances from both N-[1-¹³C]acetyl lysyl and O-[1-¹³C]acetyl tyrosyl groups were observed when ferricytochrome c was acetylated. However, only O-[1-¹³C]acetyl tyrosyl resonances were seen with acetylated guanidinated ferricytochrome c. Chemical shifts of the four O-[1-¹³C]acetyl tyrosyl groups were conformation dependent and ranged from 172 to 176 ppm. A convenient method for the preparation of N-[1-¹³C]acetylimidazole is described.

Original language	English
Pages (from-to)	347-350
Number of pages	4
Journal	Analytical Biochemistry
Volume	120
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(82)90356-6
Publication status	Published - Mar 1 1982

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Acetylation

Cytochromes c

Nuclear magnetic resonance

Conformations

Proteins

Chemical shift

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

Cite this

Nieman, R. A., Gust, J. D., & Cronin, J. R. (1982). N-[1-¹³C]acetylimidazole as a reagent for tyrosyl modification in protein NMR studies: Acetylation of cytochrome c. Analytical Biochemistry, 120(2), 347-350. https://doi.org/10.1016/0003-2697(82)90356-6

N-[1-¹³C]acetylimidazole as a reagent for tyrosyl modification in protein NMR studies : Acetylation of cytochrome c. / Nieman, Ronald A.; Gust, John Devens; Cronin, John R.

In: Analytical Biochemistry, Vol. 120, No. 2, 01.03.1982, p. 347-350.

Research output: Contribution to journal › Article

Nieman, RA, Gust, JD & Cronin, JR 1982, 'N-[1-¹³C]acetylimidazole as a reagent for tyrosyl modification in protein NMR studies: Acetylation of cytochrome c', Analytical Biochemistry, vol. 120, no. 2, pp. 347-350. https://doi.org/10.1016/0003-2697(82)90356-6

Nieman RA, Gust JD, Cronin JR. N-[1-¹³C]acetylimidazole as a reagent for tyrosyl modification in protein NMR studies: Acetylation of cytochrome c. Analytical Biochemistry. 1982 Mar 1;120(2):347-350. https://doi.org/10.1016/0003-2697(82)90356-6

Nieman, Ronald A. ; Gust, John Devens ; Cronin, John R. / N-[1-¹³C]acetylimidazole as a reagent for tyrosyl modification in protein NMR studies : Acetylation of cytochrome c. In: Analytical Biochemistry. 1982 ; Vol. 120, No. 2. pp. 347-350.

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