Abstract
The reaction of N-[1-13C] acetylimidazole with cytochrome c and guanidinated cytochrome c was evaluated as a means of introducing NMR-detectable groups as conformation-dependent probes. Resonances from both N-[1-13C]acetyl lysyl and O-[1-13C]acetyl tyrosyl groups were observed when ferricytochrome c was acetylated. However, only O-[1-13C]acetyl tyrosyl resonances were seen with acetylated guanidinated ferricytochrome c. Chemical shifts of the four O-[1-13C]acetyl tyrosyl groups were conformation dependent and ranged from 172 to 176 ppm. A convenient method for the preparation of N-[1-13C]acetylimidazole is described.
Original language | English |
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Pages (from-to) | 347-350 |
Number of pages | 4 |
Journal | Analytical Biochemistry |
Volume | 120 |
Issue number | 2 |
DOIs | |
Publication status | Published - Mar 1 1982 |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
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N-[1-13C]acetylimidazole as a reagent for tyrosyl modification in protein NMR studies : Acetylation of cytochrome c. / Nieman, Ronald A.; Gust, John Devens; Cronin, John R.
In: Analytical Biochemistry, Vol. 120, No. 2, 01.03.1982, p. 347-350.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - N-[1-13C]acetylimidazole as a reagent for tyrosyl modification in protein NMR studies
T2 - Acetylation of cytochrome c
AU - Nieman, Ronald A.
AU - Gust, John Devens
AU - Cronin, John R.
PY - 1982/3/1
Y1 - 1982/3/1
N2 - The reaction of N-[1-13C] acetylimidazole with cytochrome c and guanidinated cytochrome c was evaluated as a means of introducing NMR-detectable groups as conformation-dependent probes. Resonances from both N-[1-13C]acetyl lysyl and O-[1-13C]acetyl tyrosyl groups were observed when ferricytochrome c was acetylated. However, only O-[1-13C]acetyl tyrosyl resonances were seen with acetylated guanidinated ferricytochrome c. Chemical shifts of the four O-[1-13C]acetyl tyrosyl groups were conformation dependent and ranged from 172 to 176 ppm. A convenient method for the preparation of N-[1-13C]acetylimidazole is described.
AB - The reaction of N-[1-13C] acetylimidazole with cytochrome c and guanidinated cytochrome c was evaluated as a means of introducing NMR-detectable groups as conformation-dependent probes. Resonances from both N-[1-13C]acetyl lysyl and O-[1-13C]acetyl tyrosyl groups were observed when ferricytochrome c was acetylated. However, only O-[1-13C]acetyl tyrosyl resonances were seen with acetylated guanidinated ferricytochrome c. Chemical shifts of the four O-[1-13C]acetyl tyrosyl groups were conformation dependent and ranged from 172 to 176 ppm. A convenient method for the preparation of N-[1-13C]acetylimidazole is described.
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UR - http://www.scopus.com/inward/citedby.url?scp=0020110690&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(82)90356-6
DO - 10.1016/0003-2697(82)90356-6
M3 - Article
C2 - 6283939
AN - SCOPUS:0020110690
VL - 120
SP - 347
EP - 350
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 2
ER -