Abstract
In this paper, we report critical design rules for improving the sensitivity of plasmonic biosensors. We use a scalable, parallel method to fabricate Au plasmonic crystal substrates patterned with an array of pits for real-time sensing. Capture ligands printed selectively in these pits resulted in a strong response to the binding of macromolecules. Angle-resolved sensorgrams showed quantitative differences between wavelength shifts produced by protein analyte bound within regions supporting localized and long-range electromagnetic fields. Contributions from the localized fields dominated the sensing response, especially at high angles. The angle-dependent far-field and near-field optical properties of selectively functionalized plasmonic crystals suggest that optimal performance is achieved at excitation parameters that produce an intense resonance with narrow spectral bandwidth and highly localized electromagnetic fields.
| Original language | English |
|---|---|
| Pages (from-to) | 5286-5292 |
| Number of pages | 7 |
| Journal | Journal of Physical Chemistry C |
| Volume | 117 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - Mar 14 2013 |
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ASJC Scopus subject areas
- Physical and Theoretical Chemistry
- Electronic, Optical and Magnetic Materials
- Surfaces, Coatings and Films
- Energy(all)
Cite this
Nanopatterned substrates increase surface sensitivity for real-time biosensing. / Lin, Julia Y.; Stuparu, Andreea D.; Huntington, Mark D.; Mrksich, Milan; Odom, Teri W.
In: Journal of Physical Chemistry C, Vol. 117, No. 10, 14.03.2013, p. 5286-5292.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Nanopatterned substrates increase surface sensitivity for real-time biosensing
AU - Lin, Julia Y.
AU - Stuparu, Andreea D.
AU - Huntington, Mark D.
AU - Mrksich, Milan
AU - Odom, Teri W
PY - 2013/3/14
Y1 - 2013/3/14
N2 - In this paper, we report critical design rules for improving the sensitivity of plasmonic biosensors. We use a scalable, parallel method to fabricate Au plasmonic crystal substrates patterned with an array of pits for real-time sensing. Capture ligands printed selectively in these pits resulted in a strong response to the binding of macromolecules. Angle-resolved sensorgrams showed quantitative differences between wavelength shifts produced by protein analyte bound within regions supporting localized and long-range electromagnetic fields. Contributions from the localized fields dominated the sensing response, especially at high angles. The angle-dependent far-field and near-field optical properties of selectively functionalized plasmonic crystals suggest that optimal performance is achieved at excitation parameters that produce an intense resonance with narrow spectral bandwidth and highly localized electromagnetic fields.
AB - In this paper, we report critical design rules for improving the sensitivity of plasmonic biosensors. We use a scalable, parallel method to fabricate Au plasmonic crystal substrates patterned with an array of pits for real-time sensing. Capture ligands printed selectively in these pits resulted in a strong response to the binding of macromolecules. Angle-resolved sensorgrams showed quantitative differences between wavelength shifts produced by protein analyte bound within regions supporting localized and long-range electromagnetic fields. Contributions from the localized fields dominated the sensing response, especially at high angles. The angle-dependent far-field and near-field optical properties of selectively functionalized plasmonic crystals suggest that optimal performance is achieved at excitation parameters that produce an intense resonance with narrow spectral bandwidth and highly localized electromagnetic fields.
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U2 - 10.1021/jp401598a
DO - 10.1021/jp401598a
M3 - Article
VL - 117
SP - 5286
EP - 5292
JO - Journal of Physical Chemistry C
JF - Journal of Physical Chemistry C
SN - 1932-7447
IS - 10
ER -