Nanopatterned substrates increase surface sensitivity for real-time biosensing

Julia Y. Lin, Andreea D. Stuparu, Mark D. Huntington, Milan Mrksich, Teri W Odom

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In this paper, we report critical design rules for improving the sensitivity of plasmonic biosensors. We use a scalable, parallel method to fabricate Au plasmonic crystal substrates patterned with an array of pits for real-time sensing. Capture ligands printed selectively in these pits resulted in a strong response to the binding of macromolecules. Angle-resolved sensorgrams showed quantitative differences between wavelength shifts produced by protein analyte bound within regions supporting localized and long-range electromagnetic fields. Contributions from the localized fields dominated the sensing response, especially at high angles. The angle-dependent far-field and near-field optical properties of selectively functionalized plasmonic crystals suggest that optimal performance is achieved at excitation parameters that produce an intense resonance with narrow spectral bandwidth and highly localized electromagnetic fields.

Original languageEnglish
Pages (from-to)5286-5292
Number of pages7
JournalJournal of Physical Chemistry C
Volume117
Issue number10
DOIs
Publication statusPublished - Mar 14 2013

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Electromagnetic fields
Crystals
electromagnetic fields
Substrates
Macromolecules
Biosensors
Optical properties
Ligands
Proteins
Bandwidth
bioinstrumentation
macromolecules
Wavelength
crystals
far fields
near fields
proteins
bandwidth
optical properties
ligands

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Electronic, Optical and Magnetic Materials
  • Surfaces, Coatings and Films
  • Energy(all)

Cite this

Nanopatterned substrates increase surface sensitivity for real-time biosensing. / Lin, Julia Y.; Stuparu, Andreea D.; Huntington, Mark D.; Mrksich, Milan; Odom, Teri W.

In: Journal of Physical Chemistry C, Vol. 117, No. 10, 14.03.2013, p. 5286-5292.

Research output: Contribution to journalArticle

Lin, Julia Y. ; Stuparu, Andreea D. ; Huntington, Mark D. ; Mrksich, Milan ; Odom, Teri W. / Nanopatterned substrates increase surface sensitivity for real-time biosensing. In: Journal of Physical Chemistry C. 2013 ; Vol. 117, No. 10. pp. 5286-5292.
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