Near-field second harmonic imaging of granular membrane structures in natural killer cells

Richard D. Schaller; Claude Roth; David H. Raulet; Richard J. Saykally

Near-field second harmonic imaging of granular membrane structures in natural killer cells

Richard D. Schaller, Claude Roth, David H. Raulet, Richard J. Saykally

Northwestern University, Argonne National Laboratory

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

Second Harmonic Generation (SHG) has been used in combination with a near-field scanning optical microscope (NSOM) to image biological samples for the first time. Near-field SHG images of azure B stained mouse natural killer (NK) cells were recorded at four different wavelengths using a tunable near-infrared femtosecond laser. The surface specificity of SHG allows membrane structures to be selectively imaged with subdiffraction spatial resolution under ambient conditions. Resonance enhancement of SHG is shown to provide a chemically specific contrast mechanism in NSOM imaging, revealing submicron features associated with the cytolytic granular membrane.

Original language	English
Pages (from-to)	5217-5220
Number of pages	4
Journal	Journal of Physical Chemistry B
Volume	104
Issue number	22
Publication status	Published - Jun 8 2000

ASJC Scopus subject areas

Physical and Theoretical Chemistry
Surfaces, Coatings and Films
Materials Chemistry

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