Abstract
Second Harmonic Generation (SHG) has been used in combination with a near-field scanning optical microscope (NSOM) to image biological samples for the first time. Near-field SHG images of azure B stained mouse natural killer (NK) cells were recorded at four different wavelengths using a tunable near-infrared femtosecond laser. The surface specificity of SHG allows membrane structures to be selectively imaged with subdiffraction spatial resolution under ambient conditions. Resonance enhancement of SHG is shown to provide a chemically specific contrast mechanism in NSOM imaging, revealing submicron features associated with the cytolytic granular membrane.
| Original language | English |
|---|---|
| Pages (from-to) | 5217-5220 |
| Number of pages | 4 |
| Journal | Journal of Physical Chemistry B |
| Volume | 104 |
| Issue number | 22 |
| Publication status | Published - Jun 8 2000 |
ASJC Scopus subject areas
- Physical and Theoretical Chemistry
- Surfaces, Coatings and Films
- Materials Chemistry
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Schaller, R. D., Roth, C., Raulet, D. H., & Saykally, R. J. (2000). Near-field second harmonic imaging of granular membrane structures in natural killer cells. Journal of Physical Chemistry B, 104(22), 5217-5220.