Abstract
Second Harmonic Generation (SHG) has been used in combination with a near-field scanning optical microscope (NSOM) to image biological samples for the first time. Near-field SHG images of azure B stained mouse natural killer (NK) cells were recorded at four different wavelengths using a tunable near-infrared femtosecond laser. The surface specificity of SHG allows membrane structures to be selectively imaged with subdiffraction spatial resolution under ambient conditions. Resonance enhancement of SHG is shown to provide a chemically specific contrast mechanism in NSOM imaging, revealing submicron features associated with the cytolytic granular membrane.
| Original language | English |
|---|---|
| Pages (from-to) | 5217-5220 |
| Number of pages | 4 |
| Journal | Journal of Physical Chemistry B |
| Volume | 104 |
| Issue number | 22 |
| Publication status | Published - Jun 8 2000 |
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ASJC Scopus subject areas
- Physical and Theoretical Chemistry
- Engineering(all)
Cite this
Near-field second harmonic imaging of granular membrane structures in natural killer cells. / Schaller, Richard D; Roth, Claude; Raulet, David H.; Saykally, Richard J.
In: Journal of Physical Chemistry B, Vol. 104, No. 22, 08.06.2000, p. 5217-5220.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Near-field second harmonic imaging of granular membrane structures in natural killer cells
AU - Schaller, Richard D
AU - Roth, Claude
AU - Raulet, David H.
AU - Saykally, Richard J.
PY - 2000/6/8
Y1 - 2000/6/8
N2 - Second Harmonic Generation (SHG) has been used in combination with a near-field scanning optical microscope (NSOM) to image biological samples for the first time. Near-field SHG images of azure B stained mouse natural killer (NK) cells were recorded at four different wavelengths using a tunable near-infrared femtosecond laser. The surface specificity of SHG allows membrane structures to be selectively imaged with subdiffraction spatial resolution under ambient conditions. Resonance enhancement of SHG is shown to provide a chemically specific contrast mechanism in NSOM imaging, revealing submicron features associated with the cytolytic granular membrane.
AB - Second Harmonic Generation (SHG) has been used in combination with a near-field scanning optical microscope (NSOM) to image biological samples for the first time. Near-field SHG images of azure B stained mouse natural killer (NK) cells were recorded at four different wavelengths using a tunable near-infrared femtosecond laser. The surface specificity of SHG allows membrane structures to be selectively imaged with subdiffraction spatial resolution under ambient conditions. Resonance enhancement of SHG is shown to provide a chemically specific contrast mechanism in NSOM imaging, revealing submicron features associated with the cytolytic granular membrane.
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UR - http://www.scopus.com/inward/citedby.url?scp=0033738085&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0033738085
VL - 104
SP - 5217
EP - 5220
JO - Journal of Physical Chemistry B Materials
JF - Journal of Physical Chemistry B Materials
SN - 1520-6106
IS - 22
ER -