Near-field second harmonic imaging of granular membrane structures in natural killer cells

Richard D. Schaller, Claude Roth, David H. Raulet, Richard J. Saykally

Research output: Contribution to journalArticle

22 Citations (Scopus)


Second Harmonic Generation (SHG) has been used in combination with a near-field scanning optical microscope (NSOM) to image biological samples for the first time. Near-field SHG images of azure B stained mouse natural killer (NK) cells were recorded at four different wavelengths using a tunable near-infrared femtosecond laser. The surface specificity of SHG allows membrane structures to be selectively imaged with subdiffraction spatial resolution under ambient conditions. Resonance enhancement of SHG is shown to provide a chemically specific contrast mechanism in NSOM imaging, revealing submicron features associated with the cytolytic granular membrane.

Original languageEnglish
Pages (from-to)5217-5220
Number of pages4
JournalJournal of Physical Chemistry B
Issue number22
Publication statusPublished - Jun 8 2000


ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

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