Near-field second harmonic imaging of granular membrane structures in natural killer cells

Richard D Schaller, Claude Roth, David H. Raulet, Richard J. Saykally

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Second Harmonic Generation (SHG) has been used in combination with a near-field scanning optical microscope (NSOM) to image biological samples for the first time. Near-field SHG images of azure B stained mouse natural killer (NK) cells were recorded at four different wavelengths using a tunable near-infrared femtosecond laser. The surface specificity of SHG allows membrane structures to be selectively imaged with subdiffraction spatial resolution under ambient conditions. Resonance enhancement of SHG is shown to provide a chemically specific contrast mechanism in NSOM imaging, revealing submicron features associated with the cytolytic granular membrane.

Original languageEnglish
Pages (from-to)5217-5220
Number of pages4
JournalJournal of Physical Chemistry B
Volume104
Issue number22
Publication statusPublished - Jun 8 2000

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membrane structures
Membrane structures
Harmonic generation
harmonic generations
near fields
harmonics
Imaging techniques
optical microscopes
cells
Microscopes
Scanning
scanning
Ultrashort pulses
infrared lasers
mice
spatial resolution
membranes
Infrared radiation
Membranes
Wavelength

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Engineering(all)

Cite this

Near-field second harmonic imaging of granular membrane structures in natural killer cells. / Schaller, Richard D; Roth, Claude; Raulet, David H.; Saykally, Richard J.

In: Journal of Physical Chemistry B, Vol. 104, No. 22, 08.06.2000, p. 5217-5220.

Research output: Contribution to journalArticle

Schaller, Richard D ; Roth, Claude ; Raulet, David H. ; Saykally, Richard J. / Near-field second harmonic imaging of granular membrane structures in natural killer cells. In: Journal of Physical Chemistry B. 2000 ; Vol. 104, No. 22. pp. 5217-5220.
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