New methods in femtosecond multiphoton microscopy

Yaron Silberberg; Dvir Yelin; Dan Oron; Nirit Dudovich; Stephan Thiberge

doi:10.1117/12.478019

New methods in femtosecond multiphoton microscopy

Yaron Silberberg, Dvir Yelin, Dan Oron, Nirit Dudovich, Stephan Thiberge

Weizmann Institute of Science, Israel

Research output: Contribution to journal › Conference article › peer-review

Abstract

The field of multiphoton microscopy has undergone significant advances since its beginning just over a decade ago. One of these was the development of coherent multiphoton techniques, which typically image intrinsic properties of the sample, without resorting to staining or labeling. Here we describe some recent technological developments in the two leading coherent multiphoton techniques: third-harmonic generation and coherent anti-Stokes Raman spectroscopy. These techniques are applied for visualization of a variety of biological and other samples.

Original language	English
Pages (from-to)	209-222
Number of pages	14
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	4963
DOIs	https://doi.org/10.1117/12.478019
Publication status	Published - Sep 29 2003
Event	Multiphoton Microscopy in the Biomedical Sciences III - San Jose,CA, United States Duration: Jan 26 2003 → Jan 28 2003

Keywords

CARS
Laser-scanning
Microscopy
Nonlinear optics
THG

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

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abstract = "The field of multiphoton microscopy has undergone significant advances since its beginning just over a decade ago. One of these was the development of coherent multiphoton techniques, which typically image intrinsic properties of the sample, without resorting to staining or labeling. Here we describe some recent technological developments in the two leading coherent multiphoton techniques: third-harmonic generation and coherent anti-Stokes Raman spectroscopy. These techniques are applied for visualization of a variety of biological and other samples.",

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AU - Yelin, Dvir

AU - Oron, Dan

AU - Dudovich, Nirit

AU - Thiberge, Stephan

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AB - The field of multiphoton microscopy has undergone significant advances since its beginning just over a decade ago. One of these was the development of coherent multiphoton techniques, which typically image intrinsic properties of the sample, without resorting to staining or labeling. Here we describe some recent technological developments in the two leading coherent multiphoton techniques: third-harmonic generation and coherent anti-Stokes Raman spectroscopy. These techniques are applied for visualization of a variety of biological and other samples.

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KW - Laser-scanning

KW - Microscopy

KW - Nonlinear optics

KW - THG

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