New tetragonal form of reaction centers from Rhodobacter sphaeroides and the involvement of a manganese ion at a crystal contact point

G. Uyeda, A. Cámara-Artigas, J. C. Williams, James Paul Allen

Research output: Contribution to journalArticle

Abstract

Crystals have been obtained of wild-type reaction centers from Rhodobacter sphaeroides using manganese chloride as a precipitating agent. The crystals belong to the tetragonal space group P4222, with unit-cell parameters a = b = 207.8, c = 107.5 Å. The crystal structure has been determined to a resolution limit of 4.6 Å using a previously determined structure of the reaction center as a molecular-replacement model. The calculated electron-density maps show the presence of a manganese ion at one of the crystal contact points bridging two symmetry-related histidine residues, suggesting that the metal plays a key role in facilitating the crystallization of the protein in this form.

Original languageEnglish
Pages (from-to)733-736
Number of pages4
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume61
Issue number8
DOIs
Publication statusPublished - Aug 2005

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Rhodobacter sphaeroides
manganese ions
Molecular Models
Point contacts
Manganese
Crystallization
Histidine
Metals
Electrons
Ions
Crystals
crystals
Proteins
histidine
Crystal symmetry
Carrier concentration
manganese
Crystal structure
chlorides
crystallization

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Structural Biology
  • Genetics
  • Condensed Matter Physics

Cite this

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AU - Allen, James Paul

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