On the role of Fe2+ in bacterial photosynthesis. The effect of biosynthetic substitution of Fe2+ by Mn2+ on the electron transfer step Q- 1Q2 → Q1Q- 2 in reaction centers

Howard K. Nam, Robert H. Austin, G Charles Dismukes

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A test of the 'iron-wire' hypothesis for the role of Fe2+ in promoting the electron transfer between the primary (Q1) and secondary (Q2) quinones in bacterial reaction centers of Rhodopseudomonas sphaeroides strain R-26.1 has been conducted. Kinetics of this step, P+Q- 1Q2 → P+Q1Q- 2, and of recombination with the oxidized donor, P+Q- 1 → PQ1 and P+Q- 2 → PQ2, were followed optically at 4°C in normal iron-containing reaction centers and in reaction centers having 58% Mn2+, replacing Fe2+. This significant replacement is accomplished biosynthetically by control of the growth conditions, and so should preserve the native interactions between the cofactors. There are no significant differences observed in the recombination kinetics of the two types of reaction centers. The electron transfer between the quinones was observed to show apparent biphasic kinetics with major components of approx. 170 μs and 1.5 ms at 4°C and pH = 7.5. There is no statistically significant difference observed between the two types of reaction centers. This major change in the electronic structure of the metal and the unaltered kinetics discount the likelihood of any direct orbital participation of the metal in the electron transfer between the quinones.

Original languageEnglish
Pages (from-to)301-308
Number of pages8
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume765
Issue number3
DOIs
Publication statusPublished - Jun 26 1984

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Photosynthesis
Quinones
Substitution reactions
Electrons
Kinetics
Genetic Recombination
Iron
Metals
Rhodobacter sphaeroides
Electronic structure
Wire
Growth

Keywords

  • (Rps. sphaeroides)
  • Bacterial photosynthesis
  • Electron transfer
  • Fe effect
  • Quinone
  • Reaction center

ASJC Scopus subject areas

  • Biophysics
  • Medicine(all)

Cite this

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title = "On the role of Fe2+ in bacterial photosynthesis. The effect of biosynthetic substitution of Fe2+ by Mn2+ on the electron transfer step Q- 1Q2 → Q1Q- 2 in reaction centers",
abstract = "A test of the 'iron-wire' hypothesis for the role of Fe2+ in promoting the electron transfer between the primary (Q1) and secondary (Q2) quinones in bacterial reaction centers of Rhodopseudomonas sphaeroides strain R-26.1 has been conducted. Kinetics of this step, P+Q- 1Q2 → P+Q1Q- 2, and of recombination with the oxidized donor, P+Q- 1 → PQ1 and P+Q- 2 → PQ2, were followed optically at 4°C in normal iron-containing reaction centers and in reaction centers having 58{\%} Mn2+, replacing Fe2+. This significant replacement is accomplished biosynthetically by control of the growth conditions, and so should preserve the native interactions between the cofactors. There are no significant differences observed in the recombination kinetics of the two types of reaction centers. The electron transfer between the quinones was observed to show apparent biphasic kinetics with major components of approx. 170 μs and 1.5 ms at 4°C and pH = 7.5. There is no statistically significant difference observed between the two types of reaction centers. This major change in the electronic structure of the metal and the unaltered kinetics discount the likelihood of any direct orbital participation of the metal in the electron transfer between the quinones.",
keywords = "(Rps. sphaeroides), Bacterial photosynthesis, Electron transfer, Fe effect, Quinone, Reaction center",
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AU - Nam, Howard K.

AU - Austin, Robert H.

AU - Dismukes, G Charles

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N2 - A test of the 'iron-wire' hypothesis for the role of Fe2+ in promoting the electron transfer between the primary (Q1) and secondary (Q2) quinones in bacterial reaction centers of Rhodopseudomonas sphaeroides strain R-26.1 has been conducted. Kinetics of this step, P+Q- 1Q2 → P+Q1Q- 2, and of recombination with the oxidized donor, P+Q- 1 → PQ1 and P+Q- 2 → PQ2, were followed optically at 4°C in normal iron-containing reaction centers and in reaction centers having 58% Mn2+, replacing Fe2+. This significant replacement is accomplished biosynthetically by control of the growth conditions, and so should preserve the native interactions between the cofactors. There are no significant differences observed in the recombination kinetics of the two types of reaction centers. The electron transfer between the quinones was observed to show apparent biphasic kinetics with major components of approx. 170 μs and 1.5 ms at 4°C and pH = 7.5. There is no statistically significant difference observed between the two types of reaction centers. This major change in the electronic structure of the metal and the unaltered kinetics discount the likelihood of any direct orbital participation of the metal in the electron transfer between the quinones.

AB - A test of the 'iron-wire' hypothesis for the role of Fe2+ in promoting the electron transfer between the primary (Q1) and secondary (Q2) quinones in bacterial reaction centers of Rhodopseudomonas sphaeroides strain R-26.1 has been conducted. Kinetics of this step, P+Q- 1Q2 → P+Q1Q- 2, and of recombination with the oxidized donor, P+Q- 1 → PQ1 and P+Q- 2 → PQ2, were followed optically at 4°C in normal iron-containing reaction centers and in reaction centers having 58% Mn2+, replacing Fe2+. This significant replacement is accomplished biosynthetically by control of the growth conditions, and so should preserve the native interactions between the cofactors. There are no significant differences observed in the recombination kinetics of the two types of reaction centers. The electron transfer between the quinones was observed to show apparent biphasic kinetics with major components of approx. 170 μs and 1.5 ms at 4°C and pH = 7.5. There is no statistically significant difference observed between the two types of reaction centers. This major change in the electronic structure of the metal and the unaltered kinetics discount the likelihood of any direct orbital participation of the metal in the electron transfer between the quinones.

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