Optimizing linear polymer affinity agent properties for surface-enhanced Raman scattering detection of aflatoxin B1

Victoria M. Szlag, Rebeca S. Rodriguez, Seyoung Jung, Marc R. Bourgeois, Samuel Bryson, Anatolii Purchel, George C. Schatz, Christy L. Haynes, Theresa M. Reineke

Research output: Contribution to journalArticle

Abstract

A series of poly(N-Acryloyl glycinamide) (pNAGA) polymers were synthesized and studied as capture agents for surface-enhanced Raman scattering (SERS) detection of aflatoxin B1 (AFB1), a highly carcinogenic food-borne toxin. Four molecular weights of pNAGA were synthesized by reversible addition-fragmentation chain-Transfer (RAFT) polymerization to study the dependence of affinity agent efficacy on chain length for this AFB1 sensing platform. Isothermal titration calorimetry (ITC) was used to verify the sign and magnitude of the enthalpic effects involved in polymer-AFB1 interactions in solution and to understand the effects of pNAGA chain length on AFB1 noncovalent binding. pNAGA-AFB1 interactions were found to be exothermic, and longer pNAGA chains generally resulted in smaller enthalpy decreases per repeat unit. With pNAGA22 being thermodynamically the strongest affinity agent, we hypothesize that AFB1 affinity is determined by a balance between the configurational restrictions in pNAGA chains and the enthalpic advantage of binding AFB1. SERS spectral changes observed following AFB1 exposure were used to evaluate the influence of polymer molecular weight (2.0-5.2 kDa), order of attachment (pre-vs. post-functionalization of the substrate) and attachment chemistry (thiol vs.Trithiocarbonate) on the sensitivity of AFB1 detection. The method by which target, polymer affinity agent, and signal transduction mechanism are combined was found to have significant impacts on the achieved sensitivity. The most effective polymer chain length (pNAGA22), anchoring chemistry (thiol), and polymer/toxin assembly scheme (in-solution) allowed detection of 10 ppb AFB1 in water (below the FDA regulatory limit of 20 ppb), a hundred-fold improvement over SERS sensing without the pNAGA affinity agent.

Original languageEnglish
Pages (from-to)1019-1031
Number of pages13
JournalMolecular Systems Design and Engineering
Volume4
Issue number5
DOIs
Publication statusPublished - Oct 2019

Fingerprint

Aflatoxins
Aflatoxin B1
Raman scattering
Polymers
Chain length
Sulfhydryl Compounds
Molecular weight
Signal transduction
Calorimetry
poly(N-acryloyl glycinamide)
Titration
Enthalpy
Polymerization

ASJC Scopus subject areas

  • Chemistry (miscellaneous)
  • Chemical Engineering (miscellaneous)
  • Biomedical Engineering
  • Energy Engineering and Power Technology
  • Process Chemistry and Technology
  • Industrial and Manufacturing Engineering
  • Materials Chemistry

Cite this

Szlag, V. M., Rodriguez, R. S., Jung, S., Bourgeois, M. R., Bryson, S., Purchel, A., ... Reineke, T. M. (2019). Optimizing linear polymer affinity agent properties for surface-enhanced Raman scattering detection of aflatoxin B1. Molecular Systems Design and Engineering, 4(5), 1019-1031. https://doi.org/10.1039/c9me00032a

Optimizing linear polymer affinity agent properties for surface-enhanced Raman scattering detection of aflatoxin B1. / Szlag, Victoria M.; Rodriguez, Rebeca S.; Jung, Seyoung; Bourgeois, Marc R.; Bryson, Samuel; Purchel, Anatolii; Schatz, George C.; Haynes, Christy L.; Reineke, Theresa M.

In: Molecular Systems Design and Engineering, Vol. 4, No. 5, 10.2019, p. 1019-1031.

Research output: Contribution to journalArticle

Szlag, VM, Rodriguez, RS, Jung, S, Bourgeois, MR, Bryson, S, Purchel, A, Schatz, GC, Haynes, CL & Reineke, TM 2019, 'Optimizing linear polymer affinity agent properties for surface-enhanced Raman scattering detection of aflatoxin B1', Molecular Systems Design and Engineering, vol. 4, no. 5, pp. 1019-1031. https://doi.org/10.1039/c9me00032a
Szlag, Victoria M. ; Rodriguez, Rebeca S. ; Jung, Seyoung ; Bourgeois, Marc R. ; Bryson, Samuel ; Purchel, Anatolii ; Schatz, George C. ; Haynes, Christy L. ; Reineke, Theresa M. / Optimizing linear polymer affinity agent properties for surface-enhanced Raman scattering detection of aflatoxin B1. In: Molecular Systems Design and Engineering. 2019 ; Vol. 4, No. 5. pp. 1019-1031.
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