Orange Carotenoid Protein as a Control Element in an Antenna System Based on a DNA Nanostructure

Alessio Andreoni, Su Lin, Haijun Liu, Robert E. Blankenship, Hao Yan, Neal W. Woodbury

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Taking inspiration from photosynthetic mechanisms in natural systems, we introduced a light-sensitive photo protective quenching element to an artificial light-harvesting antenna model to control the flow of energy as a function of light intensity excitation. The orange carotenoid protein (OCP) is a nonphotochemical quencher in cyanobacteria: under high-light conditions, the protein undergoes a spectral shift, and by binding to the phycobilisome, it absorbs excess light and dissipates it as heat. By the use of DNA as a scaffold, an antenna system made of organic dyes (Cy3 and Cy5) was constructed, and OCP was assembled on it as a modulated quenching element. By controlling the illumination intensity, it is possible to switch the direction of excitation energy transfer from the donor Cy3 to either of two acceptors. Under low-light conditions, energy is transferred from Cy3 to Cy5, and under intense illumination, energy is partially transferred to OCP as well. These results demonstrate the feasibility of controlling the pathway of energy transfer using light intensity in an engineered light-harvesting system.

Original languageEnglish
Pages (from-to)1174-1180
Number of pages7
JournalNano Letters
Volume17
Issue number2
DOIs
Publication statusPublished - Feb 8 2017

Fingerprint

carotenoids
Carotenoids
Nanostructures
DNA
antennas
deoxyribonucleic acid
Antennas
proteins
Proteins
luminous intensity
illumination
energy transfer
quenching
Energy transfer
inspiration
Quenching
Lighting
excitation
energy
Excitation energy

Keywords

  • artificial light-harvesting
  • DNA nanotechnology
  • energy transfer
  • fluorescence spectroscopy
  • Orange carotenoid protein
  • photoprotection

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

Cite this

Orange Carotenoid Protein as a Control Element in an Antenna System Based on a DNA Nanostructure. / Andreoni, Alessio; Lin, Su; Liu, Haijun; Blankenship, Robert E.; Yan, Hao; Woodbury, Neal W.

In: Nano Letters, Vol. 17, No. 2, 08.02.2017, p. 1174-1180.

Research output: Contribution to journalArticle

Andreoni, Alessio ; Lin, Su ; Liu, Haijun ; Blankenship, Robert E. ; Yan, Hao ; Woodbury, Neal W. / Orange Carotenoid Protein as a Control Element in an Antenna System Based on a DNA Nanostructure. In: Nano Letters. 2017 ; Vol. 17, No. 2. pp. 1174-1180.
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