Organizing DNA origami tiles into larger structures using preformed scaffold frames

Zhao Zhao, Yan Liu, Hao Yan

Research output: Contribution to journalArticle

124 Citations (Scopus)

Abstract

Structural DNA nanotechnology utilizes DNA molecules as programmable information-coding polymers to create higher order structures at the nanometer scale. An important milestone in structural DNA nanotechnology was the development of scaffolded DNA origami in which a long single-stranded viral genome (scaffold strand) is folded into arbitrary shapes by hundreds of short synthetic oligonucleotides (staple strands). The achievable dimensions of the DNA origami tile units are currently limited by the length of the scaffold strand. Here we demonstrate a strategy referred to as "superorigami" or "origami of origami" to scale up DNA origami technology. First, this method uses a collection of bridge strands to prefold a single-stranded DNA scaffold into a loose framework. Subsequently, preformed individual DNA origami tiles are directed onto the loose framework so that each origami tile serves as a large staple. Using this strategy, we demonstrate the ability to organize DNA origami nanostructures into larger spatially addressable architectures.

Original languageEnglish
Pages (from-to)2997-3002
Number of pages6
JournalNano letters
Volume11
Issue number7
DOIs
Publication statusPublished - Jul 13 2011

Keywords

  • DNA origami
  • Structural DNA nanotechnology
  • scale up
  • self-assembly

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

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