Orientation and electronic structure of the primary donor radical cation P700+̇ in photosystem I: A single crystals EPR and ENDOR study

Hanno Käss, Petra Fromme, Horst T. Witt, Wolfgang Lubitz

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Abstract

The primary donor in single crystals of photosystem I (PS I) obtained from the thermophilic cyanobacterium Synechococcits elongatus is investigated by EPR and ENDOR techniques. The cation radical P700, was generated chemically or photolytically in the single crystals, and angular dependent spectra were obtained at a temperature of 100 K in the frozen state. The principal values and corresponding axes orientations were determined for three different proton hyperfine coupling (hfc) tensors. On the basis of the tensor magnitudes, their symmetry, and the relative orientations of their axes, they were assigned to the protons of three methyl groups of a single chlorophyll (Chl) a molecule. From X-ray crystallography of PS I single crystals, it is known that P700 is structurally a chlorophyll dimer (Klukas et al., J. Biol. Chein. 1999, 274, 7361). The EPR/ENDOR experiments show that the second chlorophyll half carries no or very little (≤15%) spin density. The molecular plane of the spin-carrying Chl a molecule in P700 is parallel to the crystallographic c axis. From the orientation of the hf tensors of the methyl groups with respect to this axis, the orientation of the Chl a molecule could be determined. The orientation of this Chl a is very similar to that of the two symmetrically placed bacteriochlorophyll molecules forming the primary donors P865 and P960 in the RC of the purple bacteria Rhodobacter sphaeroides and Rhodopseiidomonas viridis, respectively. These results indicate a structural similarity of the electron donors in purple bacteria and photosystem I and point to a common evolutionary origin of both types of reaction centers.

Original languageEnglish
Pages (from-to)1225-1239
Number of pages15
JournalJournal of Physical Chemistry B
Volume105
Issue number6
Publication statusPublished - Feb 15 2001

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Photosystem I Protein Complex
chlorophylls
Chlorophyll
Electronic structure
Paramagnetic resonance
Cations
Positive ions
Single crystals
electronic structure
Tensors
cations
Molecules
single crystals
Protons
tensors
Bacteriochlorophylls
bacteria
molecules
X ray crystallography
Dimers

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Engineering(all)

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Orientation and electronic structure of the primary donor radical cation P700+̇ in photosystem I : A single crystals EPR and ENDOR study. / Käss, Hanno; Fromme, Petra; Witt, Horst T.; Lubitz, Wolfgang.

In: Journal of Physical Chemistry B, Vol. 105, No. 6, 15.02.2001, p. 1225-1239.

Research output: Contribution to journalArticle

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