Patterned two-photon illumination by spatiotemporal shaping of ultrashort pulses

Eirini Papagiakoumou, Vincent De Sars, Dan Oron, Valentina Emiliani

Research output: Contribution to journalArticlepeer-review

100 Citations (Scopus)


Multiphoton excitation by temporally focused pulses can be combined with spatial Fourier-transform pulse shaping techniques to enhance spatial control of the excitation volume. Here we propose and demonstrate an optical system for the generation of such spatiotemporally engineered light pulses using a combination of spatial control by a two-dimensional reconfigurable light modulator, with a dispersive optical setup for temporal focusing. We show that although the properties of a holographic beam significantly differ from those of plane-wave illumination used in previous temporal focusing realizations, this leads only to a slightly reduced axial resolution. We show that the system can provide scanning-less, arbitrarily shaped, depth resolved excitation patterns that offer new perspectives for multiphoton photoactivation and optical lithography applications.

Original languageEnglish
Pages (from-to)22039-22047
Number of pages9
JournalOptics express
Issue number26
Publication statusPublished - Dec 22 2008

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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