Abstract
Erectile dysfunction (ED) critically impacts quality of life in prostatectomy, diabetic and aging patients. The underlying mechanism involves cavernous nerve (CN) damage, resulting in ED in 80% of prostatectomy patients. Peptide amphiphile (PA) nanofiber hydrogel delivery of sonic hedgehog (SHH) protein to the injured CN, improves erectile function by 60% at 6 weeks after injury, by an unknown mechanism. We hypothesize that SHH is a regulator of neurite formation. SHH treatment promoted extensive neurite formation in uninjured and crushed CNs, and SHH inhibition decreased neurites >80%. Most abundant neurites were observed with continuous SHH PA treatment of crushed CNs. Once induced with SHH, neurites continued to grow. SHH rescued neurite formation when not given immediately. SHH is a critical regulator of neurite formation in peripheral neurons under uninjured and regenerative conditions, and SHH PA treatment at the time of injury/prostatectomy provides an exploitable avenue for intervention to prevent ED.
| Original language | English |
|---|---|
| Pages (from-to) | 2087-2094 |
| Number of pages | 8 |
| Journal | Nanomedicine: Nanotechnology, Biology, and Medicine |
| Volume | 14 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - Oct 1 2018 |
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Keywords
- Cavernous nerve injury
- Neurite formation
- Pelvic ganglia
- Peptide amphiphile nanofiber hydrogel
- Peripheral nerve regeneration
- Schwann cells
- Sonic hedgehog
ASJC Scopus subject areas
- Bioengineering
- Medicine (miscellaneous)
- Molecular Medicine
- Biomedical Engineering
- Materials Science(all)
- Pharmaceutical Science
Cite this
Peptide amphiphile delivery of sonic hedgehog protein promotes neurite formation in penile projecting neurons. / Dobbs, Ryan; Choe, Shawn; Kalmanek, Elizabeth; Harrington, Daniel A.; Stupp, Samuel I; McVary, Kevin T.; Podlasek, Carol A.
In: Nanomedicine: Nanotechnology, Biology, and Medicine, Vol. 14, No. 7, 01.10.2018, p. 2087-2094.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Peptide amphiphile delivery of sonic hedgehog protein promotes neurite formation in penile projecting neurons
AU - Dobbs, Ryan
AU - Choe, Shawn
AU - Kalmanek, Elizabeth
AU - Harrington, Daniel A.
AU - Stupp, Samuel I
AU - McVary, Kevin T.
AU - Podlasek, Carol A.
PY - 2018/10/1
Y1 - 2018/10/1
N2 - Erectile dysfunction (ED) critically impacts quality of life in prostatectomy, diabetic and aging patients. The underlying mechanism involves cavernous nerve (CN) damage, resulting in ED in 80% of prostatectomy patients. Peptide amphiphile (PA) nanofiber hydrogel delivery of sonic hedgehog (SHH) protein to the injured CN, improves erectile function by 60% at 6 weeks after injury, by an unknown mechanism. We hypothesize that SHH is a regulator of neurite formation. SHH treatment promoted extensive neurite formation in uninjured and crushed CNs, and SHH inhibition decreased neurites >80%. Most abundant neurites were observed with continuous SHH PA treatment of crushed CNs. Once induced with SHH, neurites continued to grow. SHH rescued neurite formation when not given immediately. SHH is a critical regulator of neurite formation in peripheral neurons under uninjured and regenerative conditions, and SHH PA treatment at the time of injury/prostatectomy provides an exploitable avenue for intervention to prevent ED.
AB - Erectile dysfunction (ED) critically impacts quality of life in prostatectomy, diabetic and aging patients. The underlying mechanism involves cavernous nerve (CN) damage, resulting in ED in 80% of prostatectomy patients. Peptide amphiphile (PA) nanofiber hydrogel delivery of sonic hedgehog (SHH) protein to the injured CN, improves erectile function by 60% at 6 weeks after injury, by an unknown mechanism. We hypothesize that SHH is a regulator of neurite formation. SHH treatment promoted extensive neurite formation in uninjured and crushed CNs, and SHH inhibition decreased neurites >80%. Most abundant neurites were observed with continuous SHH PA treatment of crushed CNs. Once induced with SHH, neurites continued to grow. SHH rescued neurite formation when not given immediately. SHH is a critical regulator of neurite formation in peripheral neurons under uninjured and regenerative conditions, and SHH PA treatment at the time of injury/prostatectomy provides an exploitable avenue for intervention to prevent ED.
KW - Cavernous nerve injury
KW - Neurite formation
KW - Pelvic ganglia
KW - Peptide amphiphile nanofiber hydrogel
KW - Peripheral nerve regeneration
KW - Schwann cells
KW - Sonic hedgehog
UR - http://www.scopus.com/inward/record.url?scp=85050132210&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85050132210&partnerID=8YFLogxK
U2 - 10.1016/j.nano.2018.06.006
DO - 10.1016/j.nano.2018.06.006
M3 - Article
C2 - 30037776
AN - SCOPUS:85050132210
VL - 14
SP - 2087
EP - 2094
JO - Nanomedicine: Nanotechnology, Biology, and Medicine
JF - Nanomedicine: Nanotechnology, Biology, and Medicine
SN - 1549-9634
IS - 7
ER -