pH dependence of the multiline, manganese EPR signal for the 'S2' state in PS II particles. Absence of proton release during the S1 → S2 electron transfer step of the oxygen evolving system

Reddy Damoder, G Charles Dismukes

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The pH dependence of oxygen evolution rates, 2,6-dichlorophenolindophenol (DCIP) reduction rates and the intensity of the multiline manganese EPR signal associated with the S2 Kok state has been studied using oxygen-evolving spinach (PS) II particles. The oxygen evolution and DCIP reduction rates are found to be very sensitive to pH, with the maximal rates occuring at pH 6.5-7.0. Both the rate and yield of the S1 multiline manganese EPR signal intensity, produced by single flash excitation at room temperature or by continuous illumination at 200 K, are found to be independent of pH, indicating that no proton is released from this manganese site during the S1 → S1 electron transfer. These results agree with those from other laboratories showing no proton release on this transition, but using techniques monitoring other species.

Original languageEnglish
Pages (from-to)157-161
Number of pages5
JournalFEBS Letters
Volume174
Issue number1
DOIs
Publication statusPublished - Aug 20 1984

Fingerprint

Manganese
2,6-Dichloroindophenol
Paramagnetic resonance
Protons
Electrons
Oxygen
Spinacia oleracea
Lighting
Monitoring
Temperature

Keywords

  • Electron transfer
  • Manganese EPR
  • Photosynthetic oxygen evolution
  • Photosynthetic water oxidation
  • Photosystem II
  • S multiline EPR signal

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

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title = "pH dependence of the multiline, manganese EPR signal for the 'S2' state in PS II particles. Absence of proton release during the S1 → S2 electron transfer step of the oxygen evolving system",
abstract = "The pH dependence of oxygen evolution rates, 2,6-dichlorophenolindophenol (DCIP) reduction rates and the intensity of the multiline manganese EPR signal associated with the S2 Kok state has been studied using oxygen-evolving spinach (PS) II particles. The oxygen evolution and DCIP reduction rates are found to be very sensitive to pH, with the maximal rates occuring at pH 6.5-7.0. Both the rate and yield of the S1 multiline manganese EPR signal intensity, produced by single flash excitation at room temperature or by continuous illumination at 200 K, are found to be independent of pH, indicating that no proton is released from this manganese site during the S1 → S1 electron transfer. These results agree with those from other laboratories showing no proton release on this transition, but using techniques monitoring other species.",
keywords = "Electron transfer, Manganese EPR, Photosynthetic oxygen evolution, Photosynthetic water oxidation, Photosystem II, S multiline EPR signal",
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T1 - pH dependence of the multiline, manganese EPR signal for the 'S2' state in PS II particles. Absence of proton release during the S1 → S2 electron transfer step of the oxygen evolving system

AU - Damoder, Reddy

AU - Dismukes, G Charles

PY - 1984/8/20

Y1 - 1984/8/20

N2 - The pH dependence of oxygen evolution rates, 2,6-dichlorophenolindophenol (DCIP) reduction rates and the intensity of the multiline manganese EPR signal associated with the S2 Kok state has been studied using oxygen-evolving spinach (PS) II particles. The oxygen evolution and DCIP reduction rates are found to be very sensitive to pH, with the maximal rates occuring at pH 6.5-7.0. Both the rate and yield of the S1 multiline manganese EPR signal intensity, produced by single flash excitation at room temperature or by continuous illumination at 200 K, are found to be independent of pH, indicating that no proton is released from this manganese site during the S1 → S1 electron transfer. These results agree with those from other laboratories showing no proton release on this transition, but using techniques monitoring other species.

AB - The pH dependence of oxygen evolution rates, 2,6-dichlorophenolindophenol (DCIP) reduction rates and the intensity of the multiline manganese EPR signal associated with the S2 Kok state has been studied using oxygen-evolving spinach (PS) II particles. The oxygen evolution and DCIP reduction rates are found to be very sensitive to pH, with the maximal rates occuring at pH 6.5-7.0. Both the rate and yield of the S1 multiline manganese EPR signal intensity, produced by single flash excitation at room temperature or by continuous illumination at 200 K, are found to be independent of pH, indicating that no proton is released from this manganese site during the S1 → S1 electron transfer. These results agree with those from other laboratories showing no proton release on this transition, but using techniques monitoring other species.

KW - Electron transfer

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KW - Photosynthetic water oxidation

KW - Photosystem II

KW - S multiline EPR signal

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