PH-dependent structural conformations of B-phycoerythrin from Porphyridium cruentum

Ana Camara-Artigas, Julio Bacarizo, Montserrat Andujar-Sanchez, Emilia Ortiz-Salmeron, Concepcion Mesa-Valle, Celia Cuadri, Jose M. Martin-Garcia, Sergio Martinez-Rodriguez, Tania Mazzuca-Sobczuk, Maria J. Ibañez, James Paul Allen

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

B-phycoerythrin from the red alga Porphyridium cruentum was crystallized using the technique of capillary counter-diffusion. Crystals belonging to the space group R3 with almost identical unit cell constants and diffracting to 1.85 and 1.70 Å were obtained at pH values of 5 and 8, respectively. The most important difference between structures is the presence of the residue His88α in two different conformations at pH 8. This residue is placed next to the chromophore phycoerythrobilin PEB82α and the new conformation results in the relocation of the hydrogen-bond network and hydration around PEB82α, which probably contributes to the observed pH dependence of the optical spectrum associated with this chromophore. Comparison with the structures of B-phycoerythrin from other red algae shows differences in the conformation of the A-ring of the chromophore PEB139α. This conformational difference in B-phycoerythrin from P. cruentum enables the formation of several hydrogen bonds that connect PEB139α with the chromophore PEB158β at the (αβ)3 hexamer association interface. The possible influence of these structural differences on the optical spectrum and the ability of the protein to perform energy transfer are discussed, with the two pH-dependent conformations of His88α and PEB82α being proposed as representing critical structural features that are correlated with the pH dependence of the optical spectrum and transient optical states during energy transfer. Database Structural data have been deposited in the Protein Data Bank under accession numbers 3V58 and 3V57. Structured digital abstract B-phycoerythrin beta and B-phycoerythrin alpha bind by x-ray crystallography (View interaction) We have crystallized the B-phycoerythrin from the red algae Porphyridium cruentum at the pH 5 and 8. The quality of the measured diffraction data has allowed us to perform a detailed modeling of the cofactors and the solvent, enabling the detection of some changes that are proposed to be important for the function of this protein as a light-harvesting system

Original languageEnglish
Pages (from-to)3680-3691
Number of pages12
JournalFEBS Journal
Volume279
Issue number19
DOIs
Publication statusPublished - Oct 2012

Fingerprint

Porphyridium
Phycoerythrin
Conformations
Chromophores
Rhodophyta
Algae
Energy Transfer
Energy transfer
Hydrogen
Hydrogen bonds
Databases
Crystallography
Proteins
Relocation
Hydration
Diffraction
X-Rays
Association reactions
Light
X rays

Keywords

  • B-phycoerythrin
  • capillary counter-diffusion
  • crystal structure
  • photosynthesis
  • Porphyridium cruentum

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Camara-Artigas, A., Bacarizo, J., Andujar-Sanchez, M., Ortiz-Salmeron, E., Mesa-Valle, C., Cuadri, C., ... Allen, J. P. (2012). PH-dependent structural conformations of B-phycoerythrin from Porphyridium cruentum. FEBS Journal, 279(19), 3680-3691. https://doi.org/10.1111/j.1742-4658.2012.08730.x

PH-dependent structural conformations of B-phycoerythrin from Porphyridium cruentum. / Camara-Artigas, Ana; Bacarizo, Julio; Andujar-Sanchez, Montserrat; Ortiz-Salmeron, Emilia; Mesa-Valle, Concepcion; Cuadri, Celia; Martin-Garcia, Jose M.; Martinez-Rodriguez, Sergio; Mazzuca-Sobczuk, Tania; Ibañez, Maria J.; Allen, James Paul.

In: FEBS Journal, Vol. 279, No. 19, 10.2012, p. 3680-3691.

Research output: Contribution to journalArticle

Camara-Artigas, A, Bacarizo, J, Andujar-Sanchez, M, Ortiz-Salmeron, E, Mesa-Valle, C, Cuadri, C, Martin-Garcia, JM, Martinez-Rodriguez, S, Mazzuca-Sobczuk, T, Ibañez, MJ & Allen, JP 2012, 'PH-dependent structural conformations of B-phycoerythrin from Porphyridium cruentum', FEBS Journal, vol. 279, no. 19, pp. 3680-3691. https://doi.org/10.1111/j.1742-4658.2012.08730.x
Camara-Artigas A, Bacarizo J, Andujar-Sanchez M, Ortiz-Salmeron E, Mesa-Valle C, Cuadri C et al. PH-dependent structural conformations of B-phycoerythrin from Porphyridium cruentum. FEBS Journal. 2012 Oct;279(19):3680-3691. https://doi.org/10.1111/j.1742-4658.2012.08730.x
Camara-Artigas, Ana ; Bacarizo, Julio ; Andujar-Sanchez, Montserrat ; Ortiz-Salmeron, Emilia ; Mesa-Valle, Concepcion ; Cuadri, Celia ; Martin-Garcia, Jose M. ; Martinez-Rodriguez, Sergio ; Mazzuca-Sobczuk, Tania ; Ibañez, Maria J. ; Allen, James Paul. / PH-dependent structural conformations of B-phycoerythrin from Porphyridium cruentum. In: FEBS Journal. 2012 ; Vol. 279, No. 19. pp. 3680-3691.
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