Pharmacokinetics of ICG and HPPH-car for the detection of normal and tumor tissue using fluorescence, near-infrared reflectance imaging

A case study

Michael Gurfinkel, Alan B. Thompson, William Ralston, Tamara L. Troy, Ana L Moore, Thomas A Moore, John Devens Gust, Derreck Tatman, Jeffery S. Reynolds, Bruce Muggenburg, Kristin Nikula, Ravindra Pandey, Ralf H. Mayer, Daniel J. Hawrysz, Eva M. Sevick-Muraca

Research output: Contribution to journalArticle

145 Citations (Scopus)

Abstract

We present in vivo fluorescent, near-infrared (NIR), reflectance images of indocyanine green (ICG) and carotene-conjugated 2-devinyl-2-(1-hexyloxyethyl) pyropheophorbide (HPPH-car) to discriminate spontaneous canine adenocarcinoma from normal mammary tissue. Following intravenous administration of 1.0 mg kg-1 ICG or 0.3 mg kg-1 HPPH-car into the canine, a 25 mW, 778 nm or 70 mW, 660 nm laser diode beam, expanded by a diverging lens to approximately 4 cm in diameter, illuminated the surface of the mammary tissue. Successfully propagating to the tissue surface, ICG or HPPH-car fluorescence generated from within the tissue was collected by an image-intensified, charge-coupled device camera fitted with an 830 or 710 nm bandpass interference filter. Upon collecting time-dependent fluorescence images at the tissue surface overlying both normal and diseased tissue volumes, and fitting these images to a pharmacokinetic model describing the uptake (wash-in) and release (wash-out) of fluorescent dye, the pharmacokinetics of fluorescent dye was spatially determined. Mapping the fluorescence intensity owing to ICG indicates that the dye acts as a blood pool or blood persistent agent, for the model parameters show no difference in the ICG uptake rates between normal and diseased tissue regions. The wash-out of ICG was delayed for up to 72 h after intravenous injection in tissue volumes associated with disease, because ICG fluorescence was still detected in the diseased tissue 72 h after injection. In contrast, HPPH-car pharmacokinetics illustrated active uptake into diseased tissues, perhaps owing to the overexpression of LDL receptors associated with the malignant cells. HPPH-car fluorescence was not discernable after 24 h. This work illustrates the ability to monitor the pharmacokinetic delivery of NIR fluorescent dyes within tissue volumes as great as 0.5-1 cm from the tissue surface in order to differentiate normal from diseased tissue volumes on the basis of parameters obtained from the pharmacokinetic models.

Original languageEnglish
Pages (from-to)94-102
Number of pages9
JournalPhotochemistry and Photobiology
Volume72
Issue number1
DOIs
Publication statusPublished - Jul 1 2000

Fingerprint

Indocyanine Green
Pharmacokinetics
Tumors
Railroad cars
tumors
Fluorescence
Tissue
Infrared radiation
reflectance
Imaging techniques
fluorescence
Neoplasms
dyes
Fluorescent Dyes
2-(1-hexyloxyethyl)-2-devinyl pyropheophorbide-a
blood
Canidae
Breast
Blood
injection

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics

Cite this

Pharmacokinetics of ICG and HPPH-car for the detection of normal and tumor tissue using fluorescence, near-infrared reflectance imaging : A case study. / Gurfinkel, Michael; Thompson, Alan B.; Ralston, William; Troy, Tamara L.; Moore, Ana L; Moore, Thomas A; Gust, John Devens; Tatman, Derreck; Reynolds, Jeffery S.; Muggenburg, Bruce; Nikula, Kristin; Pandey, Ravindra; Mayer, Ralf H.; Hawrysz, Daniel J.; Sevick-Muraca, Eva M.

In: Photochemistry and Photobiology, Vol. 72, No. 1, 01.07.2000, p. 94-102.

Research output: Contribution to journalArticle

Gurfinkel, M, Thompson, AB, Ralston, W, Troy, TL, Moore, AL, Moore, TA, Gust, JD, Tatman, D, Reynolds, JS, Muggenburg, B, Nikula, K, Pandey, R, Mayer, RH, Hawrysz, DJ & Sevick-Muraca, EM 2000, 'Pharmacokinetics of ICG and HPPH-car for the detection of normal and tumor tissue using fluorescence, near-infrared reflectance imaging: A case study', Photochemistry and Photobiology, vol. 72, no. 1, pp. 94-102. https://doi.org/10.1562/0031-8655(2000)072<0094:POIAHC>2.0.CO;2
Gurfinkel, Michael ; Thompson, Alan B. ; Ralston, William ; Troy, Tamara L. ; Moore, Ana L ; Moore, Thomas A ; Gust, John Devens ; Tatman, Derreck ; Reynolds, Jeffery S. ; Muggenburg, Bruce ; Nikula, Kristin ; Pandey, Ravindra ; Mayer, Ralf H. ; Hawrysz, Daniel J. ; Sevick-Muraca, Eva M. / Pharmacokinetics of ICG and HPPH-car for the detection of normal and tumor tissue using fluorescence, near-infrared reflectance imaging : A case study. In: Photochemistry and Photobiology. 2000 ; Vol. 72, No. 1. pp. 94-102.
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AU - Troy, Tamara L.

AU - Moore, Ana L

AU - Moore, Thomas A

AU - Gust, John Devens

AU - Tatman, Derreck

AU - Reynolds, Jeffery S.

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AU - Nikula, Kristin

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AU - Mayer, Ralf H.

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